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Sexual Plant Reproduction

, Volume 16, Issue 2, pp 77–85 | Cite as

Transcript profiling on developing Petunia hybrida floral organs

  • Filip CnuddeEmail author
  • Chiaraluce Moretti
  • Andrea Porceddu
  • Mario Pezzotti
  • Tom Gerats
Original Article

Abstract

The cDNA-AFLP transcript profiling technique was used to analyse gene expression during flower development in Petunia hybrida. Reproductive and vegetative floral organs were sampled at five developmental stages and gene expression profiles were compared. This allowed us to assemble an inventory of genes expressed mainly in anthers during microspore development and in ovaries during macrosporogenesis. About 6,000 transcript tags were generated, 354 of which showed a modulated and/or organ-specific expression pattern. Stamen-specific transcripts exhibiting an upregulation in gene expression were well represented in our screening. Ovary-specific transcripts were less frequently observed and often displayed a constant level of gene expression. Of 194 fragments characterised further by sequencing, 35% showed homology with known genes in a database search. They belong to a wide range of gene classes, such as proteases, transcription factors and genes involved in metabolism, cell cycle and disease resistance. The usefulness of cDNA-AFLP transcript profiling as a tool to unravel complex developmental processes at the molecular level is discussed.

Keywords

Sporogenesis cDNA-AFLP Flower development Expression analysis Petunia 

Notes

Acknowledgements

Filip Cnudde was funded partly by the Flanders Fund for Scientific Research (FWO), partly by the Flemish Institute for Biotechnology (VIB) and partly by a grant from the subfaculty of Biology, University of Nijmegen.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Filip Cnudde
    • 1
    Email author
  • Chiaraluce Moretti
    • 2
  • Andrea Porceddu
    • 3
  • Mario Pezzotti
    • 4
  • Tom Gerats
    • 1
  1. 1.Department of Experimental BotanyUniversity of NijmegenNijmegenThe Netherlands
  2. 2.Dipartimento di Biologia Vegetale e Biotecnologie AgroambientaliUniversità degli Studi di PerugiaPerugiaItaly
  3. 3.Institute of Plant Genetics—CNR sez PerugiaPerugiaItaly
  4. 4.Dipartimento Scientifico e TecnologicoUniversità degli Studi di VeronaVeronaItaly

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