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Short-term heat shock proteins 70 and 90 mRNA expression profile and its relation to thermo-physiological parameters in goats exposed to heat stress

  • M. F. El-ZareiEmail author
  • A. M. Alseaf
  • A. A. Alhaidary
  • E. F. Mousa
  • A. B. Okab
  • E. M. Samara
  • K. A. Abdoun
Original Paper

Abstract

To follow the inheritance potential for heat tolerance after a crossing program in goats, 24 kids from four groups of goat kids (6 kids in each) from Aradi (A) and Damascus (D), their crossbred first-generation F1 (½D½A) and second-generation F2 (¾D¼A), were exposed to acute elevated environmental temperature in controlled climatic chambers. Rectal temperature (RT), respiratory rate (RR), heat tolerance coefficient (HTC), adaptability coefficient (AC), and mRNA expression of heat shock proteins 70 and 90 ((HSP70 and HASP90, respectively), using real-time PCR were estimated. Results showed that Aradi breed goats had the highest level of expression for heat shock proteins 70 and 90, followed by F1, F2, and Damascus (P ≤ 0.01). Crossbreeds and Damascus showed the highest RT, while Aradi breed showed the lowest value (P ≤ 0.01). Aradi and crossbreeds showed the highest RR, while Damascus showed the lowest RR (P ≤ 0.05). Aradi and F1 showed the highest HTC (P ≤ 0.05), while there was no significant difference between pure breeds and crossbreeds in AC. A significant positive phenotypic correlation (0.81) was observed between HSP70 and HSP90. In addition, RR showed moderate positive correlation with both HSP70 and HSP90. It could be concluded that Aradi breed had putative heat tolerance in comparison to its crossbred progeny from the Damascus breed. The crossbreeding may result in some loss of heat tolerance potential, but the crossbreeds still better adapted to high environmental temperature than the Damascus breed.

Keywords

Adaptability coefficient Heat tolerance coefficient Rectal temperature Respiratory rate Crossbred 

Notes

Funding information

This Project was funded by the National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia,

Award Number (12-AGR2540-02).

Compliance with ethical standards

The protocol of animal experiments was approved by the Faculty Research Ethics Committee, King Saud University, Saudi Arabia, where the practical experiment was done.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© ISB 2019

Authors and Affiliations

  • M. F. El-Zarei
    • 1
    • 2
    Email author
  • A. M. Alseaf
    • 1
  • A. A. Alhaidary
    • 3
  • E. F. Mousa
    • 1
    • 4
  • A. B. Okab
    • 3
    • 5
  • E. M. Samara
    • 3
  • K. A. Abdoun
    • 3
  1. 1.Department of Animal Production and Breeding, College of Agriculture and Veterinary MedicineQassim UniversityBuraydahSaudi Arabia
  2. 2.Department of Animal Production, Faculty of AgricultureSuez Canal UniversityIsmailiaEgypt
  3. 3.Department of Animal Production, College of Food and Agriculture SciencesKing Saud UniversityRiyadhSaudi Arabia
  4. 4.Department of Animal Production, Faculty of AgricultureAssiut UniversityAssiutEgypt
  5. 5.Department of Environmental Studies, Institute of Graduate Studies and ResearchAlexandria UniversityAlexandriaEgypt

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