Trees

, Volume 14, Issue 6, pp 334–338

Cloning of a Picea abies monosaccharide transporter gene and expression – analysis in plant tissues and ectomycorrhizas

  • U. Nehls
  • Joachim Wiese
  • Rüdiger Hampp
Original Article

Abstract 

Ectomycorrhizas are formed between certain soil fungi and fine roots of woody plants. An important feature of this symbiosis is the supply of photoassimilates to the fungus. Hexoses, formed from sucrose in the common apoplast at the root/fungus interface, can be taken up by both plant and fungal monosaccharide transporters. Recently we characterised a monosaccharide transporter from the ectomycorrhizal fungus Amanita muscaria. This transporter was up-regulated in mycorrhizas, thus increasing the hexose uptake capacity of the fungal partner in symbiosis. In order to characterise host (Picea abies) root monosaccharide transporters, degenerate oligonucleotide primers, designed to match conserved regions from known plant hexose transporters, were used to isolate a cDNA fragment of a transporter by PCR. This fragment was used to identify a presumably full length clone (PaMST1) in a P. abies/A. muscaria mycorrhizal cDNA library. The entire cDNA code for an open reading frame of 513 amino acids, revealing best homology to H+/monosaccharide transporters from Ara- bidopsis, Saccharum and Ricinus. PaMST1 was highly expressed in the hypocotyl and in roots of P. abies seedlings, but not in needles. Mycorrhiza formation led to a slight reduction of PaMST1 expression. The results are discussed with special reference to carbon allocation in ectomycorrhizas.

Key words  Picea abies Monosaccharide transporter Gene expression Ectomycorrhizas Carbon allocation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • U. Nehls
    • 1
  • Joachim Wiese
    • 1
  • Rüdiger Hampp
    • 1
  1. 1.Universität Tübingen, Botanisches Institut, Physiologische Ökologie der Pflanzen, Auf der Morgenstelle 1, 72076 Tübingen, Germany e-mail: uwe.nehls@uni-tuebingen.de Tel.: +49-7071-2977657, Fax: +49-7071-296155DE

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