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Journal of Hepato-Biliary-Pancreatic Surgery

, Volume 14, Issue 5, pp 493–497 | Cite as

Green-tea polyphenol (-)-epigallocatechin-3-gallate provides resistance to apoptosis in isolated islets

  • Yuko Hara
  • Masayuki Fujino
  • Masao Takeuchi
  • Xiao-Kang Li
Original articles

Abstract

Background/Purpose

Apoptosis resulting from disruption of the normal cell-matrix relationship (anoikis) during islet isolation, and the reactive oxygen and nitrogen species generated following hypoxia/reoxygenation (H/R) can lead to a loss of islet tissue in culture and the reduced survival of transplanted pancreatic islets. The aim of this study was to investigate the effect of (-)-epigallocatechin-3-gallate (EGCG), a well-known antiapoptotic agent, on inhibiting anoikis and H/R injury in an in vitro islet culture system.

Methods

Islets were isolated from F344 rats and cultured under normal or H/R condition with/without EGCG.

Results

EGCG inhibited apoptosis and lactate-dehydrogenase leakage from anoikis and H/R in a dose-dependent manner. Further, EGCG prevent increases in 8-hydroxy-2′-deoxyguanosine content and inhibited the decline of insulin secretory function induced by H/R.

Conclusions

These results suggest that the addition of EGCG to an islet culture system may improve the survival rate of isolated islets and reduce the loss of functional islet mass that compromises the stable reversal of diabetes after islet transplantation.

Key words

(-)-Epigallocatechin-3-gallate Islet Apoptosis Anoikis Hypoxia/Reoxygenation 

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Copyright information

© Springer-Verlag Tokyo 2007

Authors and Affiliations

  • Yuko Hara
    • 1
  • Masayuki Fujino
    • 1
    • 2
  • Masao Takeuchi
    • 3
  • Xiao-Kang Li
    • 1
  1. 1.National Research Institute for Child Health and DevelopmentLaboratory of Transplantation ImmunologyTokyoJapan
  2. 2.AIDS Research CenterNational Institute of Infectious DiseasesTokyoJapan
  3. 3.Japan Institute for The Control of AgingNikken Seil CorporationShizuokaJapan

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