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Trees

, Volume 27, Issue 1, pp 149–159 | Cite as

Odors of Norway spruce (Picea abies L.) seedlings: differences due to age and chemotype

  • Astrid Kännaste
  • Tao Zhao
  • Anders Lindström
  • Eva Stattin
  • Bo Långström
  • Anna-Karin Borg-KarlsonEmail author
Original Paper

Abstract

Small conifer seedlings (mini-seedlings) are less damaged by the large pine weevil Hylobius abietis (L.) (Coleoptera: Curculionidae) compared to conventional seedlings. Chemical difference between the seedling types is one possible explanation for this phenomenon. In the present paper, the emissions of volatile organic compounds (VOC) of 7- to 43-week-old Norway spruce [Picea abies (L.) Karst.] seedlings were analyzed. Collection and identification of the volatiles was made by solid phase micro-extraction and gas chromatography mass spectrometry (SPME–GC–MS). The enantiomers of α-pinene and limonene were separated in a two-dimensional GC (2D-GC). Most of the seedlings represented either a limonene- or a bornyl acetate-chemotype. Only minor changes in the volatile composition of the two types of seedlings were found during the first year. Age-related changes, however, were found in the volatiles released by wounded phloem where green leaf volatiles (GLVs) and borneol were the dominated VOC for young seedling. The attractive compound for the pine weevil, α-pinene, was first detected in the phloem emissions of 18- to 22-week-old seedlings. Different storage conditions of the seedlings during the winter/early spring-phase influenced the volatile composition in the phloem. High amount of GLVs was characteristic for the 43-week-old seedlings stored in naturally changing outdoor temperature, but not present in the seedlings winter-stored at a constant temperature of −4 °C. The possible role of these observed differences in odor emissions between seedlings of different age and physiological status for the feeding preferences of the large pine weevil is discussed.

Keywords

Picea abies (L.) Karst. Mini-seedling Volatile emission Chemotype Age Green leaf volatiles Estragol 

Notes

Acknowledgments

This study is financed by FORMAS (The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning) and SSF (Swedish Foundation for Strategic Research). We also thank EU-Mobilitas (to AKBK) and Archimedes foundation for a stipend to Astrid Kännaste.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Astrid Kännaste
    • 1
    • 4
  • Tao Zhao
    • 1
  • Anders Lindström
    • 2
  • Eva Stattin
    • 2
  • Bo Långström
    • 3
  • Anna-Karin Borg-Karlson
    • 1
    • 5
    Email author
  1. 1.Ecological Chemistry Group, Department of Chemistry, Royal Institute of TechnologyStockholmSweden
  2. 2.School of Industrial Technology and ManagementDalarna UniversityBorlängeSweden
  3. 3. Department of EcologySwedish University of Agricultural SciencesUppsalaSweden
  4. 4.Institute of Agricultural and Environmental Sciences, Department of Plant PhysiologyEstonian University of Life SciencesTartuEstonia
  5. 5.Institute of Technology, Division of Organic ChemistryTartu UniversityTartuEstonia

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