Photobiomodulation effects on head and neck squamous cell carcinoma (HNSCC) in an orthotopic animal model
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Photobiomodulation (PBM) has shown efficacy in preventing and treating cancer therapy-induced mucositis and dermatitis. However, there is contradictory information regarding the effect of PBM on (pre)malignant cells, which has led to questions regarding the safety of this technique. We address this issue using an orthotopic mouse model (Cal-33) with human squamous cell carcinoma of the oral cavity.
Mice with actively growing orthotopic Cal-33 head and neck carcinoma tumors were divided into 4 groups: control, PBM only, radiation therapy (RT) only, and PBM + RT. We performed three experiments: (1) PBM at 660 nm, 18.4 J/cm2, and 5 RT × 4 Gy doses delivered daily; (2) PBM at 660 nm, 18.4 J/cm2, and 1 × 15 Gy RT; and (3) PBM at 660 nm + 850 nm, 45 mW/cm2, 3.4 J/cm2, and 1 × 15 Gy RT. Mice were weighed daily and tumor volumes were evaluated by IVIS. Survival time was also evaluated.
Animals treated with RT survived significantly longer and had significantly smaller tumor volume when compared with the control and PBM-only treatment groups. No significant differences were noted between the RT alone and PBM + RT groups in any of the experiments.
Our results suggest that PBM at the utilized parameters does not provide protection to the tumor from the killing effects of RT.
KeywordsPhotobiomodulation Radiation therapy Head and neck cancer Orthotopic mouse model
We thank the following core facilities at MSKCC: Molecular Cytology and Small Animal Imaging.
This work was supported in part by the Department of Radiation Oncology and the Cancer Center Support Grant (P30 CA008748), the NIH.
Compliance with ethical standards
Conflict of interest
James Carrol is the CEO of Thor Laser. None of the other authors declare any conflict of interest.
- 7.Lalla RV, Bowen J, Barasch A, Elting L, Epstein J, Keefe DM, McGuire DB, Migliorati C, Nicolatou-Galitis O, Peterson DE, Raber-Durlacher JE, Sonis ST, Elad S, The Mucositis Guidelines Leadership Group of the Multinational Association of Supportive Care in Cancer and International Society of Oral Oncology (MASCC/ISOO) (2014) MASCC/ISOO clinical practice guidelines for the management of mucositis secondary to cancer therapy. Cancer 120:1453–1461CrossRefGoogle Scholar
- 9.Clarkson JE, Worthington HV, Furness S et al (2010) Interventions for treating mucositis for patients with cancer receiving treatment. Cochrane Database Syst Rev 8:CD001973Google Scholar
- 12.Rhee YH, Moon JH, Choi SH, Ahn JC (2016) Low-level laser therapy promoted aggressive proliferation and angiogenesis through decreasing of transforming growth factor-b1 and increasing of Akt/Hypoxia inducible factor -1a in anaplastic thyroid cancer. Photomed Laser Surg 34:229–235CrossRefGoogle Scholar
- 15.Djavid GE, Bigdeli B, Goliaei B, Nikoofar A, Hamblin MR (2017) Photobiomodulation leads to enhanced radiosensitivity through induction of apoptosis and autophagy in human cervical cancer cells. J Biophotonics 10:1732–1742. https://doi.org/10.1002/jbio.201700004 CrossRefPubMedPubMedCentralGoogle Scholar
- 17.Antunes HS, Herchenhorn D, Small IA, Araújo CMM, Viégas CMP, de Assis Ramos G, Dias FL, Ferreira CG (2017) Long-term survival of a randomized phase III trial of head and neck cancer patients receiving concurrent chemoradiation therapy with or without low-level laser therapy (LLLT) to prevent oral mucositis. Oral Oncol 71:11–15CrossRefGoogle Scholar
- 18.Mizrachi A, Vinagolu RK, Brook S, Ghossein R, Haimovitz Friedman A (2018) A novel orthotopic oral squamous cell carcinoma animal model for preclinical studies of different treatment modalities. JAMA (abstr)Google Scholar
- 19.Ottaviani G, Martinelli V, Rupel K, Caronni N, Naseem A, Zandonà L, Perinetti G, Gobbo M, di Lenarda R, Bussani R, Benvenuti F, Giacca M, Biasotto M, Zacchigna S (2016) Laser therapy inhibits tumor growth in mice by promoting immune surveillance and vessel normalization. EBioMedicine 11:165–172. https://doi.org/10.1016/j.ebiom.2016.07.028 CrossRefPubMedPubMedCentralGoogle Scholar
- 23.Zacchigna S, Gobbo M, Rupel K et al (2015) Is laser biostimulation safe even when performed in neoplastic fields? J Clin Oncol 33(suppl):3Google Scholar