Hepatocyte differentiation from embryonic stem cells and umbilical cord blood cells

  • Kenichi Teramoto
  • Kinji Asahina
  • Yuji Kumashiro
  • Sei Kakinuma
  • Ryoko Chinzei
  • Keiko Shimizu-Saito
  • Yujiro Tanaka
  • Hirobumi Teraoka
  • Shigeki Arii
Article

Abstract

With the development of regeneration medicine, many researchers have attempted hepatic differentiation from nonhepatic-origin cell sources. The differentiation of embryonic stem (ES) cells into hepatocyte-like cells has been reported in several papers. Mouse ES cells have shown a potential to develop into hepatocyte-like cells in vitro on the basis of hepatic gene expression after adding several growth factors. We transplanted cultured embryoid body (EB) cells (male) into female mice. A liver specimen of the recipient was examined by immunohistochemical staining for albumin and fluorescence in situ hybridization for the Y chromosome after transplantation. Both Y chromosome- and albumin-positive cells were recognized in the recipient female liver, and were considered to be hepatocyte-like cells derived from ES cells containing the Y chromosome. Many groups, including ourselves, have studied hepatocyte-like cell differentiation from umbilical cord blood cells (UBCs). We cultured nucleated cells isolated from UBCs. Using immunostaining, ALB-positive and CK-19-positive cells were recognized in the culture. Dual staining of ALB and CK-19 demonstrated that ALB was coexpresed with CK-19, suggesting the existence of hepatic progenitors. In this review, we consider recent studies of the differentiation of hepatocytes from nonhepatic origins, especially ES cells and umbilical cord blood.

Key words

ES cell Umbilical cord blood cell Hepatocyte differentiation Liver failure Hepatocyte transplantation 

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Copyright information

© Springer-Verlag Tokyo 2005

Authors and Affiliations

  • Kenichi Teramoto
    • 1
  • Kinji Asahina
    • 2
  • Yuji Kumashiro
    • 1
  • Sei Kakinuma
    • 3
  • Ryoko Chinzei
    • 3
  • Keiko Shimizu-Saito
    • 2
  • Yujiro Tanaka
    • 3
  • Hirobumi Teraoka
    • 2
  • Shigeki Arii
    • 1
  1. 1.Department of Hepato-Biliary-Pancreatic Surgery, Graduate School of Medicine and DentistryTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Department of Pathological Biochemistry, Medical Research InstituteTokyo Medical and Dental UniversityTokyoJapan
  3. 3.Department of General Medicine, Graduate School of Medicine and DentistryTokyo Medical and Dental UniversityTokyoJapan

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