, Volume 25, Issue 4, pp 577–584 | Cite as

The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance

  • Md Imran Khan
  • Naseem Ahmad
  • Mohammad AnisEmail author
Original Paper


A valuable tropical tree, Salix tetrasperma Roxb. commonly known as Indian willow has been investigated for its in vitro regeneration potential using nodal explants obtained from a 30-year-old elite tree. Agar-solidified Woody Plant Medium (WPM) containing different concentrations of Plant Growth Regulators (PGRs) was used in the study. Shoot induction response was best on WPM supplemented with 6-benzyladenine (5.0 μM) where 90% explants responded with an average shoot number (4.40 ± 0.50) and shoot length (0.92 ± 0.04) after 6 weeks of culture. However, multiplication and elongation was best recorded when BA (5.0 μM) treated shoot clusters were transferred to WPM containing BA (1.0 μM) + NAA (0.5 μM) where 18.40 ± 0.92 well-grown healthy shoots with an average shoot length of 5.30 ± 0.43 cm were obtained on completion of 12 weeks culture period. In vitro rooting of shoots was best achieved in half-strength WPM containing 0.5 μM IBA. Well-rooted plantlets were successfully hardened off and acclimatized in plastic cups containing sterile Soilrite. These plantlets were then transferred to pots containing normal garden soil followed by transfer to greenhouse and ultimately to field under full sun.


Micropropagation Salix tetrasperma Roxb. Shoot induction Shoot multiplication In vitro rooting 





α Naphthalene acetic acid


Woody plant medium


Plant growth regulators



Financial assistance to Md Imran Khan in the form of JRF (NET) by Council of Scientific and Industrial Research (CSIR), Govt. of India, New Delhi is gratefully acknowledged. Research support provided by the Department of Science and Technology (DST) and University Grants Commission (UGC) Govt. of India, New Delhi, in the form of DST-FIST (2005) and UGC-SAP (2009) Programmes is duly acknowledged.

Supplementary material

468_2010_534_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 24 kb)


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Plant Production, College of Food and Agriculture ScienceKing Saud UniversityRiyadhSaudi Arabia

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