, Volume 29, Issue 4, pp 1197–1205 | Cite as

Impact of the laurina mutation in Coffea arabica L. on semi-dwarfism, cell number and hormonal profiles in hypocotyls of seedlings growing under daylight

  • Sophie Adler
  • Jean-Luc Verdeil
  • Geneviève Conejero
  • Irina L. Zaharia
  • Amélie Sarrazin
  • Julien Hoareau
  • Isabelle Fock-Bastide
  • Michel NoirotEmail author
Original Paper


Key message

Hypocotyl semi-dwarfism in BP was only due to less cell number. Daylight was required to inhibit cell division in the mutant. ABA, IAA and cytokinins would be involved.


Coffea arabica ‘Laurina’ is a natural mutant of Coffea arabica ‘Bourbon’ (B) and is known under the trade name ‘Bourbon Pointu’ (BP). Under daylight, the laurina mutation leads to pleiotropic effects, including semi-dwarf hypocotyls. At the opposite, semi-dwarfism of BP seedlings disappeared under darkness conditions. The first step was to describe the morphological impact of the mutation in seedlings growing under daylight by comparison with seedlings growing under darkness. As the hypocotyl length was mainly affected, the second step was to investigate histological modifications in the organ comparing B and BP seedlings growing under daylight. Result of this investigation indicated that the mutation does not impact on cell length. Moreover, cytometry analyses showed absence of endoreduplication. Actually, the mutation influenced the cell number and this effect appeared before the 40th day after sowing. The length difference of hypocotyls between B and BP was due to lower cell number in BP, indicating possible involvement of phytohormones. Investigations showed the decrease of cytokinin and auxin levels in BP compared to B, while the cytokinin/auxin ratio remained constant in both varieties. By contrast, abscisic acid content increased in BP. Concurrently these results indicate the lowering of cell division, due to the mutation.


Coffea arabica ‘Laurina’ Hypocotyl semi-dwarfism Cell division Auxin Cytokinin Abscisic acid 


Author contribution statement

Sophie Adler: she carried out experiments leading to histological and hormonal approaches. She did cytometry experiments, histological measures, statistical analyses, and paper writing. Jean-Luc Verdeil: he coached histological approaches and participated in paper correction. Geneviève Conéjéro: she coached microscope use, and participated in paper correction. Irina L. Zaharia: she coached hormonal dosages in the laboratory and participated in paper correction and English improvement. Amélie Sarrazin: she coached cytometry experiment. Julien Hoareau: he was at the origin of the paper, emphasizing the role of daylight on seedling morphology in the mutant. Isabelle Fock-Bastide: she participated in the paper writing. Michel Noirot: he participated in statistical analyses and paper writing.


This study was financially supported by the European Union, the Conseil Régional of Réunion and the French Institut pour la Recherche et le Développement (IRD). The authors thank Dr. Xiumei Han, Vera Cekic and Dang Van for excellent technical assistance. Authors also thank Pr. Charrier for its helpful comments.

Conflict of interest

The authors declare that they have no conflict of interest

Supplementary material

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Supplementary material 1 (TXT 0 kb)
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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Sophie Adler
    • 1
  • Jean-Luc Verdeil
    • 2
  • Geneviève Conejero
    • 3
  • Irina L. Zaharia
    • 4
  • Amélie Sarrazin
    • 5
  • Julien Hoareau
    • 1
  • Isabelle Fock-Bastide
    • 1
  • Michel Noirot
    • 6
    Email author
  1. 1.Pôle de Protection des PlantesUniversité de la Réunion, UMR Cirad-Université PVBMTSt PierreRéunion, France
  2. 2.Laboratoire PHIV,UMR AGAP INRA/CIRAD/SupAgroMontpellierFrance
  3. 3.Laboratoire PHIV, UMR BPMP INRA/CNRS/SupAgro/UM IIMontpellierFrance
  4. 4.Aquatic and Crop Resource DevelopmentNational Research Council of CanadaSaskatoonCanada
  5. 5.RIO Imaging platformMontpellierFrance
  6. 6.Pôle de Protection des PlantesIRD, UMR Cirad-Université PVBMTSt PierreRéunion, France

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