Endo-1,4-β-glucanase gene involved into the rapid elongation of Phyllostachys heterocycla var. pubescens
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Endo - 1,4 - β - glucanase gene was isolated from Phyllostachys heterocycla var. pubescens internodes. It is engaged in the culm or stem development by regulation of the biosynthesis of cellulose.
The cellulase protein endo-1,4-β-glucanase is a member in the large glycosyl hydrolase gene family 9 (GH 9). It is widely distributed in plants, animals, microorganisms and plays roles in cell wall metabolism, including cellulose biosynthesis and degradation, modification of cell wall polysaccharides and cell wall loosening during cell elongation. Our previous studies have identified a gene homologous to endo-1,4-β-glucanase and found its expression pattern changed significantly during the rapid elongation of Phyllostachys heterocycla var. pubescens internodes. In this work, we isolated the full length endo-1,4-β-glucanase gene from bamboo shoot and named as Phbeta-1,4-glu. Further characterization showed that Phbeta-1,4-glu belongs to GH 9 family, a conserved family in bamboo with slight variation in intron numbers and positions. Phylogenetic analysis revealed that P. heterocycle GH 9 genes exhibit a diverse phylogeny with rice, maize, Brachypodium, poplar and Arabidopsis. Among them, bamboo GH 9 genes show a closer relationship to ones of Brachypodium. RT-PCR analysis demonstrated that the expression pattern of Phbeta-1,4-glu varied among different tissues of bamboo shoot with a lowest expression level in the tender parts. The function of Phbeta-1,4-glu in bamboo’s height growth and cellulose content was confirmed via its transformation into the model plant-Arabidopsis. Functions of Phbeta-1,4-glu in bamboo shoot have been analyzed through a combinational methods of gene structure and phylogeny. Its gene expression pattern has been investigated and its function verified. The results confirmed that Phbeta-1,4-glu engages in the rapid elongation of bamboo culm from cellulose biosynthesis to cellulose degradation. Current investigations provide valuable information for its functional studies and potential utilization in future.
Keywordsendo-1,4-β-glucanase Phyllostachys heterocycla var. pubescens Internodes Phylogenetic relationship Cellulose
This work was supported by the grant from the Program of Natural Science Foundation of Zhejiang Province (LR12C16001), and the National Natural Science Foundation of China (31270645, 31500542 and 31470615), and the Opening Foundation of the Key Forestry Disciplines of Zhejiang Province (KF201301).
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Conflict of interest
The authors declare that they have no conflict of interest.
- Buchanan M (2011) Cellulose, stem strength and the Endo-(1, 4)-β-Glucanase gene family in Barley and Maize. Ph. D. thesis, The University of Adelaide, Adelaide, pp 1–405Google Scholar
- Chen B (2010) Application of intron in bioinformatics researches and transgenic engineering. Chem Life 30:59–63Google Scholar
- Chen YJ, Sun CW (2010) Transgenic study of chloroplast translocon gene regulation in Arabidopsis thaliana. Bot Stud 51:147–153Google Scholar
- Deng G, Zeng G, Wang L (1988) Studies on cell division of intercalary meristem and intercalary growth of Phyllostachys pubescens and Phyllostachys bambusoides, Phyllostachys heteroclada Oliv. Acta Sci Nat Univ Norm Hunan 11:244–250Google Scholar
- Dong LN (2007) Studies on developmental anatomy of elongated growth about bamboo culms. Master thesis, Nanjing Forestry University, Nanjing, pp 1–69Google Scholar
- Du LZ, Jing AW (2010) cDNA cloning and expression of Phyllostachys praecox Z.d.Chu et C.S.Chao cellulose synthase gene. Acta Agric Univ 32:0535–0540Google Scholar
- Fagard M, Desnos T, Desprez T, Goubet F, Refregier G, Mouille G, McCann M, Rayon C, Vernhettes S, Höfte H (2000) PROCUSTE1 encodes a cellulose synthase required for normal cell elongation specifically in roots and dark-grown hypocotyls of Arabidopsis. Plant Cell 12:2409–2423CrossRefPubMedPubMedCentralGoogle Scholar
- Feng JY (2010) Studies on primary thickening growth mechanism of Phyllostachys edulis shoot bud. Master thesis, Nanjing Forestry University, Nanjing, pp 1–65Google Scholar
- Grass Phylogeny Working Group, Barker NP, Clark LG, Davis JI, Duvall MR, Guala GF, Hsiao C, Kellogg EA, Peter Linder H, Mason-Gamer RJ, Mathews SY, Simmons MP, Soreng RJ, Spangler RE (2001) Phylogeny and subfamilial classification of the grasses (Poaceae). Ann Missouri Bot Garden 88: 373–457Google Scholar
- He SE (2009) cDNA library construction and molecular cloning of cellulose synthase gene (PeCesA12 and PeCesA11) from Moso bamboo. Master thesis, Zhejiang Forestry University, Zhejiang, pp 1–46Google Scholar
- Jiang ZH (2002) World bamboo and rattan. LiaoNing Science and Technology Publishing House, LiaoNingGoogle Scholar
- Li ZL (1996) Plant tissue producer. Peking University Press, BeijingGoogle Scholar
- Li C, Qi L, Wang J, Wang Y, Shi S, Zhang S (2004) Cellulose synthase gene and cellulose biosynthesis in plants. Biotechnol Bull 2005:5–11Google Scholar
- Magel E, Kruse S, Lütje G, Liese W (2005) Soluble carbohydrates and acid invertases involved in the rapid growth of developing culms in Sasa palmata (Bean). Camus Bamboo Sci Cult 19:23–29Google Scholar
- Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang HB, Wang XY, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang LF, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Rahman M, Ware D, Westhoff P, Mayer KFX, Messing J, Rokhsar DS (2009) The Sorghum bicolor genome and the diversification of grasses. Nature 457:551–556CrossRefPubMedGoogle Scholar
- Peng ZH, Lu Y, Li LB, Zhao Q, Feng Q, Gao ZM, Lu HY, Hu T, Yao N, Liu KY, Li Y, Fan DL, Guo YL, Li WJ, Lu YQ, Weng QJ, Zhou CC, Zhang L, Huang T, Zhao Y, Zhu CR, Liu XG, Yang XW, Wang T, Miao K, Zhuang CY, Cao XL, Tang WL, Liu GS, Liu YL, Chen J, Liu ZJ, Yuan LC, Liu ZH, Huang XH, Lu TT, Fei BH, Ning ZM, Han B, Jiang ZH (2013a) The draft genome of the fast-growing non-timber forest species moso bamboo (Phyllostachys heterocycla). Nat Genet 45:456–461CrossRefPubMedGoogle Scholar
- Salse J, Bolot S, Throude M, Jouffe V, Piegu B, Quraishi UM, Calcagno T, Cooke R, Delseny M, Feuillet C (2008) Identification and characterization of shared duplications between rice and wheat provide new insight into grass genome evolution. Plant Cell 20:11–24CrossRefPubMedPubMedCentralGoogle Scholar
- Sami AJ, Shakoori A (2008) Biochemical characterization of endo-1, 4-β-D-glucanase activity of a green insect pest Aulacophora foveicollis (Lucas). Life Sci J 5(2):30–36Google Scholar
- Sato S, Kato T, Kakegawa K, Ishii T, Liu YG, Awano T, Takabe K, Nishiyama Y, Kuga S, Sato S, Nakamura Y, Tabata S, Shibata D (2001) Role of the putative membrane-bound endo-1, 4-β-glucanase KORRIGAN in cell elongation and cellulose synthesis in Arabidopsis thaliana. Plant Cell Physiol 42:251–263CrossRefPubMedGoogle Scholar
- Shani Z, Dekel M, Roiz L, Horowitz M, Kolosovski N, Lapidot S, Alkan S, Koltai H, Tsabary G, Goren R, Shoseyov O (2006) Expression of endo-1, 4-β-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening. Plant Cell Rep 25:1067–1074CrossRefPubMedGoogle Scholar
- Ueda K (1960) Studies on the physiology of Bamboo, with reference to practical application. Reference data No. 34Google Scholar
- Wu LS, Jin XC, Yang YC, Huang HS, Chen ST, Yao WF, Yao FP (2010) cDNA cloning and expression analysis of Phyllostachys edulis beta-1, 4-glycosidase gene. Biotechnol Bull 3:026Google Scholar
- Xiong W, Ding Z, Li Y (1980) Intercalary meristem and internodal elongation of bamboo plants. Scientia Silvae Sinicae 16:81–89Google Scholar
- Zhang YQ, Liang JH, Li BX (2007) Application of β-glycosidase to cellulose bio-degradation. J Tianjin Univ 3:15Google Scholar