Can explant choice help resolve recalcitrance problems in in vitro propagation, a problem still acute especially for adult conifers?
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There are tissues distributed throughout the plant that have a higher morphogenic capacity than others in the plant, and these could perhaps solve recalcitrance problems.
For many conifer species, regeneration by organogenesis or somatic embryogenesis (SE) is still difficult and is often restricted to explants taken from juvenile donors. This review is based on the premise that there are tissues in the plant that are not normally used as explant, mostly because excising them in a viable state is difficult. Nevertheless, in cases where recalcitrance is a major problem, it may be worthwhile to pay closer attention to these tissues. Recalcitrance is a general problem, and discussion of it requires examples from the general literature. However, to restrict the scope of this review, preference will be given to conifer examples whenever possible.
KeywordsAsymmetric division In vitro Isolation Organogenesis Somatic embryogenesis Symplasm
Lateral root meristem
Capable of forming one or more organs
Shoot apical meristem
Capable of forming an embryo from a somatic cell that is similar to a zygotic embryo
I wish to thank Dr. Krystyna Klimaszewska and Dr. Patrick von Aderkas for their review of the manuscript.
Compliance with ethical standards
Conflict of interest
The author declares that he has no conflict of interest.
- Atta R, Laurens L, Boucheron-Dubuisson E, Guivarc’h A, Carnero E, Giraudat-Pautot V, Rech P, Chriqui D (2009) Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro. Plant J 57:626–644. doi: 10.1111/j.1365-313X.2008.03715.x CrossRefPubMedGoogle Scholar
- Bonga JM (2012) Recalcitrance in the in vitro propagation of trees. In: Proceedings of the IUFRO Working Party 2.09.02 conference: Integrating vegetative propagation, biotechnologies and genetic improvement for tree production and sustainable forest management, 25–28 June 2012, Brno, pp 37–46. http://www.iufro20902.org/Publications
- Bonga JM (2016) Conifer clonal propagation in tree improvement programs. In: Park YS, Bonga JM, Moon H-K (eds) Vegetative propagation of forest trees. National Institute of Forest Science (NiFoS), Seoul, pp 3–31. http://www.iufro20902.org/Publications
- Bonga JM, McInnis AH (1983) Origin and early development of roots in plantlets derived from embryo sections of Larix decidua in vitro. Can For Service Res Notes 3:12–14Google Scholar
- Cutter EG (1972) Plant anatomy: experiment and interpretation. Part 2 Organs. William Clowes & Sons Ltd, LondonGoogle Scholar
- Esau K (1965) Plant anatomy, 2nd edn. Wiley, New YorkGoogle Scholar
- Hecht V, Vielle-Calzada Hartog MV, Schmidt EDL, Boutilier K, Grossniklaus U, de Vries S (2001) The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in the developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol 127:803–816CrossRefPubMedPubMedCentralGoogle Scholar
- Klimaszewska K, Rutledge RG (2016) Is there potential for propagation of adult spruce trees through somatic embryogenesis? In: Park YS, Bonga JM, Moon H-K (eds) Vegetative propagation of forest trees. National Institute of Forest Science (NiFoS), Seoul, pp 195–210. http://www.iufro20902.org/Publications
- Monteuuis O, Lardet L, Montoro P, Berthouly M, Verdeil J-L (2011) Somatic embryogenesis and phase change in trees. In: Proceedings of the IUFRO Working Party 2.09.02 “Somatic embryogenesis of trees” conference on “Advances in somatic embryogenesis of trees and its application for the future forests and plantations”, 19–21 August, Suwon, pp 21–28Google Scholar
- Parizot B, Laplaze L, Ricaud L, Boucheron-Dubuisson E, Bayle V, Bonke M, De Smet I, Poethig SR, Helariutta Y, Haseloff J, Chirqui D, Beeckman T, Nussaume L (2008) Diarch symmetry of the vascular bundle in Arabidopsis root encompasses the pericycle and is reflected in distich lateral root initiation. Plant Physiol 146:140–148CrossRefPubMedPubMedCentralGoogle Scholar
- Steeves TA, Sussex IM (1972) Patterns in plant development. Prentice Hall, New Jersey, p 302Google Scholar
- Steward FC (1968) Growth and organization in plants. Addison-Wesley Publishing Company, Reading, p 564Google Scholar
- Tran Thanh Van K, Yilmaz-Lentz D, Trinh TH (1987) In vitro control of morphogenesis in conifers. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol 2., Specific principles and methods: growth and developmentsMartinus Nijhoff Publishers, Dordrecht, pp 168–182CrossRefGoogle Scholar
- Trontin J-F, Aronen T, Hargreaves C, Montalbán IA, Moncaleán P, Reeves C, Quoniou S, Lelu-Walter M-A, Klimaszewska K (2016) International effort to induce somatic embryogenesis in adult pines. In: Park YS, Bonga JM, Moon H-K (eds) Vegetative propagation of forest trees. National Institute of Forest Science (NIFoS), Seoul, pp 211–260. http://www.iufro20902.org/Publications