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Trees

, Volume 23, Issue 2, pp 335–338 | Cite as

Plant regeneration through callus cultures derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.)

  • Omid Karami
  • Khosro PiriEmail author
  • Reza Bahmani
Original Paper

Abstract

Efficient plant regeneration was achieved from callus derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.). Calli were obtained on MS media containing 3% sucrose and different concentrations of TDZ. The highest rate of green, compact and nodular callus was formed on MS medium supplemented with 1 mg/l of TDZ. Shoot organogenesis was achieved when the callus was transferred onto MS media containing 3% sucrose and BA alone (05–4 mg/l) or BA (0.5 and 1 mg/l) combined with NAA or IAA (0.5 and 1 mg/l). Maximum organogenesis was obtained with 1 mg/l BA in combination with 0.5 mg/l NAA. Rooting of the shoots was achieved on MS medium supplemented with 0.2 mg/l IBA. Regenerated plantlets were acclimatized and successfully transplanted to soil.

Keywords

Oleaster Plant regeneration Callus 

Abbreviations

BA

6-Benzyladenine

2,4-D

2,4-Dichlorophenoxyacetic acid

MS

Murashige and Skoog (1962) basal medium

NAA

α-Naphthalene acetic acid

IBA

Indole-3-butyric acid

TDZ

N-phenyl-N-1,2,3-thidiazol-5-yiurea

IAA

Indole-3-acetic acid

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of AgricultureBu-Ali Sina UniversityHamadanIran
  2. 2.Department of Horticulture, Faculty of AgricultureBu-Ali Sina UniversityHamadanIran

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