International Journal of Biometeorology

, Volume 60, Issue 1, pp 99–111 | Cite as

Biological effects of the electrostatic field: red blood cell-related alterations of oxidative processes in blood

Original Paper

Abstract

The aim of this study was to determine activities of pro-/antioxidant enzymes, reactive oxygen species (ROS) content, and oxidative modification of proteins and lipids in red blood cells (RBCs) and blood plasma of rats exposed to electrostatic field (200 kV/m) during the short (1 h) and the long periods (6 day, 6 h daily). Short-term exposure was characterized by the increase of oxidatively damaged proteins in blood of rats. This was strongly expressed in RBC membranes. After long-term action, RBC content in peripheral blood was higher than in control (P < 0.01) and the attenuation of prooxidant processes was shown.

Highlights

  • External electrostatic field (200 kV/m) alters the balance in pro-/antioxidant processes.

  • We examine oxidative processes in plasma and RBC (hemolysate and membranes).

  • Biological effects of static electric field depend on exposure time.

  • Acute action of electrostatic field (ESF) characterized by activation of the prooxidant processes.

  • Long-term exposure reflected with prevalence of antioxidant activities.

Keywords

Carbonylation of proteins Electrostatic field Oxidative processes Reactive oxygen species Red blood cells 

Notes

Acknowledgments

The research is supported by SCS MES RA, within the frames of joint Armenian—Belarusian research project No. 13РБ-047.

Conflict of interest

The authors declare that they have no competing interests.

References

  1. Abugo OO, Rifkind JM (1994) Oxidation of hemoglobin and the enhancement produced by nitroblue tetrazolium. J Biol Chem 269:24845–24853Google Scholar
  2. Advisory group on non-ionizing radiation (1994) Health effects related to the use of visual display units, Report of Advisory Group on Non-Ionising Radiation, Doc NRPB 5(2).Google Scholar
  3. Alcaraz M, Olmos E, Alcaraz-Saura M, Achel DG, Castillo J (2014) Effect of long-term 50 Hz magnetic field exposure on the micronucleated polychromatic erythrocytes of mice. Electromagn Biol Med 33(1):51–57CrossRefGoogle Scholar
  4. Alvarez JG, Storey BT (1995) Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev 42:334–346CrossRefGoogle Scholar
  5. Antipov VV, Dobrov NN, Drobyshev VI, Koroleva LV, Nikitin MD (1983) Biological effects of the action of a high-tension DC electrical field. Kosm Biol Aviakosm Med 17:50–54Google Scholar
  6. Artsruni GG, Zil'fian AV, Azgaldian NR, Dovlatian RA (1987) Effect of an external electrostatic field on catecholamine secretion by rat adrenals. Kosm Biol Aviakosm Med 21:67–70Google Scholar
  7. Artsruni GG, Batikyan TB, Tadevosyan YV (1999) Influence of external electrostatic fields on enzyme systems of phospholipid deacylation. Biochemistry 64:1279–1282Google Scholar
  8. Artsruni GG, Sahakyan GV, Poghosyan GA (2013) In vitro effect of the external electrostatic field on biophysical parameters of erythrocytes membranes. Biophysics 58(6):1022–1027CrossRefGoogle Scholar
  9. Asha Devi S, Shiva Shankar Reddy CS, Subramanyam MV (2009) Oxidative stress and intracellular pH in the young and old erythrocytes of rat. Biogerontology 10(6):659–669CrossRefGoogle Scholar
  10. Ballou D, Palmer G, Massey V (1969) Direct demonstration of superoxide anion production during the oxidation of reduced flavin and of its catalytic decomposition by erythrocuprein. Biochem Biophys Res Commun 36:898–904CrossRefGoogle Scholar
  11. Beers RF Jr, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140Google Scholar
  12. Cieślar G, Sieroń A, Sowa P (2003) Influence of high voltage static electric field on activity of antioxidant enzymes in rats. In Engineering in Medicine and Biology Society ‘2003 Proceedings of 25th Annual International Conference of the IEEE 4:3257–3260Google Scholar
  13. Cieslar G, Malyszek-Tumidajewicz J, Sowa P, Sieron A (2012) Impact of static electric field on prooxidant-antioxidant balance in rats. COMPEL: The International Journal for Computation and Mathematics in Electrical and Electronic Engineering 31(4):1212–1223CrossRefGoogle Scholar
  14. Dalle-Donne I, Rossi R, Giustarini D, Colombo R, Milzani A (2003) Protein carbonyl groups as biomarkers of oxidative stress. Clin Chim Acta 329:23–28CrossRefGoogle Scholar
  15. DeBruin KA, Krassowska W (1999) Modeling electroporation in a single cell. I. Effects of field strength and rest potential. Biophys J 77(3):1213–1224CrossRefGoogle Scholar
  16. Dodge JT, Mitchell C, Hanahan D (1963) The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys 100(1):119–130CrossRefGoogle Scholar
  17. European Commission (1996) Non-ionizing radiation—sources, exposure and health effects. Office for Official Publications of the European Communities, LuxemburgGoogle Scholar
  18. Halliwell B, Gutteridge JMC (1989) Eds. Lipid peroxidation: a radical chain reaction. In: Free radicals in biology and medicine. (2nd ed., p. 188) Oxford: Clarendon Press.Google Scholar
  19. Halliwell B, Grootveld M, Gutteridge JMC (1988) Methods for the measurement of hydroxyl radicals in biochemical systems: deoxyribose degradation and aromatic hydroxylation. Methods Biochem Anal 33:59–90CrossRefGoogle Scholar
  20. Harutyunyan HA, Artsruni GG (2013) Biological effects of static electric field: plasma/serum proteome analysis of rats. Electromagnetic Biology and Medicine 32(1):79–94CrossRefGoogle Scholar
  21. Harutyunyan H, Khachatryan L, Soghomonyan A, Artsruni G (2013) Modification of oxidative processes in blood by the external static electric field. New Arm Med J 7(1):22–32Google Scholar
  22. Kiefmann R, Rifkind JM, Nagababu E, Bhattacharya J (2008) Red blood cells induce hypoxic lung inflammation. Blood 11:5205–5214CrossRefGoogle Scholar
  23. Kostyuk VA, Potapovich AI (1989) Superoxide-driven oxidation of quercetin and a simple assay for determination of superoxide dismutase. Biochem Int 19:1117–1124Google Scholar
  24. Levine RL, Garland D, Oliver CN, Amici A, Climent L, Leny AG et al (1990) Determination of carbonyl content in oxidatively modified proteins. In: Packer L, Glazer AN (eds) Methods in enzymology, 186th edn, Oxygen radicals in biological systems part B: oxygen radicals and antioxidants. Academic, San Diego, pp 464–478Google Scholar
  25. Lott JR, McCain HB (1973) Some effects of continuous and pulsating electric fields on brain wave activity in rats. Int J Biometeorol 17:221–225CrossRefGoogle Scholar
  26. Marino AA, Berger TJ, Mitchell JT, Duhacek BA, Becker RO (1974) Electric field effects in selected biologic systems. Ann N Y Acad Sci 238:436–443CrossRefGoogle Scholar
  27. Morre DJ, Brightman AO (1991) NADH oxidase of plasma membranes. J Bioenerg Biomembr 23(4):469–489CrossRefGoogle Scholar
  28. Ohta S (1985) The effects of DC high electric field exposure upon sensory receptors of cat’s hindlimb. Hokkaido Igaky Zasshi 60:713–723Google Scholar
  29. Pick A, Keisari Y (1981) Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages. Cell Immunol 59:301–308CrossRefGoogle Scholar
  30. Poniedzialek B, Rzymski P, Nawrocka-Bogusz H, Jaroszyk F, Wiktorowicz K (2013) The effect of electromagnetic field on reactive oxygen species production in human neutrophils in vitro. Electromagnetic Biology and Medicine 32(3):333–341CrossRefGoogle Scholar
  31. Prütz WA, Butler J, Land EJ (1983) Phenol coupling initiated by one-electron oxidation of tyrosine units in peptides and histone. Int J Radiat Biol 44:183–196CrossRefGoogle Scholar
  32. Reusch VM, Burger MM (1974) Distribution of marker enzymes between mesosomal and protoplast membranes. J Biol Chem 249:5337–5345Google Scholar
  33. Rifkind JM, Abugo O (1994) Alterations in erythrocyte deformability under hypoxia: implications for impaired oxygen transport. In: Hogan MC, Mathieu-Costello O, Poole DC, Wagner PD (eds) Oxygen transport to tissue, vol 16. Plenum Press, New York, pp 345–351Google Scholar
  34. Rifkind JM, Zhang L, Heim JM, Levy A (1988) The role of hemoglobin in generating oxyradicals. Basic Life Sci 49:157–162Google Scholar
  35. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG (1973) Selenium: biochemical roles as a component of glutathione peroxidase. Science 179(73):588–590CrossRefGoogle Scholar
  36. Seyhan N, Güler G (2006) Review of in vivo static and ELF electric fields studies performed at Gazi biophysics department. Electromagnetic Biology and Medicine 25(4):307–323CrossRefGoogle Scholar
  37. Teale FWJ (1960) The ultraviolet fluorescence of proteins in neutral solution. Biochem J 76:381–388CrossRefGoogle Scholar
  38. Uchiyama M, Mihara M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271–278CrossRefGoogle Scholar
  39. Van der Vliet A, Hu ML, O'Neill CA, Kaur H, Darley-Usmar V, Cross CE (1994) Interactions of human blood plasma with hydrogen peroxide and hypochlorous acid. J Lab Clin Med 124(5):701–707Google Scholar
  40. Witko V, Nguyen AT, Descamps-Latscha B (1992) Microtiter plate assay for phagocyte derived taurine-chloramines. J Clin Lab Anal 6:47–53CrossRefGoogle Scholar

Copyright information

© ISB 2015

Authors and Affiliations

  1. 1.Laboratory of Biochemical and Biophysical Investigations, Scientific-Research CenterYerevan State Medical University after M. HeratsiYerevanArmenia

Personalised recommendations