Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis
- Cite this article as:
- Ding, Z., Wang, T., Chong, K. et al. Sex Plant Reprod (2001) 13: 285. doi:10.1007/s004970100065
Yeast DMC1 is a meiosis-specific gene required for homologous chromosome pairing in meiosis. Using degenerate primers designed according to amino acid motifs conserved in yeast Dmc1 and Arabidopsis AtDmc1, we obtained full-length cDNA of a rice homologue of the DMC1 gene (OsDMC1) by RT-PCR and rapid amplification of cDNA ends (RACEs). OsDmc1 exhibited 53% amino acid sequence identity to yeast Dmc1 and 81% to AtDmc1. OsDMC1 was expressed at high-levels in reproductive organs, low-levels in roots, and undetectable levels in leaves and seedlings. Southern blot analyses revealed that OsDMC1 is one of two DMC1 homologues present in rice.
Unable to display preview. Download preview PDF.