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Journal of Gastroenterology

, Volume 48, Issue 2, pp 193–202 | Cite as

Hepatocyte growth factor stimulates the migration of gastric epithelial cells by altering the subcellular localization of the tight junction protein ZO-1

  • Yuichiro Nasu
  • Akio IdoEmail author
  • Shirou Tanoue
  • Shinichi Hashimoto
  • Fumisato Sasaki
  • Shuji Kanmura
  • Hitoshi Setoyama
  • Masatsugu Numata
  • Keita Funakawa
  • Akihiro Moriuchi
  • Hiroshi Fujita
  • Toshio Sakiyama
  • Hirofumi Uto
  • Makoto Oketani
  • Hirohito Tsubouchi
Original Article—Alimentary Tract

Abstract

Background

Hepatocyte growth factor (HGF) is essential for epithelial restitution, a process in which epithelial cells rapidly migrate to cover desquamated epithelium after mucosal injury in the gastrointestinal tract. In this study, we aimed to elucidate the molecular mechanisms of the HGF-mediated reconstitution of gastric epithelial structures by analyzing the expression and subcellular dynamics of tight junction proteins.

Methods

We treated human gastric epithelial MKN74 cells with HGF, and examined the effects of HGF on cell migration and proliferation, and the expression and subcellular dynamics of tight junction proteins; as well, we investigated the effect of HGF on paracellular permeability to macromolecules (using fluorescein isothiocyanate [FITC]-dextran).

Results

HGF significantly stimulated the migration of MKN74 cells, but not their proliferation, in a dose-dependent manner. HGF did not affect the expression of tight junction proteins, including claudin-1, -3, -4 and -7; occludin; and zonula occludens (ZO)-1. However, fluorescence immunostaining revealed that, in the cell membrane, the levels of ZO-1, but not those of occludin or claudin-4, were transiently decreased 1 h after HGF treatment. The results were further confirmed by western blotting: HGF reduced the amount of ZO-1 protein in the cell membrane fraction concomitantly with an increase in cytoplasmic ZO-1. Furthermore, HGF reduced the interaction between ZO-1 and occludin, and induced the tyrosine phosphorylation of occludin, whereas the phosphorylation status of ZO-1 was not affected by exposure to HGF. Despite a decrease in the ZO-1/occludin interaction, HGF did not affect paracellular permeability to macromolecules.

Conclusions

HGF alters the subcellular localization of ZO-1, probably through the tyrosine phosphorylation of occludin, which may induce cell dispersion during epithelial restitution.

Keywords

HGF Migration Epithelial restitution Mucosal injury Tight junction 

Notes

Acknowledgments

The authors thank Ms. Yuko Morinaga-Nakamura for technical assistance. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan; and by Grants-in-Aid from the Ministry of Health, Labour and Welfare of Japan.

Conflicts of interest

The authors declare no conflicts of interest.

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Copyright information

© Springer 2012

Authors and Affiliations

  • Yuichiro Nasu
    • 1
  • Akio Ido
    • 1
    Email author
  • Shirou Tanoue
    • 1
  • Shinichi Hashimoto
    • 1
  • Fumisato Sasaki
    • 1
  • Shuji Kanmura
    • 1
  • Hitoshi Setoyama
    • 1
  • Masatsugu Numata
    • 1
  • Keita Funakawa
    • 1
  • Akihiro Moriuchi
    • 1
  • Hiroshi Fujita
    • 1
  • Toshio Sakiyama
    • 1
  • Hirofumi Uto
    • 1
  • Makoto Oketani
    • 1
  • Hirohito Tsubouchi
    • 1
  1. 1.Digestive Disease and Life-style Related DiseaseKagoshima University Graduate School of Medical and Dental SciencesKagoshimaJapan

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