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Pediatric Nephrology

, Volume 31, Issue 9, pp 1469–1476 | Cite as

Urine heat shock protein 70 levels as a marker of urinary tract infection in children

  • Alev Yilmaz
  • Zeynep Yuruk YildirimEmail author
  • Sevinc Emre
  • Asuman Gedikbasi
  • Tarik Yildirim
  • Ahmet Dirican
  • Evren Onay Ucar
Original Article

Abstract

Background

Heat shock proteins (HSPs) are a multi-family group of proteins which are upregulated by the cell in response to exposure to hazardous (stress) factors, including infectious agents, to prevent changes in protein structure. The aim of our study was to assess whether urine levels of the 70-kDa family of HSPs (HSP70s) increase in children with urinary tract infection (UTI) and to determine the optimal urine (u) HSP70 cut-off level to predict UTI in children.

Methods

Forty patients with symptomatic UTI (UTI group), 30 healthy children (control group), 21 asymptomatic patients with proven bacterial contamination in their urine culture (contamination group) and 30 patients with fever caused by other infections (non-UTI infection group) were enrolled in the study. Random urine samples were obtained for measurement of HSP70 and creatinine (Cr) from all groups. Urine was collected prior to the treatment of UTI at the time of presentation and after treatment. Urine HSP70 levels were measured by enzyme-linked immunosorbent analysis. A dimercaptosuccinic acid (DMSA) scan was performed at 5–7 days after presentation in UTI group to distinguish patients with acute pyelonephritis from those with cystitis; based on this scan, no patients had acute pyelonephritis. Patients were classified with pyelonephritis in the presence of all of the following signs: axillary fever of ≥39 °C, leukocytosis and positivity for C-reactive protein.

Results

The mean urine HSP70:Cr ratio (uHSP70/Cr) prior to treatment was significantly higher in the UTI group (449.86 ± 194.33 pg/mg) than in the control, contamination and non-UTI infection groups (39.93 ± 47.61, 32.43 ± 9.09 and 45.14 ± 19.76, respectively; p = 0.0001). Using a cut-off of 158 pg/mg uHSP70/Cr for the prediction of UTI, the sensitivity and specificity of the assay were 100 and 100 %, respectively (area under the time–concentration curve = 1). The uHSP70/Cr was highest in the patients with clinical pyelonephritis (p = 0.001). Mean uHSP70/Cr after treatment decreased to 60.68 ± 51.11 pg/mg in UTI group (p = 0 .0001).

Conclusions

Our findings suggest that elevated uHSP70/Cr may be a useful biomarker for the prediction of UTI in children, with a high sensitivity and specificity, and that they may help to distinguish UTI from other infections as well as bacterial contamination of the urine.

Keywords

Urine heat shock proteins Urinary tract infection Children Biomarker HSP 

Notes

Compliance with ethical standards

This study was approved by the local ethics committee (2012/887-1083). Informed consent was obtained from parents of all children participating in the study.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© IPNA 2016

Authors and Affiliations

  • Alev Yilmaz
    • 1
  • Zeynep Yuruk Yildirim
    • 1
    Email author
  • Sevinc Emre
    • 1
  • Asuman Gedikbasi
    • 2
  • Tarik Yildirim
    • 3
  • Ahmet Dirican
    • 4
  • Evren Onay Ucar
    • 5
  1. 1.Istanbul Faculty of Medicine, Pediatric Nephrology DepartmentIstanbul UniversityCapaTurkey
  2. 2.Biochemistry DepartmentBakirkoy Dr. Sadi Konuk Training and Research HospitalIstanbulTurkey
  3. 3.Pediatrics DepartmentKanuni Sultan Süleyman Training and Research HospitalIstanbulTurkey
  4. 4.Biostatistics Department, Istanbul Faculty of MedicineIstanbul UniversityIstanbulTurkey
  5. 5.Department of Molecular Biology & Genetics, Faculty of ScienceIstanbul UniversityIstanbulTurkey

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