Journal of Gastroenterology

, Volume 47, Issue 12, pp 1298–1307 | Cite as

Multicenter analysis of fecal microbiota profiles in Japanese patients with Crohn’s disease

  • Akira AndohEmail author
  • Hiroyuki Kuzuoka
  • Tomoyuki Tsujikawa
  • Shiro Nakamura
  • Fumihito Hirai
  • Yasuo Suzuki
  • Toshiyuki Matsui
  • Yoshihide Fujiyama
  • Takayuki Matsumoto
Original Article—Alimentary Tract



We analyzed the fecal microbiota profiles of patients with Crohn’s disease (CD) at 4 inflammatory bowel disease (IBD) centers located in different districts in Japan.


Terminal restriction fragment length polymorphism (T-RFLP) analysis was performed in 161 fecal samples from CD patients and 121 samples from healthy individuals. The bacterial diversity was evaluated by the Shannon diversity index (SDI).


There were no regional differences in the fecal microbiota profiles of the healthy individuals in Japan. A setting of similarity generated three major clusters of T-RFs: one included almost all the healthy individuals (118/121), and the other two clusters were mainly formed by CD patients at different stages of disease activity. The changes in simulated bacterial composition indicated that the class Clostridia, including the genus Faecalibacterium, was significantly decreased in CD patients with active disease and those in remission as compared with findings in the healthy individuals. In contrast, the genus Bacteroides was significantly increased in CD patients during the active phase as compared with findings in the healthy individuals. The genus Bifidobacterium was significantly decreased during the active phase of CD and increased to healthy levels during the remission phase. The bacterial diversity measured by the SDI was significantly reduced in CD patients during the active and remission phases as compared with findings in the healthy individuals. From the clinical data and T-RFLP analysis, we developed a logistic model to predict disease activity based on the fecal microbiota composition.


Dysbiosis in CD patients was shown by a multi-IBD center study. The feasibility of using the fecal microbiota profile as a predictive marker for disease activity is proposed.


Inflammatory bowel disease Faecalibacterium Firmicutes Shannon diversity index T-RFLP 



The authors appreciate the technical support of TechnoSuruga Laboratory Co., Ltd. (Shizuoka, Japan) and would like to express their thanks.

Conflict of interest

The authors have no conflict of interests to declare in this study.

Supplementary material

535_2012_605_MOESM1_ESM.pptx (238 kb)
Supplementary material 1 (PPTX 237 kb)


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Copyright information

© Springer 2012

Authors and Affiliations

  • Akira Andoh
    • 1
    Email author
  • Hiroyuki Kuzuoka
    • 2
    • 3
  • Tomoyuki Tsujikawa
    • 6
  • Shiro Nakamura
    • 2
  • Fumihito Hirai
    • 4
  • Yasuo Suzuki
    • 5
  • Toshiyuki Matsui
    • 4
  • Yoshihide Fujiyama
    • 6
  • Takayuki Matsumoto
    • 2
  1. 1.Division of Mucosal ImmunologyGraduate School, Shiga University of Medical ScienceOtsuJapan
  2. 2.Department of Lower GastroenterologyHyogo College of MedicineNishinomiyaJapan
  3. 3.Research and Development LaboratoriesEN Otsuka Pharmaceutical Co., Ltd.HanamakiJapan
  4. 4.Department of GastroenterologyFukuoka University Chikushi HospitalChikushinoJapan
  5. 5.Department of Internal MedicineToho University Sakura Medical CenterSakuraJapan
  6. 6.Department of MedicineShiga University of Medical ScienceOtsuJapan

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