Trees

, Volume 26, Issue 5, pp 1565–1576 | Cite as

Chlorophyll fluorescence of the trunk rather than leaves indicates visual vitality in Eucalyptus saligna

  • Denise Johnstone
  • Michael Tausz
  • Gregory Moore
  • Marc Nicolas
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Leaf chlorophyll fluorescence has been used to assess physiological stress effects in trees for at least 30 years. This paper describes a novel method for indicating tree vitality in Eucalyptus saligna Sm using bark chlorophyll fluorescence. A visual vitality index was successfully verified by comparing it with growth measurements such as total leaf area and above ground biomass. Bark and leaf chlorophyll fluorescence were then compared with the visual vitality index in spring, summer and autumn. The relationship between leaf chlorophyll fluorescence and the visual vitality index was weak, even with the one parameter that did show a relationship. There was good evidence for a statistical relationship between bark chlorophyll fluorescence and visual vitality, which may become a useful tool for tree vitality assessments in this species and possibly other tree species.

Keywords

Bark Photosynthesis Chlorophyll fluorescence Eucalyptus Stress physiology Tree growth 
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References

  1. Bjorkman O, Demmig B (1987) Photon yield of O-2 evolution and chlorophyll fluorescence characteristics at 77-K among vascular plants of diverse origins. Planta 170:489–504CrossRefGoogle Scholar
  2. Buhler O, Kristoffersen P, Larson P (2007) Growth of street trees in Copenhagen with emphasis on the effect of different establishment concepts. Arboric Urban For 33:330–337Google Scholar
  3. Bukhov NG, Carpentier R (2004) Effects of water stress on photosynthetic efficiency of plants. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a Fluorescence. A signature of Photosynthesis. Springer, Dordrecht, pp 623–635Google Scholar
  4. Bureau of Meteorology Australia (2008) http://www.bom.gov.au/. Accesssed 13 Feb 2008
  5. Bussotti F (2004) Assessment of stress conditions in Quercus ilex L. leaves by OJIP chlorophyll a fluorescence analysis. Plant Biosyst 138:101–109CrossRefGoogle Scholar
  6. Bussotti F, Strasser RJ, Schaub M (2007) Photosynthetic behavior of woody species under high ozone exposure probed with the JIP-test: a review. Environ Pollut 147:430–437PubMedCrossRefGoogle Scholar
  7. Contran N, Poletti E, Manning W, Tagliaferro F (2009) Ozone sensitivity and ethylenediurea protection in ash trees assessed by JIP chlorophyll a fluorescence transient analysis. Photosynthetica 47:68–78CrossRefGoogle Scholar
  8. Coops NC, Stone C, Culvenor DS, Chisholm L (2004) Assessment of crown condition in eucalypt vegetation by remotely sensed optical indices. J Environ Qual 33:956–964PubMedCrossRefGoogle Scholar
  9. Cunningham SC, Read J, Baker PJ, Mac Nally R (2007) Quantitative assessment of stand condition and its relationship to physiological stress in stands of Eucalyptus camaldulensis (Myrtaceae). Aust J Bot 55:692–699CrossRefGoogle Scholar
  10. Damesin C (2003) Respiration and photosynthesis characteristics of current-year stems of Fagus sylvatica: from the seasonal pattern to an annual balance. New Phytol 158:465–475CrossRefGoogle Scholar
  11. Dobbertin M (2005) Tree growth as indicator of tree vitality and of tree reaction to environmental stress: a review. Eur J For Res 124:319–333CrossRefGoogle Scholar
  12. Epron D, Dreyer E, Breda N (1992) Photosynthesis of oak trees [Quercus petraea (Matt) Liebl] during drought under field conditions—diurnal course of net CO2 assimilation and photochemical efficiency of photosystem-II. Plant Cell Environ 15:809–820CrossRefGoogle Scholar
  13. Eyles A, Pinkard EA, O’Grady AP, Worledge D, Warren CR (2009) Role of corticular photosynthesis following defoliation in Eucalyptus globulus. Plant Cell Environ 32:1004–1014PubMedCrossRefGoogle Scholar
  14. Flexas J, Bota J, Escalona JM, Sampol B, Medrano H (2002) Effects of drought on photosynthesis in grapevines under field conditions: an evaluation of stomatal and mesophyll limitations. Funct Plant Biol 29:461–471CrossRefGoogle Scholar
  15. Fostad O, Pedersen PA (1997) Vitality, variation and causes of decline of trees in Oslo center (Norway). J Arboric 23:155–165Google Scholar
  16. Gerosa G, Marzuoli R, Bussotti F, Pancrazi M, Ballarin-Denti A (2003) Ozone sensitivity of Fagus sylvatica and Fraxinus excelsior young trees in relation to leaf structure and foliar ozone uptake. Environ Pollut 125:91–98PubMedCrossRefGoogle Scholar
  17. Govindjee (2004) Chlorophyll a fluorescence: a bit of basics and history. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence. A signature of photosynthesis. Springer, Dordrecht, pp 1–41Google Scholar
  18. Gratani L, Crescente M, Petruzzi M (2000) Relationship between leaf life-span and photosynthetic activity of Quercus ilex in polluted urban areas (Rome). Environ Pollut 110:19–28PubMedCrossRefGoogle Scholar
  19. Grimes R (1978) Crown assessment of natural spotted gum (Eucalyptus maculata), ironbark (Eucalyptus fibrosa, Eucalyptus drepanophylla) forest. Technical paper No. 7. Queensland Department of Forestry 7:1–16Google Scholar
  20. Hansatech (2006) Operations manual handy PEA pocket PEA and PEA plus software. Hansatech Instruments, NorfolkGoogle Scholar
  21. Hunt R (2003) Growth analysis, individual plants. In: Thomas B, Murphy DJ, Murray BG (eds) Encyclopaedia of applied plant sciences, vol 2. Academic Press, London, pp 579–588Google Scholar
  22. Jiang H, Howell G, Flore J (1999) Efficacy of chlorophyll fluorescence as a viability test for freeze-stressed woody grape tissue. Can J Plant Sci 79:401–410CrossRefGoogle Scholar
  23. Johnson G, Young A, Scholes J, Horton P (1993) The dissipation of excess excitation energy in British plant species. Plant Cell Environ 16:673–679CrossRefGoogle Scholar
  24. Kozlowski TT, Kramer PJ, Pallardy SG (1991) The physiological ecology of woody plants. Academic Press, Inc, San DiegoGoogle Scholar
  25. Krause GH, Weis E (1984) Chlorophyll fluorescence as a tool in plant physiology. Photosynth Res 5:139–157CrossRefGoogle Scholar
  26. Larcher W (2003) Physiological plant ecology: ecophysiology and stress physiology of functional groups. Springer, BerlinGoogle Scholar
  27. Lindenmayer D, Norton T, Tanton M (1990) Differences between wildfire and clearfelling on the structure of montane ash forests of Victoria and their implications for fauna dependent on tree hollows. Aust For 53:61–68Google Scholar
  28. Maki DS, Colombo SJ (2001) Early detection of the effects of warm storage on conifer seedlings using physiological tests. For Ecol Manag 154:237–249CrossRefGoogle Scholar
  29. Manetas Y (2004) Probing corticular photosynthesis through in vivo chlorophyll fluorescence measurements: evidence that high internal CO2 levels suppress electron flow and increase the risk of photoinhibition. Physiol Plant 120:509–517PubMedCrossRefGoogle Scholar
  30. Martin RAU, Burgman MA, Minchin PR (2001) Spatial analysis of eucalypt dieback at Coranderrk, Australia. Appl Veg Sci 4:257–266CrossRefGoogle Scholar
  31. Martinez-Trinidad T, Watson WT, Arnold MA, Lombardini L, Appel DN (2010) Comparing various techniques to measure tree vitality of live oaks. Urban For Urban Green 9:199–203CrossRefGoogle Scholar
  32. Martínez-Trinidad T, Watson W, Arnold M, Lombardini L, Appel D (2009) Carbohydrate injections as a potential option to improve growth and vitality of live oaks. Arboric Urban For 35:142–147Google Scholar
  33. Matyssek R, Günthardt-Goerg MS, Keller T, Scheidegger C (1991) Impairment of gas exchange and structure in birch leaves (Betula pendula) caused by low ozone concentrations. Trees-Struct Funct 5:5–13CrossRefGoogle Scholar
  34. Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659PubMedCrossRefGoogle Scholar
  35. Mussche S, Samson R, Nachtergale L, De Schrijver A, Lemeur R, Lust N (2001) A comparison of optical and direct methods for monitoring the seasonal dynamics of leaf area index in deciduous forests. Silva Fenn 35:373–384Google Scholar
  36. Pandey J, Pandey U (1994) Evaluation of air pollution phytotoxicity in a seasonally dry tropical urban environment. Environ Monit Assess 33:195–213CrossRefGoogle Scholar
  37. Percival GC (2004) Evaluation of physiological tests as predictors of young tree establishment and growth. J Arboric 30:80–91Google Scholar
  38. Percival G (2005a) The use of chlorophyll fluorescence to identify chemical and environmental stress in leaf tissue of three oak (Quercus) species. J Arboric 31:215–227Google Scholar
  39. Percival GC (2005b) Identifying tree decline using “fluorescence fingerprinting”. International Society of Arboriculture Australia Chapter, HobartGoogle Scholar
  40. Percival GC, Fraser GA (2001) Measurement of the salinity and freezing tolerance of Crataegus genotypes using chlorophyll fluorescence. J Arboric 27:233–245Google Scholar
  41. Percival G, Keary I (2008) The influence of nitrogen fertilization on waterlogging stresses in Fagus sylvatica L. and Quercus robur L. Arboric Urban For 34:29Google Scholar
  42. Percival GC, Fraser GA, Oxenham G (2003) Foliar salt tolerance of Acer genotypes using chlorophyll fluorescence. J Arboric 29:61–65Google Scholar
  43. Pfanz H (2008) Bark photosynthesis. Trees-Struct Funct 22:137–138CrossRefGoogle Scholar
  44. Pfanz H, Aschan G, Langenfeld-Heyser R, Wittmann C, Loose M (2002) Ecology and ecophysiology of tree stems: corticular and wood photosynthesis. Naturwissenschaften 89:147–162PubMedCrossRefGoogle Scholar
  45. Philip E, Noor Azlin Y (2005) Measurement of soil compaction tolerance of Lagestromia speciosa (L.) Pers. using chlorophyll fluorescence. Urban For Urban Green 3:203–208CrossRefGoogle Scholar
  46. Poorter H, Niinemets Ü, Poorter L, Wright IJ, Villar R (2009) Causes and consequences of variation in leaf mass per area (LMA): a meta analysis. New Phytol 182:565–588PubMedCrossRefGoogle Scholar
  47. Posch S, Bennett L (2009) Photosynthesis, photochemistry and antioxidative defence in response to two drought severities and with re-watering in Allocasuarina luehmannii. Plant Biol 11:83–93PubMedCrossRefGoogle Scholar
  48. Pukacki PM, Kamiska-Rozek E (2005) Effect of drought stress on chlorophyll a fluorescence and electrical admittance of shoots in Norway spruce seedlings. Trees-Struct Funct 19:539–544CrossRefGoogle Scholar
  49. Rasband W (2008) ImageJ version 1.40 g. Image processing and analysis in Java USA National Institutes of Health http://rsbweb.nih.gov/ij/. Retrieved 29 August 2008
  50. Redfern DB, Boswell RC (2004) Assessment of crown condition in forest trees: comparison of methods, sources of variation and observer bias. For Ecol Manag 188:149–160CrossRefGoogle Scholar
  51. Repo T, Kalliokoski T, Domisch T, Lehto T, Mannerkoski H, Sutinen S, Finér L (2005) Effects of timing of soil frost thawing on Scots pine. Tree Physiol 25:1053PubMedCrossRefGoogle Scholar
  52. Rodríguez-Calcerrada J, Pardos JA, Gil L, Reich PB, Aranda I (2008) Light response in seedlings of a temperate (Quercus petraea) and a sub-Mediterranean species (Quercus pyrenaica): contrasting ecological strategies as potential keys to regeneration performance in mixed marginal populations. Plant Ecol 195:273–285CrossRefGoogle Scholar
  53. Strasser RJ, Stirbet AD (2001) Estimation of the energetic connectivity of PS II centres in plants using the fluorescence rise OJIP: fitting of experimental data to three different PS II models. Math Comput Simulat 56:451–462CrossRefGoogle Scholar
  54. Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence. A signature of photosynthesis. Springer, Dordrecht, pp 321–362Google Scholar
  55. Susplugas S, Srivastava A, Strasser RJ (2000) Changes in the photosynthetic activities during several stages of vegetative growth of Spirodela polyrhiza: effect of chromate. J Plant Physiol 157:503–512CrossRefGoogle Scholar
  56. Tausz M, Warren CR, Adams MA (2005) Is the bark of shining gum (Eucalyptus nitens) a sun or a shade leaf? Trees-Struct Funct 19:415–421CrossRefGoogle Scholar
  57. Torelli N, Shortle W, Cufar K, Ferlin F, Smith K (1999) Detecting changes in tree health and productivity of silver fir in Slovenia. Eur J For Pathol 29:189–197CrossRefGoogle Scholar
  58. Valladares F, Dobarro I, Sánchez-Gómez D, Pearcy RW (2005) Photoinhibition and drought in Mediterranean woody saplings: scaling effects and interactions in sun and shade phenotypes. J Exp Bot 56:483PubMedCrossRefGoogle Scholar
  59. Weng JH, Chen YN, Liao TS (2006) Relationships between chlorophyll fluorescence parameters and photochemical reflectance index of tree species adapted to different temperature regimes. Funct Plant Biol 33:241–246CrossRefGoogle Scholar
  60. White DA, Turner NC, Galbraith JH (2000) Leaf water relations and stomatal behavior of four allopatric Eucalyptus species planted in Mediterranean southwestern Australia. Tree Physiol 20:1157–1165PubMedCrossRefGoogle Scholar
  61. Wild A, Schmitt V (1995) Diagnosis of damage to Norway spruce (Picea abies) through biochemical criteria. Physiol Plant 93:375–382CrossRefGoogle Scholar
  62. Wittmann C, Pfanz H (2007) Temperature dependency of bark photosynthesis in beech (Fagus sylvatica L.) and birch (Betula pendula Roth.) trees. J Exp Bot 58:4293–4306PubMedCrossRefGoogle Scholar
  63. Wittmann C, Pfanz H (2008) Antitranspirant functions of stem periderms and their influence on corticular photosynthesis under drought stress. Trees-Struct Funct 22:187–196CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Denise Johnstone
    • 1
  • Michael Tausz
    • 2
  • Gregory Moore
    • 1
  • Marc Nicolas
    • 3
  1. 1.Department of Resource Management and GeographyUniversity of MelbourneRichmondAustralia
  2. 2.Department of Forest and Ecosystem ScienceUniversity of MelbourneCreswickAustralia
  3. 3.Department Agriculture and Food SystemsUniversity of MelbourneParkvilleAustralia

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