Journal of Gastroenterology

, Volume 45, Issue 11, pp 1103–1110

Induction of intestinal ischemia reperfusion injury by portal vein outflow occlusion in rats

  • Marco Vincenti
  • Matthias Behrends
  • Kim Dang
  • Yeon H. Park
  • Ryutaro Hirose
  • Annabel Blasi-Ibanez
  • Tao Liu
  • Natalie J. Serkova
  • Claus U. Niemann
Original Article—Alimentary Tract

DOI: 10.1007/s00535-010-0262-0

Cite this article as:
Vincenti, M., Behrends, M., Dang, K. et al. J Gastroenterol (2010) 45: 1103. doi:10.1007/s00535-010-0262-0

Abstract

Background

Intestinal ischemia can occur from mesenteric artery (MA) occlusion and portal vein (PV) occlusion. The degree and mechanisms of ischemia/reperfusion (I/R) injury in these conditions may differ. Metabolic changes are seen early in I/R. This study compares tissue histology, inflammation, and metabolic response during small bowel I/R due to superior MA or PV occlusion.

Methods

Anesthetized male Wistar rats (250–300 g) underwent laparotomy followed by MA or PV occlusion for 40 min. After 120 min of reperfusion, small bowel tissue was collected. The expression of heat shock protein (HSP)-32 and HSP70 was evaluated to compare physiological stress responses between groups. Metabolic profiles were obtained using 1H-nuclear magnetic resonance spectroscopy (NMR)-based quantitative metabolomics. Histological injury of small bowel was graded from 0 (normal) to 4 (extensive ischemic damage).

Results

Protein expression of HSP32 and HSP70 increased when compared to sham but was not different in the MA I/R and PV I/R groups. Metabolic profiles demonstrated decreased glucose levels and highly elevated tissue lactate and amino acids and fatty acids following I/R, with more pronounced changes with PV occlusion. Lipid peroxidation was equally increased in both groups, while depletion of reduced glutathione (GSH) was more severe with MA occlusion. The epithelial necrosis score was higher with MA (3.5 ± 0.6) than with PV occlusion (2.3 ± 0.8).

Conclusions

Histological injury of the intestine is less pronounced following PV occlusion, most likely due to higher oxygen and substrate availability during I/R by PV occlusion. This conclusion is supported by a more pronounced metabolic synthetic response (increased glycolysis and fatty acid and amino acid accumulation) with PV occlusion, while oxidative stress was higher with MA occlusion. The inflammatory response showed little difference between the groups.

Keywords

Intestinal ischemia/reperfusion injury Mesenteric artery occlusion Portal vein occlusion Metabolomics Heat shock proteins 

Abbreviations

I/R

Ischemia–reperfusion

HSP32

Heat shock protein-32

HSP70

Heat shock protein-70

NMR

Nuclear magnetic resonance spectroscopy

Copyright information

© Springer 2010

Authors and Affiliations

  • Marco Vincenti
    • 1
  • Matthias Behrends
    • 2
  • Kim Dang
    • 3
  • Yeon H. Park
    • 4
  • Ryutaro Hirose
    • 1
  • Annabel Blasi-Ibanez
    • 5
  • Tao Liu
    • 1
  • Natalie J. Serkova
    • 6
  • Claus U. Niemann
    • 1
    • 2
  1. 1.Department of Surgery, Division of TransplantationUniversity of CaliforniaSan FranciscoUSA
  2. 2.Department of Anesthesia and Perioperative CareUniversity of CaliforniaSan FranciscoUSA
  3. 3.Stanford University School of MedicineStanfordUSA
  4. 4.Department of SurgeryGachon Medical School, Gil Medical CenterIncheonKorea
  5. 5.Servei d’ Anestesiologia i Reanimacio, Hospital ClinicBarcelonaSpain
  6. 6.Department of Anesthesiology, Biomedical MRI/PET/CT Cancer Center CoreUniversity of Colorado Health Sciences CenterAuroraUSA

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