, 22:531 | Cite as

Gas chromatographic–mass spectrometric investigation of metabolites from the needles and roots of pine seedlings at early stages of pathogenic fungi Armillaria ostoyae attack

  • Valery A. IsidorovEmail author
  • Paweł Lech
  • Anna Żółciak
  • Magdalena Rusak
  • Lech Szczepaniak
Original Paper


An investigation was carried out of the composition of metabolites in pine seedlings tissues at the initial stages of the infectious process caused by pathogenic fungi Armillaria ostoyae, which causes a root rot of trees and degradation of forest resources. With the help of successive extraction with organic solvents of different polarity, more than 190 metabolites were extracted from the needles and roots of the seedlings and then identified by GC–MS method. The composition of the extracts from control plants and those inoculated with Armillaria ostoyae were compared. It was established that part of secondary metabolites (glucosamines and free amino acids, carbohydrates raffinose and trehalose) were present only in the tissues of inoculated plants. Possible roles of some of these compounds appearing in the roots of seedlings infected with the fungus are also discussed in the paper.


Armillaria root disease Scots pine seedlings Metabolites composition GC–MS analysis 



This work was supported by Committee for Scientific Investigation (KBN) of Poland, grant N 6 PO6L 028 21.


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Valery A. Isidorov
    • 1
    Email author
  • Paweł Lech
    • 2
  • Anna Żółciak
    • 2
  • Magdalena Rusak
    • 1
  • Lech Szczepaniak
    • 1
  1. 1.Institute of ChemistryBiałystok UniversityBiałystokPoland
  2. 2.Department of Forest PhytopathologyForest Research InstituteRaszynPoland

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