Trees

, 22:779 | Cite as

DNA methylation in different origin clonal offspring from a mature Sequoiadendron giganteum genotype

  • Olivier Monteuuis
  • Sylvie Doulbeau
  • Jean-Luc Verdeil
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

A meristem-issued rejuvenated line was obtained in 1986 from a 100-year-old Sequoiadendron giganteum tree and has been since then micropropagated in tissue culture conditions maintaining its juvenile-like characteristics. By contrast, grafts and rooted microcuttings from the same genotype planted in outdoor conditions for several years exhibited mature foliage traits and the grafts started to produce cones, which are obvious indicators of physiological aging. These three different clonal lines were compared with regard to global DNA methylation assessed by HPLC. The in vitro rejuvenated line showed a much higher level of DNA methylation (23% as average value) than the two other outdoor origins from the same clone which displayed similar degrees of global methylation (average values of 13.4% for the grafts and 13.8% for the cuttings). Overall these DNA global methylation values obtained for the first time in S. giganteum are consistent with the level of methylation reported for many plants using the same HPLC protocols. The fact that shoots exhibiting a juvenile-like leaf morphology can be characterized by higher DNA methylation than mature-like ones is discussed in relation to physiological aging, referring to other studies on the same topic.

Keywords

Aging Coniferous species DNA methylation Maturational traits Phase change 
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Notes

Acknowledgments

We are deeply indebted to Mrs. Elisabeth Dumas from AFOCEL who had been subculturing the “R86” rejuvenated line in AFOCEL tissue culture facilities for several years.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Olivier Monteuuis
    • 1
  • Sylvie Doulbeau
    • 2
  • Jean-Luc Verdeil
    • 3
  1. 1.CIRAD-BIOS, UPR 39, Campus international de Baillarguet TA 10/CMontpellier Cedex 5France
  2. 2.IRD, UMR DIAPCMontpellier Cedex 5France
  3. 3.CIRAD-BIOS, UMR DAP PHIVMontpelier Cedex 5France

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