International Journal of Biometeorology

, Volume 54, Issue 6, pp 653–661 | Cite as

Adaptive capability as indicated by endocrine and biochemical responses of Malpura ewes subjected to combined stresses (thermal and nutritional) in a semi-arid tropical environment

  • Veerasamy Sejian
  • Vijai P. Maurya
  • Sayeed M. K. Naqvi
Original Paper

Abstract

A study was conducted to assess the effect of combined stresses (thermal and nutritional) on endocrine and biochemical responses in Malpura ewes. Twenty eight adult Malpura ewes (average body weight 33.56 kg) were used in the present study. The ewes were divided into four groups viz., GI (n = 7; control), GII (n = 7; thermal stress), GIII (n = 7; nutritional stress) and GIV (n = 7; combined stress). The animals were stall fed with a diet consisting of 60% roughage and 40% concentrate. GI and GII ewes were provided with ad libitum feeding while GIII and GIV ewes were provided with restricted feed (30% intake of GI ewes) to induce nutritional stress. GII and GIV ewes were kept in climatic chamber at 40°C and 55% RH for 6 h a day between 1000 hours and 1600 hours to induce thermal stress. The study was conducted for a period of two estrus cycles. The parameters studied were Hb, PCV, glucose, total protein, total cholesterol, ACP, ALP, cortisol, T4, T3, and insulin. Combined stress significantly (P < 0.05) affected all parameters studied. Furthermore, the results revealed that, compared to thermal stress, nutritional stress had a less significant effect on the parameters studied. However, when both these stresses were coupled, they had a severe impact on all the parameters studied in these ewes. It can be concluded from this study that two stressors occurring simultaneously may impact severely on the biological functions necessary to maintain homeostasis in sheep.

Keywords

Malpura ewe Thermal stress Nutritional stress Endocrine response Biochemical parameter 

References

  1. Abecia JA, Zuniga O, Forcada F (2001) Effect of melatonin treatment in spring and feed intake on wool growth and thyroxine secretion in Rasa Aragonesa ewes. Small Rumin Res 41:265–270CrossRefGoogle Scholar
  2. Abou-Zeina HAA, Hassan HG, Sabra HA, Hamam AM (2009) Trials for elevating adverse effect of heat stress in buffaloes with emphasis on metabolic status and fertility. Global Veterinaria 3(1):51–62Google Scholar
  3. Al-Haidary AA (2004) Physiological responses of Naimey Sheep to heat stress challenge under semi-arid environments. Int J Agr Biol 2:307–309Google Scholar
  4. Ali A, Hayder M (2008) Seasonal variation of reproductive performance, foetal development and progesterone concentrations of sheep in the subtropics. Reprod Domest Anim 43(6):730–734. doi:10.1111/j.1439-0531.2007.00980.x
  5. Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC (1974) Enzymatic determination of total serum cholesterol. Clin Chem 20:470–475Google Scholar
  6. Alvarez MB, Johnson HD (1973) Environmental heat exposure on cattle plasma catecholamines and glucocorticoids. J Dairy Sci 56:189–194CrossRefGoogle Scholar
  7. Amstalden M, Garcia MR, Williams SW, Stanko RL, Nizielski SE, Morrison CD, Keisler DH, Williams GL (2000) Leptin gene expression, circulating leptin, and luteinizing hormone pulsatility are acutely responsive to short-term fasting in prepubertal heifers: relationships to circulating insulin and insulin-like growth factor I. Biol Reprod 63:127–133CrossRefGoogle Scholar
  8. Ashutosh, Dhanda OP, Kundu RL (2001) Eject of climate on the seasonal endocrine profile of native and crossbred sheep under semi-arid conditions. Trop Anim Health Prod 33:241–252CrossRefGoogle Scholar
  9. Balasubramaniam P, Malathi A (1992) Comparative study of hemoglobin estimated by Drabkin’s and Sahli’s methods. J Postgrad Med 38:8–9Google Scholar
  10. Broucek J, Kisac P, Uhrincat M (2009) Effect of hot temperatures on the hematological parameters, health and performance of calves. Int J Biometeorol 53:201–208CrossRefGoogle Scholar
  11. Brown-Brandl TM, Nienaber JA, Eigenberg RA, Hahn GL, Freetly H (2003) Thermoregulatory responses of feeder cattle. J Therm Biol 28:149–157CrossRefGoogle Scholar
  12. Daader AH, Marai, IFM, Habeeb AA, Yousef HM (1989) Improvement of growth performance of Friesian calves under Egyptian sub-tropical conditions. 1. Internal cooling technique using diuretics and drinking cool water. In: Proceedings of the 3rd Egyptian British Conference on Animal, Fish and Poultry Production, Vol. 2, Alexandria, pp 595–605Google Scholar
  13. Dickson WM (1993) Endocrine glands. In: Swenson MJ, Reece WO (eds) Dukes Physiology of domestic animal, 11th edn. Comstock, Ithaca, pp 629–664Google Scholar
  14. Daramola JO, Adeloye AA (2009) Physiological adaptation to the humid tropics with special reference to the West African Dwarf (WAD) goat. Trop Anim Health Prod 41:1005–1016CrossRefGoogle Scholar
  15. El-Masry KA (1987) The role of thyroxine in improving productivity of heat-stressed animals with different techniques. Ph.D. Thesis, Faculty of Agriculture, Zagazig University, ZagazigGoogle Scholar
  16. El-Masry KA, Habeeb AA (1989) Thyroid function in lactating Friesian cows and water buffalos under winter and summer Egyptian conditions. Proceedings of 3rd Egyptian British Conference on Animal, Fish and poultry production, Vol 2, Alexandria, pp 613–620Google Scholar
  17. Gaughan JB, Mader TL, Holt SM, Sullivan ML, Hahn GL (2009) Assessing the heat tolerance of 17 beef cattle genotypes. Int J Biometeorol. doi:10.1007/s00484-009-0233-4
  18. Gudev D, Popova-Ralcheva S, Moneva1 P, Aleksiev P, Peeva TZ, Penchev P, Ilieva I (2007) Physiological indices in buffaloes exposed to sun. Arch Zootechnica (10):127–132Google Scholar
  19. Habeeb AA (1987) The role of insulin in improving productivity of heat-stressed farm animals with different techniques. PhD thesis. Faculty of Agriculture, Zagazig University, Zagazig, EgyptGoogle Scholar
  20. Jain NC (1986) Schalm’s veterinary haematology. Lea and Febiger, PhiladelphiaGoogle Scholar
  21. Kamiya M, Kamiya Y, Tanaka M, Shioya S (2006) Milk protein production and plasma 3-methylhistidine concentration in lactating Holstein cows exposed to high ambient temperatures. Asian Austr J Anim Sci 19(8):1159–1163Google Scholar
  22. Kannan G, Terrill TH, Kouakou B, Gazal OS, Gelaye S, Amoah EA, Samake S (2000) Transportation of goats: effects on physiological stress responses and live weight loss. J Anim Sci 78:1450–1457Google Scholar
  23. Korde JP, Singh G, Varshney VP, Shukla DC (2007) Effects of long-term heat exposure on adaptive mechanism of blood acid-base in buffalo calves. Asian-Australas J Anim Sci 20(5):742–747Google Scholar
  24. Mader TL, Gaughan JB, Johnson LJ, Hahn GL (2009) Tympanic temperature in confined beef cattle exposed to excessive heat load. Int J Biometeorol. doi:10.1007/s00484-009-0229-0
  25. Marai IFM, Abdel-Samee AM, El-Gafaary MN (1991) Criteria of response and adaptation to high temperature for reproductive and growth traits in rabbits. Options Mediterraneennes Series A 17:127–134Google Scholar
  26. Marai IFM, El-Darawany AA, Fadiel A, Abdel-Hafez MAM (2007) Physiological traits as affected by heat stress in sheep—a review. Small Rumin Res 71:1–12CrossRefGoogle Scholar
  27. Martin GB, Rogar J, Blache D (2004) Nutritional and environmental effects on reproduction in small ruminants. Reprod Fert Dev 16:491–501CrossRefGoogle Scholar
  28. Maurya VP, Naqvi SMK, Joshi A, Mittal JP (2007) Effect of high temperature stress on physiological responses of Malpura sheep. Indian J Anim Sci 77:1244–1247Google Scholar
  29. McManus C, Paludo GR, Louvandini H, Gugel R, Sasaki LCB, Paiva SR (2009) Heat tolerance in Brazilian sheep: physiological and blood parameters. Trop Anim Health Prod 41:95–101CrossRefGoogle Scholar
  30. Moberg GP (2000) Biological responses to stress. Implications for animal welfare. In: Moberg GP, Mench JP (eds) Biology of animal stress. CAB International, Wallingford, pp 1–21Google Scholar
  31. Mohebbi-Fani M, Nazifi S, Rowghani E, Bahrami S, Jamshid O (2009) Thyroid hormones and their correlations with serum glucose, beta hydroxybutyrate, nonesterified fatty acids, cholesterol, and lipoproteins of high-yielding dairy cows at different stages of lactation cycle. Comp Clin Pathol 18:211–216CrossRefGoogle Scholar
  32. More T, Sahni KL (1979) Some haematological changes during three months after birth of Chokla lambs under semi-arid conditions. Indian Vet J 56:646–649Google Scholar
  33. More T, Sahni KL (1980) Certain biochemical responses of sheep and goat exposed to thermal stress. Indian J Anim Sci 50:1012–1014Google Scholar
  34. Moss DW, Henderson AK (1994) Tietz textbook of clinical chemistry, 3rd edn. Saunders, PhiladelphiaGoogle Scholar
  35. Nardone A, Ronchi B, Lacetera N, Bernabucci U (2006) Climatic effects on productive traits in livestock. Vet Res Commun 30(1):75–81CrossRefGoogle Scholar
  36. Naqvi SMK, Hooda OK (1991) Influence of thermal, nutritional and exercise stress on some blood parameters of native and crossbred sheep. Indian J Anim Sci 61:660–662Google Scholar
  37. Naqvi SMK, Rai AK (1991) Influence of dietary energy level on sheep for mutton during winter: effect on cardio respiratory responses, rectal temperature, some blood metabolites, enzymes and thyroidal hormones. Indian J Anim Sci 61:1126–1131Google Scholar
  38. Naqvi SMK, Joshi A, Das GK, Mittal JP (2001) Development and application of ovine reproductive technologies: an Indian experience. Small Rumin Res 39:199–208CrossRefGoogle Scholar
  39. Naqvi SMK, Sejian V (2010) Physiological adaptation of sheep to hot environmental conditions with special reference to climate change. In: Karim SA, Joshi A, Sankhyan SK, Shinde AK, Shakyawar DB, Naqvi SMK, Tripathi BN (eds) Climate change and stress management: sheep and goat production, 1st edn. Satish, Delhi, pp 259–282Google Scholar
  40. Nazifi S, Saeb M, Rowghani E, Kaveh K (2003) The influence of thermal stress on serum biochemical parameters of Iranian fat-tailed sheep and their correlation with triiodothyronine (T3), thyroxine (T4) and cortisol concentrations. Comp Clin Pathol 12:135–139CrossRefGoogle Scholar
  41. Nienaber JA, Hahn GL, Eigenberg RA (1999) Quantifying livestock responses for heat stress management: a review. Int J Biometeorol 42:183–188CrossRefGoogle Scholar
  42. Pereira AMF, Baccari F Jr, Titto EAL, Almeida JAA (2008) Effect of thermal stress on physiological parameters, feed intake and plasma thyroid hormones concentration in Alentejana, Mertolenga, Frisian and Limousine cattle breeds. Int J Biometeorol 52:199–208CrossRefGoogle Scholar
  43. Rae MT, Rhind SM, Miller DW, Brooks AN (2002) Maternal undernutrition alters triiodothyronine concentrations and pituitary response to GnRH in fetal sheep. J Endocrinol 173:449–455CrossRefGoogle Scholar
  44. Rai AK, More T, Singh M (1983) Certain blood attributes in different genetic groups of sheep on feed lot ration. Indian Vet J 60:839–841Google Scholar
  45. Rasooli A, Nouri M, Khadjeh GH, Rasekh A (2004) The influence of seasonal variations on thyroid activity and some biochemical parameters of cattle. Iranian J Vet Res 5(2):1383–1391Google Scholar
  46. Riis PM, Madsen A (1985) Thyroxine concentration and secretion rates in relation to pregnancy, lactation and energy balance in goats. J Endocrinol 107:421–427CrossRefGoogle Scholar
  47. Saber APR, Jalali MT, Mohjeri D, Akhoole AA, Teymuourluei HZN, Nouri M, Garachorlo S (2009) The effect of ambient temperature on thyroid hormones concentration and histopthological changes of thyroid gland in cattle in Tabriz, Iran. Asian J Anim Vet Adv 4(1):28–33CrossRefGoogle Scholar
  48. Sano H, Takahashi K, Fujita M, Ambo K, Tsuda T (1979) Effect of environmental heat exposure on physiological responses, blood constituents and parameters of blood glucose metabolism in sheep. Tohoku J Agr Res 30(2):76–86Google Scholar
  49. Sejian V, Srivastava RS, Varshney VP (2008a) Pineal-adrenal relationship: modulating effects of glucocorticoids on pineal function to ameliorate thermal-stress in goats. Asian Austr J Anim Sci 21:988–994Google Scholar
  50. Sejian V, Sanyal S, Das PK, Ghosh PR, Sivakumar B, Pandiyan GDV (2008b) Effect of unilateral adrenalectomy on the blood biochemistry of Black Bengal goats (Capra hircus). Turk J Vet Anim Sci 32(4):249–254Google Scholar
  51. Sejian V, Srivastava RS (2010) Effects of melatonin on adrenal cortical functions of Indian goats under thermal stress. Vet Med Int 2010:Article ID 348919, 6 pages, doi:10.4061/2010/348919 Google Scholar
  52. Sejian V, Srivastava RS, Varshney VP (2010a) Effect of short-term thermal stress on biochemical profile in Marwari goats. Indian Vet J 87(5):503Google Scholar
  53. Sejian V, Srivastava RS, Varshney VP (2010b) Effect of thermal stress on endocrine profile and phagocytosis index in Marwari goats. Indian Vet J (in press)Google Scholar
  54. Singh M, Rai AK, Karim SA (1983) Relative heat tolerance of Indian Nali and Soviet Merino x Nali halfbred sheep to continuous 35°C and 40°C temperature conditions. J Anim Physiol Anim Nutr 49:18–23Google Scholar
  55. Snedecor GW, Cochran WG (1994) Statistical methods, 8th edn. Iowa State University Press, AmesGoogle Scholar
  56. Spicer LJ, Crowe MA, Prendiville DJ, Goulding D, Enright WJ (1992) Systemic but not intraovarian concentrations of insulin-like growth factor-I are affected by short-term fasting. Biol Reprod 46:920–925CrossRefGoogle Scholar
  57. Tietz NW (1976) Clinical guide to laboratory tests. Saunders, PhiladelphiaGoogle Scholar
  58. Tietz NW (1995) Clinical Guide to laboratory tests, 3rd edn. Saunders, PhiladelphiaGoogle Scholar
  59. Todini I (2007) Thyroid hormones in small ruminants: effects of endogenous, environmental and nutritional factors. Animal 1:997–1008CrossRefGoogle Scholar
  60. Varley H (1980) Practical clinical biochemistry, vol 1, 5th edn. Heinemann, LondonGoogle Scholar
  61. Wingfield AS, Kitaysky JC (2002) Endocrine responses to unpredictable environmental events: stress or anti-stress hormone. Integr Comp Biol 42:600–609CrossRefGoogle Scholar
  62. Yousef HM (1985) Studies on some environmental factors affecting production and reproduction in some farm animals. MSc thesis. Faculty of Agriculture, Zagazig University, ZagazigGoogle Scholar

Copyright information

© ISB 2010

Authors and Affiliations

  • Veerasamy Sejian
    • 1
  • Vijai P. Maurya
    • 1
  • Sayeed M. K. Naqvi
    • 1
  1. 1.Adaptation Physiology Laboratory, Division of Physiology & BiochemistryCentral Sheep and Wool Research InstituteTonk DistrictIndia

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