, Volume 23, Issue 2, pp 335–338 | Cite as

Plant regeneration through callus cultures derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.)

  • Omid Karami
  • Khosro PiriEmail author
  • Reza Bahmani
Original Paper


Efficient plant regeneration was achieved from callus derived from immature-cotyledon explants of oleaster (Elaeagnus angustifolia L.). Calli were obtained on MS media containing 3% sucrose and different concentrations of TDZ. The highest rate of green, compact and nodular callus was formed on MS medium supplemented with 1 mg/l of TDZ. Shoot organogenesis was achieved when the callus was transferred onto MS media containing 3% sucrose and BA alone (05–4 mg/l) or BA (0.5 and 1 mg/l) combined with NAA or IAA (0.5 and 1 mg/l). Maximum organogenesis was obtained with 1 mg/l BA in combination with 0.5 mg/l NAA. Rooting of the shoots was achieved on MS medium supplemented with 0.2 mg/l IBA. Regenerated plantlets were acclimatized and successfully transplanted to soil.


Oleaster Plant regeneration Callus 





2,4-Dichlorophenoxyacetic acid


Murashige and Skoog (1962) basal medium


α-Naphthalene acetic acid


Indole-3-butyric acid




Indole-3-acetic acid


  1. Economou AS, Maloupa EM (1995) Regeneration of Elaeagnus angustifolia from leaf segments of in vitro derived shoots. Plant Cell Tissue Organ Cult 40:285–288. doi: 10.1007/BF00048135 CrossRefGoogle Scholar
  2. Economou AS, Spanoudaki MJ (1988) Regeneration in vitro of oleaster (Elaeagnus angustifolia L.) from shoot tips of mature tree. Acta Hortic 227:363–368Google Scholar
  3. Hosseinzadeh H, Ramezani M, Namjo N (2003) Muscle relaxant activity of Elaeagnus angustifolia L. fruit seeds in mice. J Ethnopharmacol 84:275–278. doi: 10.1016/S0378-8741(02)00331-8 PubMedCrossRefGoogle Scholar
  4. Iriondo M, Delaiglesia M, Perez A (1995) Micropropagation of Elaeagnus angustifolia from mature trees. Tree Physiol 15:691–693PubMedGoogle Scholar
  5. Klich MG (2000) Leaf variations in Elaeagnus angustifolia related to environment heterogeneity. Environ Exp Bot 44:171–183. doi: 10.1016/S0098-8472(00)00056-3 PubMedCrossRefGoogle Scholar
  6. Koroch A, Juliani HR, Kapteyn J, Simon JE (2002) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tiss Org Cult 69:79–83. doi: 10.1023/A:1015042032091 CrossRefGoogle Scholar
  7. Li Y, Ma L, Ozaki H, Hisajima S (2004) Micropropagation of Elaeagnus angustifolia by rebroductive organ culture in vitro. Jpn J Trop Agric 48(1):23–24Google Scholar
  8. Lu CY (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol 29:92–96CrossRefGoogle Scholar
  9. Lucchesini M, Mensuali Sodi A (1996) Regeneration of oleaster (Elaeagnus angustifolia L.) was achieved from different types of explants. Gartenbauwissenschaft 61:276–280Google Scholar
  10. Mirhydar H (1998) Encyclopedia of plants: indications of plants in the prevention and treatment of disease,vol 2. Islamic Farhang, Tehran, pp 163–164Google Scholar
  11. Murashige T, Skoog FA (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 154:73–479Google Scholar
  12. Murthy BNS, Murch SJ, Saxena PK (1995) Thidiazuron induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea L.): endogenous growth regulator levels and significance of cotyledons. Physiol Plant 94:268–276. doi: 10.1111/j.1399-3054.1995.tb05311.x CrossRefGoogle Scholar
  13. Pereira AM, Bertoni BW, Appezzato-da-Gloria B, Araujo ARB, Januario AH, Lourenco MV et al (2000) Micropropagation of Pothomorphe umbellate via direct organogenesis from leaf explants. Plant Cell Tiss Org Cult 60:47–53. doi: 10.1023/A:1006409807719 CrossRefGoogle Scholar
  14. Perry LM (1980) Medicinal plants of the East and Southeast Asia. MIT Press, London, p 131Google Scholar
  15. Victor JMR, Murch SJ, Krishnaraj S, Saxena PK (1999) Somatic embryogenesis and organogenesis in peanut: the role of thidiazuron and N6-benzylaminopurine in the induction of plant morphogenesis. Plant Growth Regul 28:9–15. doi: 10.1023/A:1006274615736 CrossRefGoogle Scholar
  16. Visser C, Fletcher RA, Saxena PK (1995) Thidiazuron stimulates expansion and greening in cucumber cotyledons. Physiol Mol Biol Plants 1:21–25Google Scholar
  17. Xie DY, Hong Y (2001) Regeneration of Acacia mangium through somatic embryogenesis. Plant Cell Rep 20:34–40. doi: 10.1007/s002990000288 CrossRefGoogle Scholar
  18. Zargari A (1990) Medicinal plants, vol 4. Tehran University Press, Tehran, pp 275–277Google Scholar
  19. Zhang BZ, Zhao KF (1996) Study on salt tolerance in Robinia and Elaeagnus angustifolia. Shandong Sci 9:53–55Google Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Biotechnology, Faculty of AgricultureBu-Ali Sina UniversityHamadanIran
  2. 2.Department of Horticulture, Faculty of AgricultureBu-Ali Sina UniversityHamadanIran

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