In vivo recording of colonic motility in conscious rats with deficiency of interstitial cells of Cajal, with special reference to the effects of nitric oxide on colonic motility
- 49 Downloads
We recorded in vivo colonic motility in rats with a deficiency of interstitial cells of Cajal (ICC) (Ws/Ws rats) and in wild-type rats (+/+ rats), with special reference to the effects of nitric oxide (NO) on colonic motility in both types of rats, in order to ascertain the role of ICC in colonic motility, and the relationship between NO and ICC in regard to colonic motility.
Miniature strain-gauge force transducers were sutured on the surface of the ascending and sigmoid colon of Ws/Ws rats and +/+ rats as controls. After 1 week and a fasting period of 24 h, colonic motility in +/+ and Ws/Ws rats was recorded. We also studied the effect of NO on colonic motility in both types of rats, by means of the administration of N-nitro-l-arginine methyl ester (l-NAME) or l-arginine.
In +/+ rats, there were contractions with high amplitude and long duration in both the ascending and sigmoid colon. The number, amplitude, and duration of contractions in the ascending colon were 9.9/20 min, 6.1 g, and 22.7 s, respectively. These findings in the sigmoid colon were 5.2/20 min, 5.2 g, and 23.0 s, respectively. The number of contractions in the ascending and sigmoid colon in Ws/Ws rats (2.3 and 1.0/20 min) was significantly lower than that in +/+ rats (P < 0.05). The number of contractions in the ascending and sigmoid colon in +/+ rats (9.7 and 5.1/20 min before treatment) was significantly increased by l-NAME administration (28.7 and 13.9/40–60 min after treatment; P < 0.05), but that in Ws/Ws rats was not influenced. The number of contractions in the ascending and sigmoid colon in +/+ rats (10.2 and 5.2/20 min before treatment) was significantly decreased by l-arginine administration (3.6 and 2.1/40–60 min after treatment; P < 0.05), but that in Ws/Ws rats was not influenced.
ICC must be related to the occurrence of a normal number of colonic contractions. NO may be involved in the inhibitory regulation of colonic motility, and the effect of NO on the occurrence of contractions appears to be mediated by ICC.
Key wordscolonic motility colonic contraction interstitial cells of Cajal nitric oxide
Unable to display preview. Download preview PDF.
- 4.Ward, SM, Burns, AJ, Torihashi, S, Sanders, KM 1994Mutation of the protooncogene c-kit blocks development of intestinal cells and electrical rhythmicity in murine intestineJ Physiol (Lond)480917Google Scholar
- 6.Ward, SM, Burns, AJ, Torihashi, S, Harney, SC, Sandes, K 1995Impaired development of interstitial cells and intestinal electrical rhythmicity in steel mutantsAm J Physiol Cell Physiol269C157785Google Scholar
- 8.Koh, SD, Kim, TW, Jun, JY, Glasgow, NJ, Ward, SM, Sanders, KM 2000Regulation of pacemaker currents in interstitial cells of Cajal from murine small intestine by cyclic nucleotidesJ Physiol527.114962Google Scholar
- 16.Itoh, Z, Sekiguchi, T 1983Interdigestive motor activity in health and diseaseScand J Gastroenterol1812134Google Scholar
- 17.Itoh, Z, Nakaya, M, Suzuki, T, Arai, H, Wakabayashi, K 1984Erythromycin mimics exogenous motilin in gastrointestinal contractile activity in the dogAm J Physiol Gastrointest Liver Physiol247G68894Google Scholar
- 20.Ward, SM, Dalziel, HH, Thornbury, KD, Westfall, DP, Sanders, KM 1992Nonadrenergic, noncholinergic inhibition and rebound excitation in canine colon depend on nitric oxideAm J Physiol Gastrointest Liver Physiol262G23743Google Scholar
- 21.Venkova, K, Krier, J 1994A nitric oxide and prostaglandin-dependent component of NANC off-contractions in cat colonAm J Physiol Gastrointest Liver Physiol266G407Google Scholar