Trees

, Volume 27, Issue 1, pp 161–169 | Cite as

Foliar-applied Dormex™ or thiourea-enhanced proline and biogenic amine contents and hastened breaking bud dormancy in “Ain Shemer” apple trees

Original Paper
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Abstract

The role of hydrogen cyanamide (Dormex™) or thiourea in the acceleration of dormancy breaking in buds of “Ain Shemer” apple (Malus sylvestris, Mill.) trees, and their effects on metabolic changes in the contents of proline and biogenic amines (BAs; tyramine, tryptamine, histamine, methylbutylamine and serotonin) in buds during bud break were assessed. The efficiency of bud break by these compounds was noticed to varying degrees. Breaking bud dormancy was correlated with the early date of bud break, the short duration of flowering, the high percentages of bud break and fruit set, and the high contents of proline and BAs. This finding was positively reflected in the tree’s yield. Dormex™ was found to be more effective than thiourea; therefore, we recommend using Dormex™ for early bud break, short period of flowering and high percentages of bud break and fruit set by regulating the contents of proline and BAs in buds.

Keywords

“Ain Shemer” apple Dormancy Dormex™ Thiourea Bud break Biogenic amines Proline 
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References

  1. Alcázar R, Marco F, Cuevas JC (2006) Involvement of polyamines in plant response to abiotic stress. Biotech Lett 28:1867–1876CrossRefGoogle Scholar
  2. Arora R, Rowland LJ, Tanino K (2003) Induction and release of bud dormancy in woody perennials: a science comes of age. HortSci 38:911–921Google Scholar
  3. Bai C, Reilly CC, Wood BW (2007) Nickel deficiency affects nitrogenous forms and urease activity in spring xylem sap of pecan. J Amer Soc Hort Sci 132:302–309Google Scholar
  4. Bais HP, Ravishankar GA (2002) Role of polyamines in the ontogeny of plants and their biotechnological applications. Plant Cell Tissue Organ Cult 69:1–34CrossRefGoogle Scholar
  5. Bajji M, Hamdi MM, Gastiny F, Rojas-Beltran JA, Jardin P (2007) Catalase inhibition accelerates dormancy release and sprouting in potato (Solanum tuberosum L.) tubers. Biotech Agron Soc Environ 11:121–131Google Scholar
  6. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  7. Ben Mohamed H, Vadel MA, Khemira H (2010) Estimation of chilling requirement and effect of hydrogen cyanamide on budbreak and fruit characteristics of ‘Superior Seedless’ table grape cultivated in a mild winter climate. Pak J Bot 42:1761–1770Google Scholar
  8. Ben Mohamed H, Vadel AM, Geuns JMC, Khemira H (2012) Effects of hydrogen cyanamide on antioxidant enzymes’ activity, proline and polyamine contents during bud dormancy release in Superior Seedless grapevine buds. Acta Physiol Plant 34:429–437CrossRefGoogle Scholar
  9. Bohlenius H, Huang T, Charonnel-Campaa L, Brunner AM, Jansson S, Srauss SH, Nilsson O (2006) CO/FT regulatory module controls timing of flowering and seasonal growth cessation in trees. Sci 31:140–141Google Scholar
  10. Bokern M, Witte L, Wray V, Nimtz M, Meurer-Grimes B (1995) Trisubstituted hydroxycinnamic acid spermidines from Quercus dentata pollen. Phytochem 39:1371–1375CrossRefGoogle Scholar
  11. Bound SA, Jones KM (2004) Hydrogen cyanamide impacts on flowering, crop load, and fruit quality of red ‘Fuji’ apple (Malus domestica). New Zea J Crop Hort Sci 32:227–234CrossRefGoogle Scholar
  12. Chen C, Dickman MB (2005) Proline suppresses apoptosis in the fungal pathogen (Colletotrichum trifolii). Proc Natl Acad Sci USA 102:3459–3464PubMedCrossRefGoogle Scholar
  13. Coruzzi GM, Zhou L (2001) Carbon and nitrogen sensing and signaling in plants: emerging ‘matrix effects’. Curr Opin Plant Biol 4:247–253PubMedCrossRefGoogle Scholar
  14. Couturier J, de Fay E, Fitz M, Wipf D, Blaudez D, Chalot M (2010) PtAAP11, a high affinity amino acid transporter specifically expressed in differentiating xylem cells of poplar. J Exp Bot 26:1–12Google Scholar
  15. Durzan DJ (1989) Nitrogenous extractives. Amino acids, proteins, and nucleic acids. In: Rowe JW (ed) Natural products of woody plants. 1. Chemicals extraneous to the lignocellulosic cell wall. New York, Springer, pp 179–200Google Scholar
  16. Durzan DJ (2009) Transamidination-based recycling of the respiratory inhibitor [1-14C]-guanidinoacetic acid to 14C-glycine in white spruce shoot primordia entering winter dormancy (Picea glauca Moench Voss). Trees Struct Funct 24:335–341Google Scholar
  17. Durzan DJ (2010) Arginine and the shade tolerance of white spruce saplings entering winter dormancy. J For Sci 56:77–83Google Scholar
  18. Egea J, Ortega E, Martynez-Gomez P, Dicenta F (2003) Chilling and heat requirements of almond cultivars for flowering. Environ Exp Bot 50:79–85CrossRefGoogle Scholar
  19. Engin H, Gokbayrak Z, Dardeniz A (2010) Effect of hydrogen cyanamide on the floral morphogenesis of kiwi fruit buds. Chil J Agric Res 70:503–509Google Scholar
  20. Ennajeh M, Vadel AM, Khemira H, Ben Mimoun M, Hellali R (2006) Defense mechanisms against water deficit in two olive (Olea europaea L.) cultivars ‘Meski’ and ‘Chemlali’. J Hort Sci Biotech 81:99–104Google Scholar
  21. Espinosa-Ruiz A, Saxena S, Schmidt J, Mellerowicz E, Miskolczi P, Bako L, Bhalerao RP (2004) Differential stage-specific regulation of cyclin-dependent kinases during cambial dormancy in hybrid aspen. Plant J 38:603–615PubMedCrossRefGoogle Scholar
  22. Faiza B, Halima ZK, Nour-Eddine K (2011) Physiological responses of salt stress and osmoprotection with proline in two strains of lactococci isolated from camel’s milk in Southern Algeria. Afr J Biotech 10:19429–19435Google Scholar
  23. Flores HE, Galston AW (1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol 69:701–706PubMedCrossRefGoogle Scholar
  24. Foott JH (1987) The effect of hydrogen cyanamide on breaking endodormancy of mid-chilling apple. Acta Hort 329:268–270Google Scholar
  25. George AP, Nissen RJ (1990) Effect of hydrogen cyanamide on yield, growth and dormancy release of table grapes in subtropical Australia. Acta Hort 279:427–436Google Scholar
  26. Germchi S, Behroozi FG, Badri S (2011) Effect of thiourea on dormancy breaking and yield of potato (Solanum Tuberosum L.) Minitubers Marfona cv. in Greenhouse. Int Conf Environ Agric Eng 15:19–24Google Scholar
  27. Geuns JMC, Orriach ML, Swennen R, Zhu G, Panis B, Compernolle F, Auweraer MV (2006) Simultaneous liquid chromatography determination of polyamines and arylalkyl monoamines. Analyt Biochem 354:127–131PubMedCrossRefGoogle Scholar
  28. Gomez KA, Gomez AA (1984) Statistical analysis procedure of agricultural research. Wiley, New York, pp 25–30Google Scholar
  29. Hamilton EW III, Heckathorn SA (2001) Mitochondrial adaptations to NaCl. Complex I is protected by anti-oxidants and small heat shock proteins, whereas complex II is protected by proline and betaine. Plant Physiol 126:1266–1274PubMedCrossRefGoogle Scholar
  30. Hanson GR, Venturelli PJ, Fleckenstein AE (2005) Drugs and society, 9th edn. Jones and Bartlett Publishers, Massachusetts. Accessed 2011–04–19Google Scholar
  31. Heide OM, Prestrud AK (2005) Low temperature, but not photoperiod controls growth cessation and dormancy induction and release in apple and pear. Tree Physiol 25:109–114PubMedCrossRefGoogle Scholar
  32. Hoque A, Banu NA, Okuma E, Amako K, Nakamura Y, Shimoishi Y, Murata Y (2007) Exogenous proline and glycinebetaine increase NaCl-induced ascorbate–glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells. J Plant Physiol 164:1457–1468PubMedCrossRefGoogle Scholar
  33. Hossain MDA, Ashrafuzzaman MD, Ismail MR (2011) Salinity triggers proline synthesis in peanut leaves. Maejo Int J Sci Technol 5:159–168Google Scholar
  34. Judd MJ, Meyer DH, Meekings JS, Richardson AC, Walton EF (2010) An FTIR study of the induction and release of kiwifruit buds from dormancy. J Sci Food Agric 90:1071–1080PubMedGoogle Scholar
  35. Kakkar RK, Sawhney VK (2002) Polyamine research in plants—a changing perspective. Physiol Plant 116:281–292CrossRefGoogle Scholar
  36. Kaur G, Kumar S, Thakur P, Malik JA, Bhandhari K, Sharma KD, Nayyar H (2011) Involvement of proline in response of chickpea (Cicer arietinum L.) to chilling stress at reproductive stage. Sci Hort 128:174–181CrossRefGoogle Scholar
  37. Kavi KPB, Sangam S, Amrutha RN, Sri Laxmi P, Naidu KR, Rao KRSS, Rao S, Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438Google Scholar
  38. Kusano T, Berberich T, Tateda Y, Takahashi Y (2008) Polyamines: essential factors for growth and survival. Planta 228:367–381PubMedCrossRefGoogle Scholar
  39. Li C, Junttila O, Ernstsen A, Heino P, Palya ET (2003) Photoperiodic control of growth, cold acclimation and dormancy development in silver birch (Betula pendula) ecotypes. Physiol Plant 117:206–212CrossRefGoogle Scholar
  40. Linsley-Noakes GC (1989) Improving flowering of kiwifruit in climatically marginal areas using hydrogen cyanamide. Sci Hort 38:247–259CrossRefGoogle Scholar
  41. Luna V, Soriano MD, Bottini R, Sheng C, Pharis RP (1993) Levels of endogenous gibberellin, abscisic acid, indole acetic acid and naringenin during dormancy of peach flower buds. Acta Hort 329:265–267Google Scholar
  42. Millard P, Wendler R, Hepburn A, Smith A (1998) Variations in the amino acid composition of xylem sap of Betula pendula Roth. Trees due to remobilization of stored N in the spring. Plant Cell Environ 21:715–722CrossRefGoogle Scholar
  43. Pereira N, Oliveira CM, Mota M, Sousa RM (2010) Evaluation of five dormancy breaking agent to induce synchronized flowering in “Roche” pear. Acta Hort (ISHS) 909:423–428Google Scholar
  44. Pérez FJ, Vergara R, Or E (2009) On the mechanism of dormancy release in grapevine buds: a comparative study between hydrogen cyanamide and sodium azide. Plant Growth Regul 59:145–152CrossRefGoogle Scholar
  45. Quittenden LJ, Davies NW, Smith JA, Molesworth PP, Tivendale ND, Ross JJ (2009) Auxin biosynthesis in pea: characterization of the tryptamine pathway. Plant Physiol 151:1130–1138PubMedCrossRefGoogle Scholar
  46. Rohde A, Prinsen E, De Rycke R, Engler G, Van Montagu M, Boerjan W (2002) PtABI3 impinges on the growth and differentiation of embryonic leaves during bud set in poplar. Plant Cell 14:1885–1901PubMedCrossRefGoogle Scholar
  47. Sabry GH, El-Helw HA, Abd El-Rahman AS (2011) A study on using jasmine oil as a breaking bud dormancy for Flame Seedless grapevines. Rep Opin 3:48–56Google Scholar
  48. Seif El-Yazal MA, Rady MM (2012) Changes in nitrogen and polyamines during breaking bud dormancy in “Anna” apple trees with foliar application of some compounds. Sci Hort 136:75–80CrossRefGoogle Scholar
  49. Shetty K, Wahlqvist ML (2004) A model for the role of the praline-linked pentose-phosphate pathway in phenolic phytochemical biosynthesis and mechanism of action for human health and environmental application. Asia Pac J Clin Nutr 13:1–24PubMedGoogle Scholar
  50. Simmonds JA, Simpson GM (1972) Regulation of Krebs cycle and pentose phosphate pathway activities in the control of dormancy of Avena fatua. Can J Bot 50:1041–1048CrossRefGoogle Scholar
  51. Sivakumar P, Sharmila P, Saradhi PP (2000) Proline alleviates salt-stress-induced enhancement in ribulose-1,5-bisphosphate oxygenase activity. Biochem Biophys Res Commun 279:512–515PubMedCrossRefGoogle Scholar
  52. Skopelitis DS, Paranychianakis NV, Paschalidis KA, Pliakonis ED, Delis ID, Yakoumakis DI, Kouvarakis A, Papadakis AK, Stephanou EG, Roubelakis- Angelakis KA (2006) Abiotic stress generates ROS that signal expression of anionic glutamate dehydrogenases to form glutamate for proline synthesis in tobacco and grapevine. Plant Cell 18:2767–2781PubMedCrossRefGoogle Scholar
  53. Subha-Drabandhu S (1995) Induction of bud break in apple trees that received insufficient chilling by hydrogen cyanamide. Acta Hort 409:171–178Google Scholar
  54. Takano K, Higashi R, Okada J, Mukaiyama A, Tadokoro T, Koga Y, Kanaya S (2009) Proline effect on the thermostability and slow unfolding of a hyperthermophilic protein. J Biochem 145:79–85PubMedCrossRefGoogle Scholar
  55. Tian WN, Braunstein LD, Pang J, Stuhlmeier KM, Xi QC, Tian X, Stanton RC (1998) Importance of glucose-6-phosphate-dehydrogenase activity for cell growth. J Biol Chem 273:10609–10617PubMedCrossRefGoogle Scholar
  56. Tieman D, Taylor M, Schauer N (2006) Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde. PNAS 103:8287–8292PubMedCrossRefGoogle Scholar
  57. Trejo-Martínez MA, Orozco JA, Almaguer-Vargas G, Carvajal-Millán E, Gardea AA (2009) Metabolic activity of low chilling grapevine buds forced to break. Thermochim Acta 481:28–31CrossRefGoogle Scholar
  58. Ud-Din J, Ullahkhan S, Gurmani A (2012) Physiological and biochemical constituents as predictive appreciation for selection of drought tolerant cultivars in wheat (Triticum aestivum L.). J Chem Soc Pak 34:151–155Google Scholar
  59. Vergara R, Pérez FJ (2010) Similarities between natural and chemically induced bud-endodormancy release in grapevine (Vitis vinifera L.). Sci Hort 125:648–653CrossRefGoogle Scholar
  60. Walters DR (2003) Polyamines in plant disease. Phytochem 64:97–107CrossRefGoogle Scholar
  61. Walton EF, Clark CJ, Boldingh HL (1991) Effect of hydrogen cyanamide on amino acid profiles in kiwifruit buds during budbreak. Plant Physiol 97:1256–1259PubMedCrossRefGoogle Scholar
  62. Walton EF, Wu RM, Richardson AC, Davy M, Hellens RP, Thodey K, Janssen BJ, Gleave AP, Rae GM, Wood M, Schaffer RJ (2009) A rapid transcriptional activation is induced by the dormancy-breaking chemical hydrogen cyanamide in kiwifruit (Actinidia deliciosa) buds. J Exp Bot 60:3835–3848PubMedCrossRefGoogle Scholar
  63. Wang SY, Jiao HJ, Faust M (1991) Changes in ascorbate, glutathione, and related enzyme activities during thidiazuron-induced bud break of apple. Physiol Plant 82:231–236CrossRefGoogle Scholar
  64. Wang ZQ, Yung YZ, Ou JQ, Lin QH, Zhang CF (2007) Glutamine synthetase and glutamate dehydrogenase contribute differentially to proline accumulation in leaves of wheat (Triticum aestivum) seedlings exposed to different salinity. J Plant Physiol 164:695–701PubMedCrossRefGoogle Scholar
  65. Wipf D, Ludewig U, Tegeder M, Rentsch D, Koch W, Frommer WB (2002) Conservation of amino acid transporters in fungi, plants and animals. Trends Biochem Sci 27:139–147PubMedCrossRefGoogle Scholar
  66. Xu HW, Lu Y, Tong SY, Song FB (2011) Lipid peroxidation, antioxidant enzyme activity and osmotic adjustment changes in husk leaves of maize in black soils region of Northeast China. Afr J Agric Res 6:3098–3102Google Scholar
  67. Yamane H, Kashiwa Y, Ooka T, Tao R, Yonemori K (2008) Suppression subtractive hybridization and differential screening reveals endodormancy-associated expression of an SVP/AGL24-type MADS-box gene in lateral vegetative buds of Japanese apricot. J Am Soc Hort Sci 133:708–716Google Scholar
  68. Yang YS, Chang MT, Shen BK (1990) The effect of calcium cyanamide on bud break retranslocation of accumulated 14C-assimilates and changes of nitrogen in grapevines in Taiwan. Acta Hort 279:409–425Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Botany Department, Faculty of AgricultureFayoum UniversityFayoumEgypt

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