Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)
- 145 Downloads
Rudgea jasminoides (Rubiaceae) is a tropical tree species native of the Atlantic Forest in the south of Brazil. Previous studies with leaf cell walls of R. jasminoides showed a different proportion of cross-linked glycans compared to what is usually reported for eudicots. However, due to the difficulties of working with whole plant organs, cell suspensions of R. jasminoides, consisting of predominantly undifferentiated cells with mainly primary cell walls, were used to examine cell walls and extracellular soluble polysaccharides (EP) released into the culture medium. Sugar composition and linkage analysis showed homogalacturonans, xylogalacturonans and arabinogalactans to be the predominant EP. In the cell wall, homogalacturonans and arabinogalactans are the major pectins, and xyloglucans and xylans are the major cross-linking glycans. The presence of xylogalacturonans in the R. jasminoides cell cultures seems to be related to the occurrence of a homogeneous cell suspension with loosely attached cells. Although all alkali extractions from the cell walls yielded amounts of xyloglucan that exceed those of the xylans, the latter was found in a proportion that is higher than what has been usually reported for primary cell walls of most eudicots. The xyloglucan from cell walls of cell suspension cultures of R. jasminoides has low fucosylation levels and high proportion of galactosyl residues, a branching pattern commonly found in storage cell-wall xyloglucans.
KeywordsAtlantic Forest Cell wall polysaccharides Rubiaceae Xylogalacturonans Arabinoxylan Xyloglucan
This work was financially supported by the Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP, 98/05124-8 and 05/04139-7). Thanks are also due to Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq for researcher fellowships to M.S. Buckeridge and M.R. Braga and to FAPESP for a PhD fellowship to C.J.F. Oliveira Júnior. The authors thank Dr Kelly Simões for help with the figures.
- Braga MR, Pessoni RAB, Dietrich SMC (1998) Cell wall polysaccharide composition of leaves of tropical Rubiaceae differing in phytoalexin response. Rev Bras Fisiol Veg 10:71–78Google Scholar
- Carpita NC, McCann MC (1996) Some new methods to study plant polyuronic acids and their esters. In: Townsend R, Hotchkiss A (eds) Progress in glycobiology. Marcell Dekker, New York, pp 595–611Google Scholar
- Carpita NC, Shea EM (1989) Linkage structure by gas chromatography-mass spectrometry of partially-methylated alditol acetates. In: Biermann CJ, McGinnis GD (eds) Analysis of carbohydrates by GLC and MS. CRC Press, Boca Raton, pp 155–216Google Scholar
- Fry SC, York WS, Albersheim P, Darvill A, Hayashi T, Joseleau J-P, Kato Y, Lorences EP, Maclachlan GA, McNeil M, Mort AJ, Reid JS, Seitz HU, Selvendran RR, Voragen AGJ, White AR (1993) An unambiguous nomenclature for xyloglucan-derived oligosaccharides. Physiol Plant 89:1–3. doi: 10.1111/j.1399-3054.1993.tb01778 CrossRefGoogle Scholar
- Gibeaut DM, Carpita NC (1991) Tracing the biosynthesis of the cell wall in intact cells and plants. Selective turnover and alteration of cytoplasmic and cell wall polysaccharides of millet cells in liquid culture and Zea mays seedlings. Plant Physiol 97:551–561. doi: 10.1104/pp.97.2.551 CrossRefPubMedGoogle Scholar
- McCann MC, Roberts K (1991) Architecture of the primary cell wall. In: Lloyd CW (ed) The cytoskeletal basis of plant growth and form. Academic Press, London, pp 109–129Google Scholar
- Moraes F, Hahn MG, Braga MR (2001) Comparative analysis of leaf cell-wall polysaccharides of Dialypetalanthus fuscescens and Bathysa meridionalis: evidence of biochemical similarities between Dialypetalanthaceae and Rubiaceae-Cinchonoideae. Rev Bras Bot 24:289–294. doi: 10.1590/S0100-84042001000300007 CrossRefGoogle Scholar
- Oliveira Júnior CJF, Braga MR, Buckeridge MS (2006) Efeitos de oligossacarídeos de xiloglucano sobre o metabolismo extracelular de Rudgea jasminoides durante o crescimento. Hoehnea 33:239–246Google Scholar
- Silva CA, Alves ES, Braga MR (2004) Aspectos morfo-anatômicos e citoquímicos de folhas de Alibertia myrcifolia (Spruce ex. Schm.) Schum. e Rudgea jasminoides (Cham.) Mull.Arg. (Rubiaceae). Hoehnea 31:215–223Google Scholar
- Willats WGT, McCartney L, Steele-King CG, Marcus SE, Mort AM, Huisman M, van Alebeek G-J, Schols HA, Voragen AGJ, Le Goff A, Bonnin E, Thibault J-F, Knox JP (2004) A xylogalacturonan epitope is specifically associated with plant cell detachment. Planta 218:673–681. doi: 10.1007/s00425-003-1147-8 CrossRefPubMedGoogle Scholar
- Xavier ES, Silva CA, Braga MR (2004) Eliciadores de fitoalexinas de paredes celulares de Alibertia myrcifolia e Rudgea jasminoides obtidos por autoclavagem. Hoehnea 31:23–31Google Scholar