Trees

, Volume 24, Issue 4, pp 713–722

Cell wall polysaccharides from cell suspension cultures of the Atlantic Forest tree Rudgea jasminoides (Rubiaceae)

  • Clóvis J. F. Oliveira Júnior
  • Aline A. Cavalari
  • Nicholas C. Carpita
  • Marcos S. Buckeridge
  • Marcia R. Braga
Original Paper

DOI: 10.1007/s00468-010-0441-x

Cite this article as:
Oliveira Júnior, C.J.F., Cavalari, A.A., Carpita, N.C. et al. Trees (2010) 24: 713. doi:10.1007/s00468-010-0441-x
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Abstract

Rudgea jasminoides (Rubiaceae) is a tropical tree species native of the Atlantic Forest in the south of Brazil. Previous studies with leaf cell walls of R. jasminoides showed a different proportion of cross-linked glycans compared to what is usually reported for eudicots. However, due to the difficulties of working with whole plant organs, cell suspensions of R. jasminoides, consisting of predominantly undifferentiated cells with mainly primary cell walls, were used to examine cell walls and extracellular soluble polysaccharides (EP) released into the culture medium. Sugar composition and linkage analysis showed homogalacturonans, xylogalacturonans and arabinogalactans to be the predominant EP. In the cell wall, homogalacturonans and arabinogalactans are the major pectins, and xyloglucans and xylans are the major cross-linking glycans. The presence of xylogalacturonans in the R. jasminoides cell cultures seems to be related to the occurrence of a homogeneous cell suspension with loosely attached cells. Although all alkali extractions from the cell walls yielded amounts of xyloglucan that exceed those of the xylans, the latter was found in a proportion that is higher than what has been usually reported for primary cell walls of most eudicots. The xyloglucan from cell walls of cell suspension cultures of R. jasminoides has low fucosylation levels and high proportion of galactosyl residues, a branching pattern commonly found in storage cell-wall xyloglucans.

Keywords

Atlantic Forest Cell wall polysaccharides Rubiaceae Xylogalacturonans Arabinoxylan Xyloglucan 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Clóvis J. F. Oliveira Júnior
    • 1
  • Aline A. Cavalari
    • 2
  • Nicholas C. Carpita
    • 3
  • Marcos S. Buckeridge
    • 4
  • Marcia R. Braga
    • 5
  1. 1.Núcleo de Pesquisa em Plantas OrnamentaisInstituto de BotânicaSão PauloBrazil
  2. 2.Universidade Federal de São PauloDiademaBrazil
  3. 3.Department of Plant PathologyPurdue UniversityWest LafayetteUSA
  4. 4.Departamento de BotânicaUniversidade de São PauloSão PauloBrazil
  5. 5.Núcleo de Pesquisa em Fisiologia e BioquímicaInstituto de BotânicaSão PauloBrazil

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