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Regulation of floral meristem activity through the interaction of AGAMOUS, SUPERMAN, and CLAVATA3 in Arabidopsis

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Key message

Floral meristem size is redundantly controlled by CLAVATA3, AGAMOUS , and SUPERMAN in Arabidopsis.

Abstract

The proper regulation of floral meristem activity is key to the formation of optimally sized flowers with a fixed number of organs. In Arabidopsis thaliana, multiple regulators determine this activity. A small secreted peptide, CLAVATA3 (CLV3), functions as an important negative regulator of stem cell activity. Two transcription factors, AGAMOUS (AG) and SUPERMAN (SUP), act in different pathways to regulate the termination of floral meristem activity. Previous research has not addressed the genetic interactions among these three genes. Here, we quantified the floral developmental stage-specific phenotypic consequences of combining mutations of AG, SUP, and CLV3. Our detailed phenotypic and genetic analyses revealed that these three genes act in partially redundant pathways to coordinately modulate floral meristem sizes in a spatial and temporal manner. Analyses of the ag sup clv3 triple mutant, which developed a mass of undifferentiated cells in its flowers, allowed us to identify downstream targets of AG with roles in reproductive development and in the termination of floral meristem activity. Our study highlights the role of AG in repressing genes that are expressed in organ initial cells to control floral meristem activity.

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Acknowledgements

The authors would like to thank Akie Takahashi and Taeko Kawakami for technical assistance, and Elliot Meyerowitz for providing pWUS::GFP-ER lines. This work was supported by Grants from Japan Science and Technology Agency “Precursory Research for Embryonic Science and Technology (No. JPMJPR15QA),” a JSPS KAKENHI (No. 16H01468), the NAIST Foundation, the Sumitomo Foundation, the Takeda Foundation, and the Mishima Kaiun Memorial Foundation to N.Y.; a Grant from JSPS KAKENHI (No. 15H05955) to T. S.; a Grant from Japan Science and Technology Agency “Precursory Research for Embryonic Science and Technology (No. JPMJPR15Q2)” to Y.I.; Grants from JSPS KAKENHI (Nos. 15H05959 and 17H06172) to K.S.; and Grants from the NAIST Foundation, the Mitsubishi Foundation, and JSPS KAKENHI (15H01234, 15H01356, 15H02405, and 17H05843) to T.I.

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  1. Nobutoshi Yamaguchi and Yifeng Xu have contributed equally to this work.

Authors and Affiliations

  1. Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara, 630-0192, Japan

    Akira Uemura, Nobutoshi Yamaguchi & Toshiro Ito

  2. Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8, Honcho, Kawaguchi-shi, Saitama, 332-0012, Japan

    Nobutoshi Yamaguchi & Yasunori Ichihashi

  3. Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Republic of Singapore

    Yifeng Xu & WanYi Wee

  4. RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan

    Yasunori Ichihashi, Arisa Shibata & Ken Shirasu

  5. Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan

    Takamasa Suzuki

  6. Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo, 113-0033, Japan

    Ken Shirasu

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  1. Akira Uemura
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Correspondence to Toshiro Ito.

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Communicated by Tetsuya Higashiyama.

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Uemura, A., Yamaguchi, N., Xu, Y. et al. Regulation of floral meristem activity through the interaction of AGAMOUS, SUPERMAN, and CLAVATA3 in Arabidopsis . Plant Reprod 31, 89–105 (2018). https://doi.org/10.1007/s00497-017-0315-0

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