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, Volume 23, Issue 3, pp 539–553 | Cite as

Ozone affects shikimate pathway genes and secondary metabolites in saplings of European beech (Fagus sylvatica L.) grown under greenhouse conditions

  • Gunter Andreas Betz
  • Elke Gerstner
  • Susanne Stich
  • Barbro Winkler
  • Gerhard Welzl
  • Elisabeth Kremmer
  • Christian Langebartels
  • Werner Heller
  • Heinrich Sandermann
  • Dieter ErnstEmail author
Original Paper

Abstract

The shikimate pathway plays a central role in the formation of aromatic intermediates in the production of stilbenes, flavonoids and lignins. Ozone effects on the levels of transcripts in this pathway were studied in saplings of European beech. Complementary DNA (cDNA) clones of all genes of this pathway were isolated, and quantitative real-time RT-PCR (qRT-PCR) using RNA isolated from leaves of ozone-treated saplings showed a strong induction of 3-deoxy-d -arabino-heptulosonate-7-phosphate synthase 1 (DAHPS1), DAHPS3, 3-dehydroquinate dehydratase/shikimate dehydrogenase (DHQD/SD), 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), and chorismate mutase (CM) transcripts. In contrast, DAHPS2, 3-dehydroquinate synthase (DHQS), shikimate kinase (SK), and chorismate synthase (CS) transcripts were only weakly induced. Earliest induction could be observed after 2 days of ozone treatment for DAHPS1, SK, EPSPS and CM. The coordinated regulation was evident for 3–5 weeks after the onset of ozone fumigation, and increased transcript levels were still detectable after another 7 weeks. Western blot analyses of DAHPS3 and DHQD/SD showed an increased protein level in agreement with the increased transcription levels. Ozone-dependent leaf lesions appeared 7 weeks after onset of ozone exposure. Strongly elevated were levels of conjugates of salicylic (SA) and gentisic acids (GA), either derived directly from chorismate, the key product of the shikimate pathway, or via phenylalanine, cinnamic, and benzoic acids. Concentrations of cell wall-bound phenolic compounds increased in both control and ozone-treated saplings with the latter showing slightly higher levels. Interestingly, however, this increase of cell wall-bound phenolics was accompanied by a decrease of soluble phenolics, which may indicate their deposition into the cell wall.

Keywords

Fagus sylvatica Gene expression Ozone Phenolic metabolites Shikimate pathway 

Notes

Acknowledgments

We wish to thank the technical staff of EUS for their excellent assistance. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 607) and in part by the European Community (Evoltree, 6th Framework Program; COST E52).

Supplementary material

468_2008_300_MOESM1_ESM.doc (66 kb)
Supplementary material (DOC 66 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Gunter Andreas Betz
    • 1
    • 5
  • Elke Gerstner
    • 1
  • Susanne Stich
    • 1
  • Barbro Winkler
    • 2
  • Gerhard Welzl
    • 3
  • Elisabeth Kremmer
    • 4
  • Christian Langebartels
    • 1
  • Werner Heller
    • 1
  • Heinrich Sandermann
    • 1
    • 6
  • Dieter Ernst
    • 1
    Email author
  1. 1.Institute of Biochemical Plant PathologyHelmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  2. 2.Department of Environmental EngineeringHelmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  3. 3.Institute of Developmental GeneticsHelmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  4. 4.Institute of Molecular ImmunologyHelmholtz Zentrum München, German Research Center for Environmental HealthMunichGermany
  5. 5.Olympus Life Science Research Europa GmbHMunichGermany
  6. 6.FreiburgGermany

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