, Volume 29, Issue 2, pp 515–526 | Cite as

Differences in intra-annual wood formation in Picea abies across the treeline ecotone, Giant Mountains, Czech Republic

  • Václav TremlEmail author
  • Jakub Kašpar
  • Hana Kuželová
  • Vladimír Gryc
Original Paper
Part of the following topical collections:
  1. Tree Rings


Key message

Picea abies requires warming of both the above- and belowground parts of the tree for full resumption of cambial activity.


Elevation-related decrease in growing season temperatures is a highly important factor in limiting tree growth in cold environments such as alpine treeline ecotones. In this study, we aimed to identify radial growth timing differences in Picea abies (L.) Karst. between the lower (timberline) and upper (treeline) parts of an alpine treeline ecotone. Over three growing seasons, soil and air temperatures were measured and phenology of wood formation was analyzed at two sites separated by 140 m of elevation in the Giant Mountains, Czech Republic. The results showed that there were two periods with significant differences in wood phenology between timberline and treeline. In the early part of the growing season, higher ambient temperatures at timberline led to higher number of cambial and enlarging cells here than at treeline. In the second part of the growing season, the bigger and/or more numerous tracheids at timberline than at treeline required more time for maturation. Significant delay in the beginning of wood formation at treeline in comparison to timberline was observed only in 2011, when soil was frozen markedly longer at treeline. We found that cambial activity significantly increased when soil temperature increased from near zero to a threshold temperature of 4–5 °C. We therefore suggest that for P. abies both the above- and belowground parts of the tree must be sufficiently warm for full resumption of cambial activity.


Cambium Xylogenesis Giant Mountains Norway spruce Tree ring Elevation gradient 


Author contribution statement

VT coordinated research, collected, analyzed data (2010) and wrote the manuscript; JK and HK collected and analyzed data (2011, 2012); VG discussed results and commented on the manuscript.


This study was funded by grant project GACR P504/11/P557. J. Kašpar and H. Kuželová received support by the project SVV 260078/2014 and V. Gryc was supported by the European Social Fund and the state budget of the Czech Republic, Project “Indicators of Trees Vitality Reg. No. CZ.1.07/2.3.00/20.0265”. We appreciate the KRNAP authority for technical support and for permission to conduct research in a protected area. We are grateful to T. Ponocná for laboratory assistance and to J. Rosenthal for improving the English. Furthermore, we thank two anonymous reviewers for their helpful comments.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Aloni R, Aloni E, Langhans M, Ullrich CI (2006) Role of auxin in regulating Arabidopsis flower development. Planta 223:315–328. doi: 10.1007/s00425-005-0088-9 CrossRefPubMedGoogle Scholar
  2. Alvarez-Uria P, Körner C (2007) Low temperature limits of root growth in deciduous and evergreen temperate tree species. Funct Ecol 21:211–218. doi: 10.1111/j.1365-2435.2007.01231.x CrossRefGoogle Scholar
  3. Anfodillo T, Deslauriers A, Menardi R, Tedoldi L, Petit G, Rossi S (2012) Widening of xylem conduits in a conifer tree depends on the longer time of cell expansion downwards along the stem. J Exp Bot 63:837–845. doi: 10.1093/jxb/err309 CrossRefPubMedCentralPubMedGoogle Scholar
  4. Barbosa SM, Scotto MG, Alonso AM (2011) Summarising changes in air temperature over Central Europe by quantile regression and clustering. Nat Hazards Earth Syst Sci 11:3227–3233. doi: 10.5194/nhess-11-3227-2011 CrossRefGoogle Scholar
  5. Begum S, Nakaba S, Yamagishi Y, Oribe Y, Funada R (2013) Regulation of cambial activity in relation to environmental conditions: understanding the role of temperature in wood formation of trees. Physiol Plant 147:46–54CrossRefPubMedGoogle Scholar
  6. Büntgen U, Frank DC, Schmidhalter M, Neuwirth B, Seifert M, Esper J (2006) Growth/climate response shift in a long subalpine spruce chronology. Trees 20:99–110. doi: 10.1007/s00468-005-0017-3 CrossRefGoogle Scholar
  7. Büntgen U, Frank DC, Kaczka RJ, Verstge A, Zwijacz-Kozica T, Esper J (2007) Growth responses to climate in a multi-species tree-ring network in the Western Carpathian Tatra Mountains, Poland and Slovakia. Tree Physiol 27:689–702CrossRefPubMedGoogle Scholar
  8. Ceppi P, Scherrer SC, Fischer AM, Appenzaller Ch (2012) Revisiting Swiss temperature trends 1959–2008. Int J Climatol 32:203–213. doi: 10.1002/joc.2260 CrossRefGoogle Scholar
  9. Chaffey N (2002) Introduction. In: Chaffey N (ed) Wood formation in trees (cell and molecular biology techniques). Tylor and Francis, London, pp 1–8CrossRefGoogle Scholar
  10. Cuny EH, Rathgeber CBK, Frank D, Fonti P, Fournier M (2014) Kinetics of tracheid development explain conifer tree-ring structure. New Phytol 203:1231–1241. doi: 10.1111/nph.12871 CrossRefPubMedGoogle Scholar
  11. Fonti P, Solomonoff N, García-González I (2007) Earlywood vessels of Castanea sativa record temperature before their formation. New Phytol 173:562–570. doi: 10.1111/j.1469-8137.2006.01945.x CrossRefPubMedGoogle Scholar
  12. Friml J (2003) Auxin transport—shaping the plant. Curr Opin Plant Biol 6:7–12. doi: 10.1016/S1369-5266(02)00003-1 CrossRefPubMedGoogle Scholar
  13. Głowicki B (1998) Long-term temperature record of Snezka station. In: Sarosiek J, Stursa J (eds) Geoekologiczne problemy Karkonoszy I. Acarus, Poznaň, pp 117–123 (in Polish with English abstract)Google Scholar
  14. Gorsuch DM, Oberbauer SF (2002) Effects of mid-season frost and elevated growing season temperature on stomatal conductance and specific xylem conductivity of the arctic shrub, Salix pulchra. Tree Physiol 22:1027–1034. doi: 10.1093/treephys/22.14.1027 CrossRefPubMedGoogle Scholar
  15. Gričar J, Zupančič M, Čufar K, Koch G, Schmitt U, Oven P (2006) Effect of local heating and cooling on cambial activity and cell differentiation in the stem of Norway spruce (Picea abies). Ann Bot 97:943–951. doi: 10.1093/aob/mcl050 CrossRefPubMedCentralPubMedGoogle Scholar
  16. Gričar J, Prislan P, Gryc V, Vavrčík H, de Luis M, Čufar K (2014) Plastic and locally adapted phenology in cambial seasonality and production of xylem and phloem cells in Picea abies from temperate environments. Tree Physiol 34:869–881. doi: 10.1093/treephys/tpu026 CrossRefPubMedGoogle Scholar
  17. Gruber A, Baumgartner D, Zimmermann J, Oberhuber W (2009) Temporal dynamic of wood formation in Pinus cembra along the alpine treeline ecotone and the effect of climate variables. Trees 23:623–635. doi: 10.1007/s00468-008-0307-7 CrossRefPubMedCentralPubMedGoogle Scholar
  18. Gryc V, Hacura J, Vavrčík H, Urban J, Gebauer R (2012) Monitoring of xylem formation in Picea abies under drought stress influence. Dendrobiology 67:15–24Google Scholar
  19. Hartl-Meier C, Dittmar C, Zang C, Rothe A (2014) Mountain forest growth response to climate change in the Northern Limestone Alps. Trees 28:819–829. doi: 10.1007/s00468-014-0994-1 CrossRefGoogle Scholar
  20. Hoch G, Körner Ch (2012) Global patterns of mobile carbon stores in trees at the high-elevation tree line. Global Ecol Biogeogr 21:861–871. doi: 10.1111/j.1466-8238.2011.00731.x CrossRefGoogle Scholar
  21. Holtmeier F-K (2009) Mountain timberlines: ecology, patchiness, and dynamics (Advances in Global Change Research), 2nd edn. Springer, BerlinCrossRefGoogle Scholar
  22. Kirdyanov A, Hughes M, Vaganov E, Schweingruber F, Silkin P (2003) The importance of early summer temperature and date of snow melt for tree growth in the Siberian Subarctic. Trees 17:61–69. doi: 10.1007/s00468-002-0209-z CrossRefGoogle Scholar
  23. Körner Ch (1998) A re-assessment of high elevation treeline positions and their explanation. Oecologia 115:445–459CrossRefGoogle Scholar
  24. Körner Ch (2012a) Alpine treelines: functional ecology of the global high elevation tree limits. Springer, BaselCrossRefGoogle Scholar
  25. Körner Ch (2012b) Treelines will be understood once the functional difference between a tree and a shrub is. Ambio 41:197–206. doi: 10.1007/s13280-012-0313-2 CrossRefPubMedCentralPubMedGoogle Scholar
  26. Körner Ch, Hoch G (2006) A test of treeline theory on a montane permafrost island. Arctic Antarct Alp Res 38:113–119CrossRefGoogle Scholar
  27. Körner Ch, Paulsen J (2004) A world-wide study of high altitude treeline temperatures. J Biogeogr 31:713–732CrossRefGoogle Scholar
  28. Larson PR (1969) Wood formation and the concept of wood quality. Yale Univeristy, New HavenGoogle Scholar
  29. Lenz A, Hoch G, Körner Ch (2013) Early season temperature controls cambial activity and total tree ring width at the alpine treeline. Plant Ecol Divers 6:365–375. doi: 10.1080/17550874.2012.711864 CrossRefGoogle Scholar
  30. Lupi C, Morin H, Deslauriers A, Rossi S (2010) Xylem phenology and wood production: resolving the chicken-or-egg dilemma. Plant Cell Environ 33:1721–1730. doi: 10.1111/j.1365-3040.2010.02176.x CrossRefPubMedGoogle Scholar
  31. Lupi C, Morin H, Deslauriers A, Rossi S (2011) Xylogenesis in black spruce: does soil temperature matter? Tree Physiol 32:74–82. doi: 10.1093/treephys/tpr132 CrossRefPubMedGoogle Scholar
  32. Mayr S, Wieser G, Bauer H (2006) Xylem temperatures during winter in conifers at the alpine timberline. Agric For Meteorol 137:81–88. doi: 10.1016/j.agrformet.2006.02.013 CrossRefGoogle Scholar
  33. Migala K (2005) Climatic belts in the European mountains and the issue of global changes. Stud Geograf 78:1–149 (in Polish with English summary)Google Scholar
  34. Mitchell TD, Jones RG (2005) An improved method of constructing a database of monthly climate observations and associated high-resolution grids. Int J Climatol 25:693–712. doi: 10.1002/joc.1181 CrossRefGoogle Scholar
  35. Moser L, Fonti P, Büntgen U, Esper J, Luterbacher J, Franzen J, Frank D (2009) Timing and duration of European larch growing season along altitudinal gradients in the Swiss Alps. Tree Physiol 30:225–233. doi: 10.1093/treephys/tpp108 CrossRefPubMedGoogle Scholar
  36. Oberhuber W (2004) Influence of climate on radial growth of Pinus cembra within the alpine timberline ecotone. Tree Physiol 24:294–301CrossRefGoogle Scholar
  37. Oribe Y, Kubo T (1997) Effect of heat on cambial reactivation during winter dormancy in evergreen and deciduous conifers. Tree Physiol 17:81–87CrossRefPubMedGoogle Scholar
  38. Petit G, Anfodillo T, Carraro V, Grani F, Carrer M (2011) Hydraulic constraints limit height growth in trees at high altitude. New Phytol 189:241–252. doi: 10.1111/j.1469-8137.2010.03455.x CrossRefPubMedGoogle Scholar
  39. Régent Instruments Inc. (2011) (Accessed 30 June 2014)
  40. Rossi S, Deslauriers A, Morin H (2003) Application of the Gompertz equation for the study of xylem cell development. Dendrochronologia 21:33–39. doi: 10.1078/1125-7865-00034 CrossRefGoogle Scholar
  41. Rossi S, Anfodillo T, Menardi R (2006) Trephor a new tool for sampling microcores from tree stems. IAWA J 27:89–97CrossRefGoogle Scholar
  42. Rossi S, Deslauriers A, Anfodillo T, Carraro V (2007) Evidence of threshold temperatures for xylogenesis in conifers at high altitudes. Oecologia 152:1–12CrossRefPubMedGoogle Scholar
  43. Rossi S, Deslauriers A, Gričar J et al (2008a) Critical temperatures for xylogenesis in conifers of cold climates. Global Ecol Biogeogr 17:696–707. doi: 10.1111/j.1466-8238.2008.00417.x CrossRefGoogle Scholar
  44. Rossi S, Deslauriers A, Anfodillo T, Carrer M (2008b) Age-dependent xylogenesis in timberline conifers. New Phytol 177:199–208. doi: 10.1111/j.1469-8137.2007.02235.x PubMedGoogle Scholar
  45. Rossi S, Anfodillo T, Čufar K, Cuny HE, Deslauriers A, Fonti P, Frank D, Gričar J, Gruber A, King GM, Krause C, Morin H, Oberhuber W, Prislan P, Rathgeber CBK (2013) A meta-analysis of cambium phenology and growth: linear and non-linear patterns in conifers of the northern hemisphere. Ann Bot 112:1911–1920. doi: 10.1093/aob/mct243 CrossRefPubMedCentralPubMedGoogle Scholar
  46. Savidge RA (2000) Intrinsic regulation of cambial growth. J Plant Growth Regul 20:52–77. doi: 10.1007/s003440010002 CrossRefGoogle Scholar
  47. Savva Y, Oleksyn J, Reich PB, Tjoelker MG, Vaganov EA, Modrzynski J (2006) Interannual growth response of Norway spruce to climate along an altitudinal gradient in the Tatra Mountains, Poland. Trees 20:735–746. doi: 10.1007/s00468-006-0088-9 CrossRefGoogle Scholar
  48. Seo J-W, Eckstein D, Jalkanen R, Rickebusch S, Schmitt U (2008) Estimating the onset of cambial activity in Scots pine in northern Finland by means of the heat-sum approach. Tree Physiol 28:105–112CrossRefPubMedGoogle Scholar
  49. Simard S, Giovannelli A, Treydte K, Traversi ML, King ML, Frank GM, Fonti P (2013) Intra-annual dynamics of non-structural carbohydrates in the cambium of mature conifer trees reflects radial growth demands. Tree Physiol 33:913–923. doi: 10.1093/treephys/tpt075 CrossRefPubMedGoogle Scholar
  50. Štursa J, Jeník J, Kubíková J, Rejmánek M, Sýkora T (1973) Snow cover in the West Giant Mountains during extreme winter 1969/1970 and its ecological significance. Opera Corcontica 10:111–146 (In Czech with English abstract)Google Scholar
  51. Tolasz R, Míková T, Valeriánová A, Voženílek V (2007) Climate atlas of Czechia, 1st edn. Czech Hydrometeorological Institute, PragueGoogle Scholar
  52. Treml V, Ponocná T, Büntgen U (2012) Growth trends and temperature responses of treeline Norway spruce in the Czech-Polish Sudetes Mountains. Clim Res 55:91–103. doi: 10.3354/cr01122 CrossRefGoogle Scholar
  53. Turcotte A, Morin H, Krause C, Deslauriers A, Thibeault-Martel M (2009) The timing of spring rehydration and its relation with the onset of wood formation in black spruce. Agric For Meteorol 149:1403–1409. doi: 10.1016/j.agrformet.2009.03.010 CrossRefGoogle Scholar
  54. Ursache R, Nieminen K, Helariutt Y (2013) Genetic and hormonal regulation of cambial development. Physiol Plant 147:36–45CrossRefPubMedGoogle Scholar
  55. Vittoz P, Rulence B, Freléchoux F (2008) Effects of climate and land-use change on the establishment and growth of cembrain pine (Pinus cembra L.) over the altitudinal treeline ecotone in the Central Swiss Alps. Arctic Antarct Alp Res 40:225–232. doi: 10.1657/1523-0430 CrossRefGoogle Scholar
  56. Wiley E, Helliker B (2012) A re-evaluation of carbon storage in trees lends greater support for carbon limitation to growth. New Phytol 195:285–289. doi: 10.1111/j.1469-8137.2012.04180.x CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Václav Treml
    • 1
    Email author
  • Jakub Kašpar
    • 1
  • Hana Kuželová
    • 1
  • Vladimír Gryc
    • 2
  1. 1.Department of Physical Geography and Geoecology, Faculty of ScienceCharles University in PraguePragueCzech Republic
  2. 2.Department of Wood Science, Faculty of Forestry and Wood TechnologyMendel University in BrnoBrnoCzech Republic

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