Journal of Gastroenterology

, Volume 44, Issue 6, pp 556–561

Down-regulation of miR-141 in gastric cancer and its involvement in cell growth

Original Article—Alimentary Tract

Abstract

Purpose

Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation.

Methods

The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay.

Results

MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells.

Conclusions

These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.

Keywords

MiR-141 Gastric cancer MGC-803 cell Cell proliferation 

Supplementary material

535_2009_37_MOESM1_ESM.doc (28 kb)
Supplementary Table S1 (DOC 28 kb)
535_2009_37_MOESM2_ESM.eps (8.3 mb)
Supplementary Fig. S1 (EPS 8496 kb)

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Copyright information

© Springer 2009

Authors and Affiliations

  1. 1.Gastroenterology Laboratory, Clinical Research Institute, Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhouPeople’s Republic of China
  2. 2.The Center for Diseases ModelingZhejiang University School of MedicineHangzhouPeople’s Republic of China

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