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Trees

, Volume 25, Issue 4, pp 577–584 | Cite as

The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance

  • Md Imran Khan
  • Naseem Ahmad
  • Mohammad AnisEmail author
Original Paper

Abstract

A valuable tropical tree, Salix tetrasperma Roxb. commonly known as Indian willow has been investigated for its in vitro regeneration potential using nodal explants obtained from a 30-year-old elite tree. Agar-solidified Woody Plant Medium (WPM) containing different concentrations of Plant Growth Regulators (PGRs) was used in the study. Shoot induction response was best on WPM supplemented with 6-benzyladenine (5.0 μM) where 90% explants responded with an average shoot number (4.40 ± 0.50) and shoot length (0.92 ± 0.04) after 6 weeks of culture. However, multiplication and elongation was best recorded when BA (5.0 μM) treated shoot clusters were transferred to WPM containing BA (1.0 μM) + NAA (0.5 μM) where 18.40 ± 0.92 well-grown healthy shoots with an average shoot length of 5.30 ± 0.43 cm were obtained on completion of 12 weeks culture period. In vitro rooting of shoots was best achieved in half-strength WPM containing 0.5 μM IBA. Well-rooted plantlets were successfully hardened off and acclimatized in plastic cups containing sterile Soilrite. These plantlets were then transferred to pots containing normal garden soil followed by transfer to greenhouse and ultimately to field under full sun.

Keywords

Micropropagation Salix tetrasperma Roxb. Shoot induction Shoot multiplication In vitro rooting 

Abbreviations

BA

6-Benzylaminopurine

NAA

α Naphthalene acetic acid

WPM

Woody plant medium

PGRs

Plant growth regulators

Notes

Acknowledgments

Financial assistance to Md Imran Khan in the form of JRF (NET) by Council of Scientific and Industrial Research (CSIR), Govt. of India, New Delhi is gratefully acknowledged. Research support provided by the Department of Science and Technology (DST) and University Grants Commission (UGC) Govt. of India, New Delhi, in the form of DST-FIST (2005) and UGC-SAP (2009) Programmes is duly acknowledged.

Supplementary material

468_2010_534_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 24 kb)

References

  1. Agrawal DC, Gebhardt K (1994) Rapid micropropagation of hybrid willow (Salix) established by ovary culture. J Plant Physiol 143:763–765Google Scholar
  2. Ahmad N, Anis M (2007) Rapid clonal multiplication of a woody tree, Vitex negundo L. through axillary shoots proliferation. Agroforest Syst 71:195–200CrossRefGoogle Scholar
  3. Ahmad N, Anis M (2010) An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L. Eur J For Res. doi:  10.1007/s10342-010-0415-y
  4. Ahmad N, Wali SA, Anis M (2008) In vitro production of true-to-type plants of Vitex negundo L. from nodal explants. J Hortic Sci Biotech 83(3):313–317Google Scholar
  5. Ahuja MR (1993) Regeneration and germplasm in aspen—Populus. In: Ahuja MR (ed) Micropropagation of woody plants. Kluwer, Dordrecht, pp 187–194Google Scholar
  6. Amo Marco JB, Lledo MD (1996) In vitro propagation of Salix tarraconensis Pau ex Font Quer, an endemic and threatened plant. In Vitro Cell Dev Biol Plant 32:42–46CrossRefGoogle Scholar
  7. Anis M, Husain MK, Faisal M, Shahzad A, Ahmad N, Siddique I, Khan H (2009) In vitro approaches for plant regeneration and conservation of some medicinal plants. In: Kumar A, Sopory SK (eds) Recent advances in plant biotechnology and its applications, vol 14. IK International Pvt Ltd, New Delhi, pp 397–410Google Scholar
  8. Anis M, Varshney A, Siddique I (2010) In vitro clonal propagation of Balanites aegyptiaca (L.) Del. Agroforest Syst 78:151–158CrossRefGoogle Scholar
  9. Anonymous (2003) The wealth of India: a dictionary of Indian raw materials and industrial products, CSIR, New Delhi, 169–175Google Scholar
  10. Bergman L, von Arnold S, Erickson T (1985) Effects of N6-benzyladenine on shoots of five willow clones (Salix spp) cultured in vitro. Plant Cell Tissue Organ Cult 4:135–144CrossRefGoogle Scholar
  11. Bhojwani SS (1980) Micropropagation method for a hybrid willow (Salix matsudana X alba NZ-1002). N Z J Bot 18:209–214Google Scholar
  12. Chalupa V (1983) Micropropagation of conifer and broad leaved forest trees. Commun Instit Forest Cechoslov 13:7–39Google Scholar
  13. Dhir KK, Angrish R, Bajaj M (1984) Micropropagation of Salix babylonica through in vitro shoot proliferation. Proc Indian Acad Sci (Plant Sci) 93(6):655–660Google Scholar
  14. Faisal M, Anis M (2002) Rapid in vitro propagation of Rauvolfia tetraphylla L.—an endangered medicinal plant. Physiol Mol Biol Plants 8:295–299Google Scholar
  15. Faisal M, Ahmad N, Anis M (2007) An efficient micropropagation system for Tylophora indica: an endangered, medicinally important plant. Plant Biotechnol Rep 1:765–772CrossRefGoogle Scholar
  16. Gebhardt K (1992) Grudlagen der Zuhtung pharmazeutisch wertvoller Weiden. Die Holzzucht 46:9–14Google Scholar
  17. Giri CC, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees 18:115–135Google Scholar
  18. Granel T, Robinson B, Mills T, clothier B, Green S, Fung L (2002) Cadmium accumulation by willow clones used for soil conservation, stock fodder, and phytoremediation. Aust J Soil Res 40:1331–1337CrossRefGoogle Scholar
  19. Grönroos L, von Arnold S, Erikson T (1989) Callus production and somatic embryogenesis from floral explants of basket willow (Salix viminalis L.). J Plant Physiol 134:558–566Google Scholar
  20. Husain MK, Anis M (2009) Rapid in vitro multiplication of Melia azaderach L. (A multipurpose woody tree). Acta Physiol Plant 31:765–772CrossRefGoogle Scholar
  21. Johansen DA (1940) Plant microtechnique. McGraw-Hill, New York, pp 126–154Google Scholar
  22. Khalafalla MM, Hattori K (2000) Ethylene inhibitors enhance in vitro root formation on faba bean shoots regenerated on medium containing thidiazuron. Plant Growth Regul 32:59–63CrossRefGoogle Scholar
  23. Kuzovkina YA, Quigley MF (2005) Willows beyond wetlands: uses of Salix species for environmental projects. Water Air Soil Pollut 162:183–204CrossRefGoogle Scholar
  24. Lloyd G, Mc Cown B (1981) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Proc Int Plant Propag Soc 30:421–427Google Scholar
  25. Lyyra S, Lima A, Merkle SA (2006) In vitro regeneration of Salix nigra from adventitious shoots. Tree Physiol 26:969–975PubMedGoogle Scholar
  26. Neuner H, Beiderback R (1993) In vitro propagation of Salix caprea L by single node explants. Silvae Genet 42:308–310Google Scholar
  27. Park SY, Kim YW, Moon HK, Murthy HN, Choi YH, Cho HM (2008) Micropropagation of Salix pseudolasiogne from nodal explants. Plant Cell Tissue Organ Cult 93:341–346CrossRefGoogle Scholar
  28. Perttu KL, Kowalik PJ (1997) Salix vegetation filters for purification of waters and soils. Biomass Bioenergy 12:9–19CrossRefGoogle Scholar
  29. Raskin I (1992) Role of salicylic acid in plants. Annu Rev Plant Physiol Mol Biol 43:439–463CrossRefGoogle Scholar
  30. Read PE, Garton S, Tormala T (1989) Willows (Salix spp.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Trees II, vol 4. Springer, Berlin, pp 370–386Google Scholar
  31. Shukla S, Shukla SK, Mishra SK (2009) In vitro plant regeneration from seedling explants of Stereospermum personatum DC: a medicinal tree. Trees 23:409–413CrossRefGoogle Scholar
  32. Siddique I, Anis M (2009) Direct plant regeneration from nodal explants of Balanites aegyptiaca L. (Del.)—a valuable medicinal tree. New Forests 37:53–62CrossRefGoogle Scholar
  33. Stoehr MU, Cai M, Zsuffa L (1989) In vitro plant regeneration via callus culture of mature Salix exigua. Can J For Res 19:1634–1638CrossRefGoogle Scholar
  34. Thorpe TA, Harry IS, Kumar PP (1991) Application of micropropagation to forestry. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer, Dordrecht, pp 311–336Google Scholar
  35. Vervaeke P, Luyssaert S, Mertens J, Meers E, Tack FMG, Lust N (2003) Phytoremediation prospects of willow stands on contaminated sediment: a field trial. Environ Pollut 126:275–282PubMedCrossRefGoogle Scholar
  36. Vinocur B, Carmi T, Altman A, Ziv M (2000) Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media. Plant Cell Rep 19:1146–1154CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Plant Production, College of Food and Agriculture ScienceKing Saud UniversityRiyadhSaudi Arabia

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