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Sexual Plant Reproduction

, Volume 19, Issue 4, pp 197–206 | Cite as

OsAP2-1, an AP2-like gene from Oryza sativa, is required for flower development and male fertility

  • Lifeng Zhao
  • Shengbao Xu
  • Tuanyao Chai
  • Tai WangEmail author
Original Article

Abstract

Arabidopsis AP2 (AtAP2) is a member of AP2 subfamily of the AP2/EREBP class of transcriptional factors and plays crucial roles in regulating floral meristem determinacy, floral organ identity and ovule and seed development. In order to examine functional conservation and diversification of AP2 genes in monocots, here we analyze a rice AtAP2-like gene, OsAP2-1. This gene was expressed at high levels in both flowers and roots, and at faint levels in buds or leaves. Its transcript was also present in mature pollen grains. The OsAP2-1 down-regulated lines mediated by RNA interference showed obvious alternation in floral organs of innermost two whorls, including the reduced stamens, fused anther filaments and increased number of pistils. Abortive seeds were observed in the down-regulated lines. These results suggest that OsAP2-1 is required for flower and seed development, consistent with the roles of AtAP2 in Arabidopsis. Furthermore, our results demonstrated that the down-regulation of the endogenous transcripts led to decreased pollen viability and germination activity, suggesting that OsAP2-1 has roles in controlling pollen development.

Keywords

OsAP2-1 RNA interference Flower development Pollen viability Oryza sativa 

Notes

Acknowledgments

The research was supported by grants from NSFC (No 30370138) and The Chinese Ministry of Sciences and Technology (Nos: 2005CB120802 and 2005CB120804).

References

  1. Boutilier K, Offringa R, Sharma VK, Kieft H, Ouellet T, Zhang L, Hattori J, Liu CM, van Lammeren AAM, Miki BLA, Custers JBM, van Lookeren Campagne MM (2002) Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. Plant Cell 14:1737–1749PubMedCrossRefGoogle Scholar
  2. Bowman JL, Meyerowitz EM (1991) Genetic control of pattern formation during flower development in Arabidopsis. Symp Soc Exp Biol 45:89–115PubMedGoogle Scholar
  3. Chuang CF, Meyerowitz EM (2000) Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci USA 97:4985–4990PubMedCrossRefGoogle Scholar
  4. Chuck G, Meeley RB, Hake S (1998) The control of maize spikelet meristem fate by the APETALA2-like gene indeterminate spikelet1. Genes Dev 12:1145–1154PubMedGoogle Scholar
  5. Ding ZJ, Wang T, Chong K, Bai SN (2001) Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis. Sex Plant Reprod 13:285–288CrossRefGoogle Scholar
  6. Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J 33:751–763PubMedCrossRefGoogle Scholar
  7. Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQJ, Gerentes D, Perez P, Smyth DR (1996) AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth. Plant Cell 8:155–168PubMedCrossRefGoogle Scholar
  8. Gutterson N, Reuber TL (2004) Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol 7:465–471PubMedCrossRefGoogle Scholar
  9. Haake V, Cook D, Riechmann JL, Pineda O, Thomashow MF, Zhang JZ (2002) Transcription factor CBF4 is a regulator of drought adaptation in Arabidopsis. Plant Physiol 130:639–648PubMedCrossRefGoogle Scholar
  10. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6:271–282PubMedCrossRefGoogle Scholar
  11. Jofuku KD, den Boer BGW, Van Montagu M, Okamuro JK (1994) Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell 6:1211–1225PubMedCrossRefGoogle Scholar
  12. Jofuku KD, Omidyar PK, Gee Z, Okamuro JK (2005) Control of seed mass and seed yield by the floral homeotic gene APETALA2. Proc Natl Acad Sci USA 102:3117–3122PubMedCrossRefGoogle Scholar
  13. Klucher KM, Chow H, Reiser L, Fischer RL (1996) The AINTEGUMENTA gene of Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2. Plant Cell 8:137–153PubMedCrossRefGoogle Scholar
  14. Kohli A, Twyman RM, Abranches R, Wegel E, Stoger E, Christou P (2003) Transgene integration, organization and interaction in plants. Plant Mol Biol 52:247–258PubMedCrossRefGoogle Scholar
  15. Kunst L, Klenz JE, Martinez-Zapater J, Haughn GW (1989) AP2 gene determines the identity of perianth organs in flowers of Arabidopsis thaliana. Plant Cell 1:1195–1208PubMedCrossRefGoogle Scholar
  16. Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki Y, Shinozaki K (1998) Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell 10:1391–1406PubMedCrossRefGoogle Scholar
  17. Mizukami Y, Fischer RL (2000) Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis. Proc Natl Acad Sci USA 97:942–947PubMedCrossRefGoogle Scholar
  18. Moose SP, Sisco PH (1996) Glossy15, an APETALA2-like gene from maize that regulates leaf epidermal cell identity. Genes Dev 10:3018–3027PubMedGoogle Scholar
  19. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325PubMedGoogle Scholar
  20. Ohto MA, Fischer RL, Goldberg RB, Nakamura K, Harada JJ (2005) Control of seed mass by APETALA2. Proc Natl Acad Sci USA 102:3123–3128PubMedCrossRefGoogle Scholar
  21. Okamuro JK, Caster B, Villarroel R, Van Montagu M, Jofuku DK (1997) The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc Natl Acad Sci USA 94:7076–7081PubMedCrossRefGoogle Scholar
  22. Rieu I, Bots M, Mariani C, Weterings KAP (2005) Isolation and expression analysis of a tobacco AINTEGUMENTA ortholog (NtANTL). Plant Cell Physiol 46:803–805PubMedCrossRefGoogle Scholar
  23. Wang SH, Chen F, Zhou KD (2000) In vitro germination of rice pollens. Acta Agronomica Sinica 26:609–611Google Scholar
  24. Weigel D (1995) The APETALA2 domain is related to a novel type of DNA binding domain. Plant Cell 7:388–389PubMedCrossRefGoogle Scholar
  25. Wesley SV, Helliwell CA, Smith NA, Wang M, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, Waterhouse PM (2001) Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J 27:581–590PubMedCrossRefGoogle Scholar
  26. Ye R, Yao QH, Xu ZH, Xue HW (2004) Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development. Plant J 38:348–357PubMedCrossRefGoogle Scholar
  27. Zhang L, Tao J, Wang S, Chong K, Wang T (2006) The rice OsRad21–4, an orthologue of yeast Rec8 protein, is required for efficient meiosis. Plant Mol Biol 60:533–554PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Lifeng Zhao
    • 1
    • 2
  • Shengbao Xu
    • 1
    • 2
  • Tuanyao Chai
    • 2
  • Tai Wang
    • 1
    Email author
  1. 1.Research Center for Molecular and Developmental Biology, Key Laboratory of Photosynthesis and Environmental Molecular PhysiologyInstitute of Botany, Chinese Academy of ScienceBeijingChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina

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