Can explant choice help resolve recalcitrance problems in in vitro propagation, a problem still acute especially for adult conifers?
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There are tissues distributed throughout the plant that have a higher morphogenic capacity than others in the plant, and these could perhaps solve recalcitrance problems.
For many conifer species, regeneration by organogenesis or somatic embryogenesis (SE) is still difficult and is often restricted to explants taken from juvenile donors. This review is based on the premise that there are tissues in the plant that are not normally used as explant, mostly because excising them in a viable state is difficult. Nevertheless, in cases where recalcitrance is a major problem, it may be worthwhile to pay closer attention to these tissues. Recalcitrance is a general problem, and discussion of it requires examples from the general literature. However, to restrict the scope of this review, preference will be given to conifer examples whenever possible.
KeywordsAsymmetric division In vitro Isolation Organogenesis Somatic embryogenesis Symplasm
Lateral root meristem
Capable of forming one or more organs
Shoot apical meristem
Capable of forming an embryo from a somatic cell that is similar to a zygotic embryo
I wish to thank Dr. Krystyna Klimaszewska and Dr. Patrick von Aderkas for their review of the manuscript.
Compliance with ethical standards
Conflict of interest
The author declares that he has no conflict of interest.
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