Biological effects of the electrostatic field: red blood cell-related alterations of oxidative processes in blood
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The aim of this study was to determine activities of pro-/antioxidant enzymes, reactive oxygen species (ROS) content, and oxidative modification of proteins and lipids in red blood cells (RBCs) and blood plasma of rats exposed to electrostatic field (200 kV/m) during the short (1 h) and the long periods (6 day, 6 h daily). Short-term exposure was characterized by the increase of oxidatively damaged proteins in blood of rats. This was strongly expressed in RBC membranes. After long-term action, RBC content in peripheral blood was higher than in control (P < 0.01) and the attenuation of prooxidant processes was shown.
External electrostatic field (200 kV/m) alters the balance in pro-/antioxidant processes.
We examine oxidative processes in plasma and RBC (hemolysate and membranes).
Biological effects of static electric field depend on exposure time.
Acute action of electrostatic field (ESF) characterized by activation of the prooxidant processes.
Long-term exposure reflected with prevalence of antioxidant activities.
KeywordsCarbonylation of proteins Electrostatic field Oxidative processes Reactive oxygen species Red blood cells
The research is supported by SCS MES RA, within the frames of joint Armenian—Belarusian research project No. 13РБ-047.
Conflict of interest
The authors declare that they have no competing interests.
- Abugo OO, Rifkind JM (1994) Oxidation of hemoglobin and the enhancement produced by nitroblue tetrazolium. J Biol Chem 269:24845–24853Google Scholar
- Advisory group on non-ionizing radiation (1994) Health effects related to the use of visual display units, Report of Advisory Group on Non-Ionising Radiation, Doc NRPB 5(2).Google Scholar
- Antipov VV, Dobrov NN, Drobyshev VI, Koroleva LV, Nikitin MD (1983) Biological effects of the action of a high-tension DC electrical field. Kosm Biol Aviakosm Med 17:50–54Google Scholar
- Artsruni GG, Zil'fian AV, Azgaldian NR, Dovlatian RA (1987) Effect of an external electrostatic field on catecholamine secretion by rat adrenals. Kosm Biol Aviakosm Med 21:67–70Google Scholar
- Artsruni GG, Batikyan TB, Tadevosyan YV (1999) Influence of external electrostatic fields on enzyme systems of phospholipid deacylation. Biochemistry 64:1279–1282Google Scholar
- Beers RF Jr, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140Google Scholar
- Cieślar G, Sieroń A, Sowa P (2003) Influence of high voltage static electric field on activity of antioxidant enzymes in rats. In Engineering in Medicine and Biology Society ‘2003 Proceedings of 25th Annual International Conference of the IEEE 4:3257–3260Google Scholar
- European Commission (1996) Non-ionizing radiation—sources, exposure and health effects. Office for Official Publications of the European Communities, LuxemburgGoogle Scholar
- Halliwell B, Gutteridge JMC (1989) Eds. Lipid peroxidation: a radical chain reaction. In: Free radicals in biology and medicine. (2nd ed., p. 188) Oxford: Clarendon Press.Google Scholar
- Harutyunyan H, Khachatryan L, Soghomonyan A, Artsruni G (2013) Modification of oxidative processes in blood by the external static electric field. New Arm Med J 7(1):22–32Google Scholar
- Kostyuk VA, Potapovich AI (1989) Superoxide-driven oxidation of quercetin and a simple assay for determination of superoxide dismutase. Biochem Int 19:1117–1124Google Scholar
- Levine RL, Garland D, Oliver CN, Amici A, Climent L, Leny AG et al (1990) Determination of carbonyl content in oxidatively modified proteins. In: Packer L, Glazer AN (eds) Methods in enzymology, 186th edn, Oxygen radicals in biological systems part B: oxygen radicals and antioxidants. Academic, San Diego, pp 464–478Google Scholar
- Ohta S (1985) The effects of DC high electric field exposure upon sensory receptors of cat’s hindlimb. Hokkaido Igaky Zasshi 60:713–723Google Scholar
- Reusch VM, Burger MM (1974) Distribution of marker enzymes between mesosomal and protoplast membranes. J Biol Chem 249:5337–5345Google Scholar
- Rifkind JM, Abugo O (1994) Alterations in erythrocyte deformability under hypoxia: implications for impaired oxygen transport. In: Hogan MC, Mathieu-Costello O, Poole DC, Wagner PD (eds) Oxygen transport to tissue, vol 16. Plenum Press, New York, pp 345–351Google Scholar
- Rifkind JM, Zhang L, Heim JM, Levy A (1988) The role of hemoglobin in generating oxyradicals. Basic Life Sci 49:157–162Google Scholar
- Van der Vliet A, Hu ML, O'Neill CA, Kaur H, Darley-Usmar V, Cross CE (1994) Interactions of human blood plasma with hydrogen peroxide and hypochlorous acid. J Lab Clin Med 124(5):701–707Google Scholar