Development of a genetic linkage map for Pinus radiata and detection of pitch canker disease resistance associated QTLs
- 280 Downloads
The heritability of genetic resistance of radiata pine against Fusarium circinatum was not clear. We demonstrated that there are at least 3 QTLs that could be involved in this resistance/susceptibility.
A genetic linkage map was developed for Pinus radiata, using Amplified Fragment Length Polymorphism (AFLP), Inter-Simple Sequence Repeat (ISSR), Selective Amplification of Microsatellite Polymorphic Loci (SAMPL), and Simple Sequence Repeat (SSR) molecular markers, based on a two-way pseudo-testcross strategy, using 86 individuals of a F1 full-sib family and 787 molecular markers for genotyping. Linkage analysis generated a map of medium to high density for each parent, with 1,060 and 1,258 cM for parents XO and XP, respectively. A total of 458 markers were mapped on 12 linkage groups (LG) in XO and XP, which equals the number of haploid chromosomes present in P. radiata. Analysis of quantitative trait loci (QTL) for resistance against pitch canker disease caused by Fusarium circinatum was made using Bayesian Information Criterion (BIC). In the XO parental map, two groups (LG-1 and LG-9) showed high probabilities for one or more QTLs. Only one group (LG-9) in the XP parental map showed probability for one or more QTLs. The results indicate that resistance to pitch canker is inherited from both parents. These results provide the basis for further studies focused on structure, evolution, and function of the P. radiata genome.
KeywordsRadiata pine Fusarium circinatum Linkage analysis Quantitative resistance Molecular markers
This work was financed by Genómica Forestal (CORFO, grant number 05CTE04-02) and a PhD grant from Comisión Nacional de Ciencia y Tecnología (23100216) to PM.
Conflict of interest
The authors declare that they have no conflict of interest.
- Alonso R, Bettucci L (2009) First report of the pitch canker fungus Fusarium circinatum affecting Pinus taeda seedlings in Uruguay. Australas Plant Dis Notes 4:91–92Google Scholar
- Beavis WD (1994) The power and deceit of QTL experiments: lessons from comparative QTL studies. In: Proceedings of the 49th annual corn and sorghum industry research conference. American Seed Trade Association, Washington, pp 250–266Google Scholar
- Beeche M, Gonzalez P, Ide S, Sandoval A, Murillo ME (2005) Informativo fitosanitario forestal. Servicio Agrícola y Ganadero 1:1–5Google Scholar
- Berry CR, Hepting GH (1959) Pitch canker of southern pines. USDA For. Pest Leaflet No. 35Google Scholar
- Dwinell LD, Adams D, Guerra-Santos JJ, Aquirre JRM (1998) Pitch canker disease of Pinus radiata. Paper 3.7.30 in offered paper abstracts—volume 3. In: Proceedings of the 7th international congress of plant pathology, Edinburgh, ScotlandGoogle Scholar
- Gonzalez G (2005) Resultados preliminares del estudio de Fusarium circinatum: conocimiento del patógeno y establecimiento de bases para su control. En: Resúmenes de la XX Silvotecna: Sanidad forestal en un mundo globalizado. Sesión no 13, Concepción, ChileGoogle Scholar
- Guerra-Santos J (1998) Pitch canker on Monterey pine in Mexico. In: Devey M, Matheson C, Gordon TR (eds) Current and potential impacts of pitch canker in radiata pine. Proceedings of the IMPACT monterey workshop, Monterey, California, 30 November to 3 December. CSIRO, Collingwood, Victoria, Australia, pp 58–61Google Scholar
- Hepting GH, Roth ER (1946) Pitch canker, a new disease of some southern pines. J For 44:742–774Google Scholar
- Jacobs A, Coutinho TA, Wingfield MJ, Ahumada R, Wingfield BD (2007) Characterization of the pitch canker fungus, Fusarium circinatum, from Chile. S Afr J Sci 103:253–257Google Scholar
- Lynch M, Walsh B (1998) Genetics and analysis of quantitative traits. Sinauer Associates Inc, SunderlandGoogle Scholar
- Mitchell G, Jones N, Coutinho T (2005) Alternatives to benomyl fungicide in controlling Fusarium circinatum: results from in vitro studies. Forest research shaw research centre, SAPPI, South Africa, pp 1–14 (Document 3)Google Scholar
- Plomion C, Chagné D, Pot D, Kumar S, Wilcox P, Burdon R, Prat D, Peterson D, Paiva J, Chaumeil P, Vendramin G, Sebastiani F, Nelson C, Echt C, Savolainen O, Kubisiak T, Cervera M, De María N, Islam-Faridi M (2007) In: Genome mapping and molecular breeding in plants, volume 7 forest trees. Chap 2, pp 29–92Google Scholar
- R Development Core Team (2011) R: a language and environment for statistical computing. Vienna, Austria: the R Foundation for Statistical Computing. ISBN: 3-900051-07-0. Available online at http://www.R-project.org/
- Rotella A (2005) Manejo del hongo Fusarium circinatum en viveros forestales. En: Resúmenes de la XX Silvotecna: Sanidad forestal en un mundo globalizado. Sesión no 12, Concepción, ChileGoogle Scholar
- SAS Institute Inc., (2000) SAS 9.1.3 Help and Documentation, Cary, NC: SAS Institute Inc.,Google Scholar
- Schweigkofler W, O`Donnell K, Garbelotto M (2004) Detection and quantification of airborne conidia of Fusarium circinatum, the causal agent of pine pitch canker, from two California sites by using a real-time PCR approach cobined with a simple spore trapping method. Appl Environ Microb 70:3512–3520CrossRefGoogle Scholar
- Semagn K, Bjornstad A, Ndjiondjop MN (2006) Principles, requirements and prospects of genetics mapping in plants. Afr J Biotechnol 5:2569–2587Google Scholar
- Wikler K, Gordon TR (2000) An initial assessment of genetic relationships among populations of Fusarium circinatum in different parts of the world. Can J Bot 78:709–717Google Scholar
- Wikler K, Gordon TR, Storer AJ, Wood DL (2003) Pitch Canker. Integrated pest management for home gardeners and landscape professionals. Pest Note 741707:1–5Google Scholar
- Wilcox PL, Richardson TE, Corbet GE, Ball RD, Lee JR, Djorovic A, Carson SD (2001) Framework linkage maps of Pinus radiata D. Don based on pseudotestcross markers. For Genet 8:109–117Google Scholar
- Wilcox PL, Cato S, McMillan L, Power M, Ball RD, Burdon RD, Echt CS (2004) Patterns of linkage disequilibrium in Pinus radiata. In: Plant and animal genome XII Conf, San Diego, pp W89. http://www.intlpag.org/12/abstracts/W22_PAG12_89.html
- Xu S (2003) Theoretical basis of the Beavis effect. Genetics 165:2259–2268Google Scholar