Journal of Gastroenterology

, Volume 40, Issue 8, pp 802–810

Interleukin-17 augments tumor necrosis factor-α-induced granulocyte and granulocyte/macrophage colony-stimulating factor release from human colonic myofibroblasts

  • Akira Andoh
  • Hirofumi Yasui
  • Osamu Inatomi
  • Zhuobin Zhang
  • Yasuyuki Deguchi
  • Kazunori Hata
  • Yoshio Araki
  • Tomoyuki Tsujikawa
  • Katsuyuki Kitoh
  • Shokei Kim-Mitsuyama
  • Atsushi Takayanagi
  • Nobuyoshi Shimizu
  • Yoshihide Fujiyama
Article

DOI: 10.1007/s00535-005-1632-x

Cite this article as:
Andoh, A., Yasui, H., Inatomi, O. et al. J Gastroenterol (2005) 40: 802. doi:10.1007/s00535-005-1632-x

Abstract

Background

Interleukin (IL)-17 is a newly identified T-cell-specific cytokine. In this study, we investigated the effects of IL-17 on colony-stimulating factor (CSF) release in human colonic subepithelial myofibroblasts (SEMFs).

Methods

CSF release and mRNA expression were determined by enzyme-linked immunosorbent assay (ELISA) and Northern blotting, respectively. Nuclear factor (NF)-κB- and activating protein (AP-1)-DNA binding activities were evaluated by electrophoretic gel mobility shift assays (EMSAs).

Results

Unstimulated cells secreted a small amount of granulocyte G- and granulocyte/macrophage (GM)-CSF, and a considerable amount of M-CSF. IL-17 weakly enhanced G-CSF release, but did not affect GM- and M-CSF release. IL-17 selectively enhanced tumor necrosis factor (TNF)-α-induced G- and GM-CSF release. The combination of IL-17 plus TNF-α induced a marked increase in NF-κB- and AP-1-DNA binding activities. The adenovirus-mediated transfer of a stable form of IκBα and/or a dominant negative mutant of c-Jun markedly inhibited the IL-17 plus TNF-α-induced G- and GM-CSF mRNA expression. Furthermore, a stability study showed that IL-17 plus TNF-α markedly enhanced the stability of G- and GM-CSF mRNA.

Conclusions

IL-17 augments TNF-α-induced G- and GM-CSF release via transcriptional and posttranscriptional mechanisms.

Key words

M-CSF T cells NF-κB AP-1 

Copyright information

© Springer-Verlag Tokyo 2005

Authors and Affiliations

  • Akira Andoh
    • 1
  • Hirofumi Yasui
    • 1
  • Osamu Inatomi
    • 1
  • Zhuobin Zhang
    • 1
  • Yasuyuki Deguchi
    • 1
  • Kazunori Hata
    • 1
  • Yoshio Araki
    • 1
  • Tomoyuki Tsujikawa
    • 1
  • Katsuyuki Kitoh
    • 1
  • Shokei Kim-Mitsuyama
    • 2
  • Atsushi Takayanagi
    • 3
  • Nobuyoshi Shimizu
    • 3
  • Yoshihide Fujiyama
    • 1
  1. 1.Department of Internal MedicineShiga University of Medical ScienceOtsuJapan
  2. 2.Department of Pharmacology and Molecular TherapeuticsKumamoto University Graduate School of Medical SciencesKumamotoJapan
  3. 3.Department of Molecular BiologyKeio University School of MedicineTokyoJapan

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