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Selfing of a single monoecious Populus tremula tree produces viable males, females and “supermales”

  • Matthias FladungEmail author
  • Marek Schildbach
  • Hans Hoenicka
  • Birgit Kersten
  • Niels A. Müller
Original Article
  • 41 Downloads

Abstract

Key message

Even though “supermale” (YY) poplar individuals are rarely found in natural populations or breeding collections, they can arise and can be as viable as regular male and female plants.

Abstract

Nearly all species of the genus Populus are dioecious, i.e., form male and female flowers on separate individuals. However, bisexual poplar can occasionally be found under natural conditions or during the breeding process. According to the XX/XY model of sex determination, “supermale” (YY) individuals should only occur in the selfed progeny of a genetically male (XY) tree and not in the one of a genetically female (XX) parent. We performed detailed molecular genetic analyses of a bisexual Populus tremula XY-individual (ZP8) and its selfed progeny. The ZP8 tree was confirmed as P. tremula by chloroplast and nuclear markers, and the progeny was genotyped by microsatellite markers to verify self-pollination. In the selfed progeny but also in about 420 different poplar individuals from several breeding programmes and the wild, the X and Y chromosomes were tracked using previously reported and newly developed markers. From two self-pollination experiments of the bisexual ZP8 tree, 39 S1-individuals were obtained. Application of X- and Y-specific molecular markers revealed 15 XY, 17 YY, and 7 XX individuals. Additional investigations of about 300 different poplar clones employed in several breeding programmes as well as about 120 individuals from wild populations revealed only three trees with two Y chromosomes. Indications for inbreeding depression in the selfed progeny were obtained during germination and glasshouse cultivation, but are independent from the sex chromosome combination. Even though YY-poplar individuals are rarely found in natural populations or breeding collections, they can arise and can be as viable as regular XY- and XX-plants.

Keywords

Bisexual Dioecy Inbreeding Poplar Sex chromosome TOZ19 

Notes

Acknowledgements

We thank K. Groppe, D. Ebbinghaus, M. Will, and O. Polak (all Thuenen-Institute of Forest Genetics, Grosshansdorf, Germany) for excellent technical assistance in the lab, and the Thuenen-Institute greenhouse staff (W. Graf, M. Hunger, G. Wiemann, R. Ebbinghaus, M. Spauszus) for plant cultivation. Thanks are due to Dr. B. Heinze (Federal Research Centre for Forests, Department of Forest Genetics, Vienna, Austria), Prof. A. Tsarev (All-Russian Research Institute of Forest Genetics, Voronezh, Russia), V. Schneck (Thuenen-Institute of Forest Genetics, Waldsieversdorf, Germany), M. Tubes (Bayerisches Amt für forstliche Saat- und Pflanzenzucht (ASP), Teisendorf, Germany), Dr. M. Meyer (TU Dresden, Dresden, Germany), Dr. H. Wolf (Staatsbetrieb Sachsenforst, Graupa, Germany), Dr. H. Liesebach, Dr. H. Schröder, Dr. M. Liesebach, and Dr. G. von Wühlisch (all Thuenen-Institute of Forest Genetics, Grosshansdorf, Germany), for providing plant material or DNA samples from P. tremula individuals. We also thank Prof. Dr. O. Nilsson (Umeå Plant Science Centre, Umeå, Sweden) for kindly providing the pHSP::AtFT gene construct. Funding was provided by a core grant of the Thünen Institute.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Supplementary material 1 (DOCX 44 KB)
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Supplementary material 2 (DOCX 21 KB)
468_2019_1817_MOESM3_ESM.xlsx (26 kb)
Supplementary material 3 (XLSX 25 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Thünen-Institute of Forest Genetics (TI-FG)GrosshansdorfGermany
  2. 2.Staatsbetrieb Sachsenforst (SBS)PirnaGermany

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