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Trees

, Volume 30, Issue 4, pp 1389–1402 | Cite as

Genome-wide transcriptomic profiles reveal multiple regulatory responses of poplar to Lonsdalea quercina infection

  • Jia Hou
  • Qiuming Wu
  • Tao Zuo
  • Limin Guo
  • Jupu Chang
  • Jinhuan Chen
  • Yanwei WangEmail author
  • Wei HeEmail author
Original Article
  • 323 Downloads
Part of the following topical collections:
  1. Phytopathology

Abstract

Key message

Remarkable gene expression changes and responsive DEGs were found in poplar upon pathogen attack and provide important clues to the molecular mechanism of defense responses in plants and gene functional studies of trees.

Abstract

The disease Lonsdalea canker affects the growth and productivity of poplar (Populus spp.), often resulting in death of poplar trees. To obtain expression profiles of differentially expressed genes (DEGs) for the response of poplar to pathogens, transcriptomic sequencing was performed using bark from 2-year-old P. × euramericana Guinier trees inoculated (IN) or mock-inoculated (KK) with Lonsdalea quercina. In total, 7,558,430 and 7,149,345 clean reads were generated in the KK and IN libraries, respectively. Gene expression profiles identified a total of 7098 DEGs, among which there were nearly twice as many down-regulated (4635) as up-regulated (2463) genes post-inoculation. Expression was further validated and dynamic responses analyzed by qRT-PCR. These DEGs were predominantly related to the biosynthesis of secondary metabolites, plant hormone signal transduction, regulation of autophagy, and ABC transport upon L. quercina infection. In addition, DEGs involved in the flavonoid biosynthesis pathway, and JA and SA-mediated signal transduction pathways might play important roles in the L. quercina infection response in P. × euramericana. The pathogen-responsive DEGs identified in this investigation provide important clues to the molecular mechanism of defense responses in plants and gene functional studies of trees.

Keywords

Poplar Lonsdalea canker RNA-seq DEGs 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31470668, 31200511), the China National Forestry Department Public Benefit Research Foundation (201104054), and Program for Changjiang Scholars and Innovative Research Team in University (No. IRT13047).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Data archiving statement

RNA-Seq data of poplars infected or mock-infected with L. quercina can be available at the NIH Short Read Archive database (http://www.ncbi.nlm.nih.gov/sra) under the accession number SRP057770.

Supplementary material

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.National Engineering Laboratory for Tree Breeding, Key Laboratory for Silviculture and Conservation of Ministry of Education, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, College of Biological Sciences and Biotechnology, College of ForestryBeijing Forestry UniversityBeijingChina
  2. 2.Forestry Research Institute of PuyangPuyangChina

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