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Wiener klinische Wochenschrift

, Volume 119, Issue 17–18, pp 534–537 | Cite as

Detection and identification of Borrelia burgdorferi sensu lato genospecies in ticks from three different regions in Slovakia

  • Katarína Smetanová
  • Caroline Burri
  • David Pérez
  • Lise Gern
  • Elena KocianováEmail author
Original Article

Summary

Lyme borreliosis is one of the most common tick-borne diseases that occur in Slovakia. In this study, Borrelia burgdorferi sensu lato was detected and cultivated from questing ticks collected in three areas of Slovakia. Two methods, restriction fragment length polymorphism and reverse line blot, were used for identification of isolates and determination of the prevalence of B. burgdorferi s.l. in the ticks. The prevalence of B. burgdorferi s.l. in I. ricinus detected by reverse line blot was 31.9%. Four genospecies, namely B. garinii, B. valaisiana, B. afzelii and B. burgdorferi sensu stricto were found. B. garinii was the most prevalent genospecies.

Keywords

Borrelia burgdorferi Tick Ixodes ricinus Slovakia Reverse line blot 

Nachweis und Identifizierung von Borrelia burgdorferi sensu lato-Genospezies in Zecken aus drei verschiedenen Regionen der Slowakei

Zusammenfassung

Lyme-Borreliose ist eine der häufigsten von Zecken vermittelten Erkrankungen, die in der Slowakei auftreten. In dieser Studie wurde Borrelia burgdorferi sensu lato in Wirt-suchenden Zecken, die aus drei Regionen der Slowakei gesammelt worden sind, nachgewiesen und kultiviert. Zur Identifizierung der Genospezies wurden zwei Methoden eingesetzt, die RFLP (restriction fragment length polymorphism) und der "reverse line blot". Ebenso wurde die Prävalenz von B. burgdorferi s.l. in den Zecken ermittelt, welche rund 32% betrug. Von den vier identifizierten Genospezies, B. afzelii, B. burgdorferi sensu stricto, B. garinii und B. valaisiana, wurde B. garinii am häufigsten nachgewiesen.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Katarína Smetanová
    • 2
  • Caroline Burri
    • 1
  • David Pérez
    • 1
  • Lise Gern
    • 1
  • Elena Kocianová
    • 2
    Email author
  1. 1.Institute of BiologyUniversity of NeuchâtelNeuchâtelSwitzerland
  2. 2.Institute of VirologySlovak Academy of SciencesBratislavaSlovakia

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