Advertisement

Sexual Plant Reproduction

, Volume 12, Issue 4, pp 241–244 | Cite as

A new procedure to asses pollen viability

  • T. Rodriguez-Riano
  • A. Dafni
Short Communication

Abstract 

We tested pollen viability of eight species using four vital dyes, a new peroxidase test together with three other established methods (MTT, Baker’s and X-Gal), to determine their potential to differentiate fresh pollen from pollen heated for 2 h and 24 h at 80°C (killed pollen) and compared the results with in vitro germination. We found that two of three dyes previously employed to determine viability also stained killed pollen, while the new peroxidase test and MTT did not. We suggest that the latter two are the best methods to test pollen viability, since they do not normally stain either killed or aborted pollen.

Key words Pollen germination MTT Peroxidase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • T. Rodriguez-Riano
    • 1
  • A. Dafni
    • 1
  1. 1.Institute of Evolution, Haifa University, Haifa 31905, Israel e-mail: adafni@research.haifa.ac.il Fax: +972-4-8240312IL

Personalised recommendations