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Sexual Plant Reproduction

, Volume 10, Issue 3, pp 173–180 | Cite as

Adhesion of lily pollen tubes on an artificial matrix

  • Guang Yuh Jauh
  • Kathleen J. Eckard
  • Eugene A. Nothnagel
  • E. M. Lord
ORIGINAL PAPER

Abstract

 We proposed that pollination in lily is a case of cell adhesion and cell movement, but experimental evidence for the adhesion event is lacking. In this study, we developed an artificial extracellular matrix that mimics the in vivo lily stylar transmitting tract. This artificial matrix was created by applying the transmitting tract exudate extracted from lily styles onto a nitrocellulose membrane. When in vitro-grown pollen tubes were applied to the matrix, they adhered by their tips to the area of the stylar exudate which is rich in arabinogalactan proteins. Once they adhered, they grew on the in vitro artificial matrix at rates faster than normal. This is the first experimental evidence demonstrating the adhesion of in vitro-grown pollen tubes, an event that has been described as common in vivo. The adhesion event is stylar exudate specific, concentration dependent, and is affected by the developmental age of the pollen tube. This bioassay for pollen tube adhesion will be used to isolate the adhesive molecules from the stylar exudate.

Key words Adhesion assay Artificial plant extracellular matrix Lilium longiflorum Thunb. Pollen tube Stylar exudate 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Guang Yuh Jauh
    • 1
  • Kathleen J. Eckard
    • 1
  • Eugene A. Nothnagel
    • 1
  • E. M. Lord
    • 1
  1. 1.Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA, Tel. 1-909-7874441; Fax +1-909-7874437 e-mail lord@citrus.ucr.eduUS

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