Effect of heat stress on protein utilization and nutrient transporters in meat-type chickens
The aim of this study was to investigate the effect of heat stress (HS) on digestibility of protein and fat and the expression of nutrient transporters in broilers. Forty-eight male Cobb500 chicks were used in this study. At day 14, birds were randomly divided into two groups and kept under either constant normal temperature (25 °C) or high temperature (35 °C) in individual cages. Five birds per treatment at 1 and 12 days post-treatment were euthanized, and Pectoralis major (P. major) and ileum were sampled for gene expression analysis. At day 33, ileal contents were collected and used for digestibility analysis. The total consumption and retention of protein and fat were significantly lower in the HS group compared to the control group. Meanwhile, the retention of crude protein per BWG was significantly higher in the HS group compared to the control group. In P. major and ileum tissues at day 1, transporters FATP1 and SGLT1 were down-regulated in the HS group. Meanwhile, FABP1 and PepT1 were down-regulated only in the ileum of the HS group. The converse was shown in P. major. The nutrient transporter FABP1 at day 12 post-HS was down-regulated in the P. major and ileum, but GLUT1 and PepT2 were down-regulated only in the ileum, and PepT1 was down-regulated only in the P. major compared with the control group. These changes in nutrient transporters suggest that high ambient temperature might change the ileum and P. major lipids, glucose, and oligopeptide transporters.
KeywordsFeed intake Nutrient transporters Digestibility Pectoralis major
Walid Habashy was supported by the Missions sector of the Egyptian Ministry of Higher Education.
Compliance with ethical standards
Human and animal rights
Research on live chickens met the guidelines approved by the institutional animal care and use committee of the University of Georgia.
- AOAC (2000) Official methods of analysis, 17th edn. Association of Official Analytical Chemists, Washington, DCGoogle Scholar
- Attia YA, Hassan RA, Tag El-Din AE, Abou-Shehema BM (2011) Effect of ascorbic acid or increasing metabolizable energy level with or without supplementation of some essential amino acids on productive and physiological traits of slow-growing chicks exposed to chronic heat stress. J Anim Physiol Anim Nutr 95:744–755CrossRefGoogle Scholar
- de Sousa AR, Penalva LO, Marcotte EM, Vogel C (2009) Global signatures of protein and mRNA expression levels. Mol Bio Syst 5:1512–1526Google Scholar
- Ferrer C, Pedragosa E, Torras-Llort M, Parcerisa X, Rafecas M, Ferrer R, Amat C, Moretó M (2003) Dietary lipids modify brush border membrane composition and nutrient transport in chicken small intestine. J Nutr 133:1147–1153Google Scholar
- Geraert PA, Padilha JCF, Guillaumin S (1996) Metabolic and endocrine changes induced by chronic heat exposure in broiler chickens: growth performance, body composition and energy retention. Br J Nutr 75:195–204Google Scholar
- Gorboulev V, Schürmann A, Vallon V, Kipp H, Jaschke A, Klessen D, Friedrich A, Scherneck S, Rieg T, Cunard R, Veyhl-Wichmann M, Srinivasan A, Balen D, Breljak D, Rexhepaj R, Parker HE, Gribble FM, Reimann F, Lang F, Wiese S, Sabolic I, Sendtner M, Koepsell H (2012) Na+-D-glucose cotransporter SGLT1 is pivotal for intestinal glucose absorption and glucose-dependent incretin secretion. Diabetes 61:187–196CrossRefGoogle Scholar
- McArthur MJ, Atshaves BP, Frolov A, Foxworth WD, Kier AB, Schroeder F (1999) Cellular uptake and intracellular trafficking of long chain fatty acids. J Lipid Res 40:1371–1383Google Scholar
- Munro HN, Fleck A (1969) Analysis of tissues and body fluids for nitrogenous constituents. In: Munro HD (ed) Mammalian protein metabolism, vol Vol III. Academic Press, New York, pp 424–525Google Scholar
- SAS Institute (2011) SAS/SAT user guide: statistics ver. 9.3 edn. SAS Institute Inc., CaryGoogle Scholar
- Song Z, Liu L, Sheikhahmadi A, Jiao H, Lin H (2012) Effect of heat exposure on gene expression of feed intake regulatory peptides in laying hens. J Biomed Biotechnol. doi: 10.1155/2012/484869
- Steel A, Hediger MA (1998) The molecular physiology of sodium and proton-coupled solute transporters. News Physiol Sci 13:123–131Google Scholar
- Temim S, Chagneau AM, Peresson R, Tesseraud S (2000) Chronic heat exposure alters protein turnover of three different skeletal muscles in finishing broiler chickens fed 20 or 25% protein diets. J Nutr 130:813–819Google Scholar