, Volume 33, Issue 1, pp 213–225 | Cite as

Molecular characterization and expression profiles of GRAS genes in response to abiotic stress and hormone treatment in Tamarix hispida

  • Peilong Wang
  • Liuqiang WangEmail author
  • Zhongyuan Liu
  • Tengqian Zhang
  • Yuanyuan Wang
  • Yabo Li
  • Caiqiu GaoEmail author
Original Article
Part of the following topical collections:
  1. Ecological Genetics


GRAS (GAI, RGA and SCR) domain proteins are plant-specific transcriptional regulators that play roles in developmental processes and stress responses. However, the function and molecular regulatory networks of woody plant GRAS under various physiological conditions are still unclear. As a step toward understanding the GRAS function in plants under diverse environmental stimuli, a total of 12 GRAS genes from Tamarix hispida were identified and characterized. Sequence analysis showed that most of these ThGRASs contained a complete GRAS DNA-binding domain and a variable N-terminal region. Phylogenetic analysis revealed that these GRAS members were divided into PAT1, SHR, LISCL, DLT and DELLA groups. The expressions of each individual GRAS under various abiotic stress conditions or hormone treatments were investigated by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR). qRT-PCR results showed that these ThGRASs were all differentially expressed in the roots or leaves of T. hispida under abiotic stress and hormone treatment, especially ThGRAS3, ThGRAS5, ThGRAS6, ThGRAS7 and ThGRAS10 genes, indicating that these genes were involved in abiotic stress responses and signaling pathways. This study provides a basis for the elucidation of the function of GRAS genes for future works.


GRAS Tamarix hispida Abiotic stress Expression pattern 



This work was supported by the Province in Heilongjiang Outstanding Youth Science Fund (JC2017004) and the National Natural Science Foundation of China (no. 31370676).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Data archiving statement

All the identified GRAS genes were deposited with GenBank with the GenBank accession numbers: ThGRAS1 (MG817391); ThGRAS2 (MG817392); ThGRAS3 (MG817393); ThGRAS4 (MG817394); ThGRAS5 (MG817395); ThGRAS6 (MG817396); ThGRAS7 (MG817397); ThGRAS8 (MG817398); ThGRAS9 (MG817399); ThGRAS10 (MG817400); ThGRAS11 (MG817401); ThGRAS12 (MG817402).

Supplementary material

468_2018_1771_MOESM1_ESM.doc (46 kb)
Supplementary material 1 (DOC 46 KB)
468_2018_1771_MOESM2_ESM.doc (29 kb)
Supplementary material 2 (DOC 29 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Peilong Wang
    • 1
  • Liuqiang Wang
    • 2
    Email author
  • Zhongyuan Liu
    • 1
  • Tengqian Zhang
    • 1
  • Yuanyuan Wang
    • 1
  • Yabo Li
    • 1
  • Caiqiu Gao
    • 1
    Email author
  1. 1.State Key Laboratory of Tree Genetics and BreedingNortheast Forestry UniversityHarbinChina
  2. 2.State Key Laboratory of Tree Genetics and Breeding, Research Institute of ForestryChinese Academy of ForestryBeijingChina

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