Down-regulation of miR-141 in gastric cancer and its involvement in cell growth
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Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation.
The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay.
MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells.
These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.
KeywordsMiR-141 Gastric cancer MGC-803 cell Cell proliferation
We thank Xunyan Liu for her technical help. The study was supported by a grant from the National High Technology Research and Development Program of China (No. 2006AA02Z470), a joint grant from National Ministry of Public Health and Zhejiang Province (WKJ2006-2-012 and -014), a grant from the Medicine Science Foundation of Zhejiang Province (No. 2008A041), a grant from National Ministry of Education (NCET-06-0530) and two grants from Natural Scientific Foundation of Zhejiang Province (R205291 and 2007R10G2010103).