pp 1–12 | Cite as

Comparative transcriptome analysis reveals positive effects of arbuscular mycorrhizal fungi inoculation on photosynthesis and high-pH tolerance in blueberry seedlings

  • Lu Yang
  • Qing-Qing Li
  • Yan Yang
  • Qiang Chen
  • Xuan Gao
  • Jia-Xin XiaoEmail author
Original Article
Part of the following topical collections:
  1. Mycorrhiza

Key message

Our results based on transcriptome data and physiological alterations give an account for enhancing high-pH tolerance in blueberry seedlings with AMF inoculation.


To understand the responses occurring in leaves of arbuscular mycorrhizal fungi (AMF)-inoculated plants under high-pH stress, we combined physiological analyses with leaf transcriptome profiles of AMF-inoculated and non-inoculated blueberry (Vaccinium corymbosum) cultivar “O’Neal” under optimal-pH (pH 4.2) and high-pH (pH 6.2) conditions. Comparative transcriptome analysis revealed 250 differentially expressed genes (DEGs) in AMF-inoculated plants when compared with non-inoculated plants under high-pH stress. These DEGs were involved in 37 metabolic pathways, such as photosynthesis, hormone metabolism, carbohydrate metabolism, amino acid metabolism, stress response, signal transduction, and antioxidation. Physiological analyses revealed that AMF-inoculated plants presented lower respiration and higher photosynthesis efficiency under high-pH stress, along with accumulation of photosystem II reaction center PsbP family protein, enhancement of amino acids content, and stronger secondary metabolites biosynthesis ability, when compared with non-inoculated plants. These results provide new insights into a probable mechanism of protection of photosynthesis and enhancement of high-pH tolerance in blueberry seedlings with AMF inoculation.


Mycorrhiza Vaccinium corymbosum Blueberry High-pH stress Transcriptome Photosynthesis 



This work was supported by the Provincial Natural Science Research Program of Higher Education of Anhui province (KJ2016SD24 and KJ2019A0484) and the Doctoral Program Foundation (2018) of Anhui Normal University (751863).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Lu Yang
    • 1
  • Qing-Qing Li
    • 1
  • Yan Yang
    • 1
  • Qiang Chen
    • 1
  • Xuan Gao
    • 1
  • Jia-Xin Xiao
    • 1
    Email author
  1. 1.Key Laboratory for the Conservation and Utilization of Important Biological Resources, Anhui Province, College of Life SciencesAnhui Normal UniversityWuhuChina

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