A phylogenetic study of hepatitis B virus in chronically infected Brazilian patients of Western and Asian descent
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Hepatitis B virus (HBV) causes one of the most important chronic viral infections worldwide. HBV is classified into eight genotypes whose epidemiology varies geographically. In Brazil, genotypes A, D, and F are more frequent, while in East Asia, genotypes B and C predominate. Several studies showed that immigrants retain the HBV infection pattern of their ancestral country.
To identify HBV genotypes infecting chronic carriers in Brazilian families of Western and Asian descent by Hepatitis B surface antigen gene sequencing and analyze the route of viral transmission by phylogenetic analysis of viral sequences.
Eighty-seven people chronically infected with HBV were separated into two groups: Western descent (27) and Asian descent (60). Surface and pre-core/core genes were amplified from serum HBV-DNA and sequences were subjected to phylogenetic analysis.
HBV genotype A was found in 74% of Western subjects, while genotype C was found in 94% of Asian patients. Thirty-eight percent of Western families were infected with HBV with similar pre-core/core sequences, while only 25% of Asian families showed similarity in these sequences.
Phylogenetical analysis of pre-core/core HBV gene suggested intra-familial transmission of HBV in 38% of Western families and 25% of Asian families. Analysis of HBsAg gene sequences helped to define the HBV genotype but did not allow inferring route of transmission as its sequences showed a smaller phylogenetic signal than pre-core/core sequences. Chronic HBV carriers of Asian descent born in or living in Brazil were infected with the same HBV genotype predominant in their ancestral country.
KeywordsHepatitis B Disease transmission Genotype Phylogeny Family
FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo (Grants 00/00998/1 and 0011457/1 Viral Genetic Diversity Network) and Alves de Queiroz Family Fund for Research.
- 1.Lavanchy D. Epidemiology. In: Thomas HC, Lemon S, Zuckerman AJ, editors. Viral Hepatitis. 3rd ed. Malden: Blackwell; 2005. p. 181–92.Google Scholar
- 2.Kann M, Gerlich WH. Structure and molecular virology. In: Thomas HC, Lemon S, Zuckerman AJ, editors. Viral Hepatitis. 3rd ed. Malden: Blackwell; 2005. p. 149–80.Google Scholar
- 5.Carrilho FJ, Mendes-Corrêa MCJ. Magnitude of hepatitis B and C in Latin America. In: Schinazi RF, Sommadossi J-P, Thomas HC, editors. Therapies for Viral Hepatitis. London: International Medical Press; 1998. p. 25–34.Google Scholar
- 13.Hall T. BioEdit, version 6.0.7. Ibis therapeutics. Carlsbad, 2004.Google Scholar
- 14.Swofford DL. PAUP* Phylogenetic analysis using Parsimony (*and Other Methods), Version 4b10. Sinauer. Sunderland, 2002.Google Scholar