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Pediatric Nephrology

, Volume 29, Issue 12, pp 2325–2331 | Cite as

Prevalence of herpesviruses at onset of idiopathic nephrotic syndrome

  • Claire Dossier
  • Anne-Laure Sellier-Leclerc
  • Alexandra Rousseau
  • Yanne Michel
  • Agnès Gautheret-Dejean
  • Mariana Englender
  • Fouad Madhi
  • Marina Charbit
  • Tim Ulinski
  • Tabassome Simon
  • Evelyne Jacqz-Aigrain
  • Georges DeschênesEmail author
Original Article

Abstract

Background

Idiopathic nephrotic syndrome (INS) is likely a primary immune disorder, but viruses might also be involved in the mechanisms of the disease. Here, we investigate the link between herpesvirus infection and the first manifestation of INS in children.

Methods

A prospective, multicentre, and population-based case–control study called NEPHROVIR included 164 patients, aged 6 months to 15 years old, newly diagnosed with INS, and 233 controls matched for gender, age, and period of sample. The analysis was done on 124 patients and 196 controls. Epstein–Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus-6 (HHV-6), and human herpesvirus-7 (HHV-7) DNA prevalence at diagnosis were assessed from whole peripheral blood samples, as well as EBV and CMV viral load and seroprevalence.

Results

EBV DNA was significantly more prevalent in cases than in controls (50.8 vs 29.1 %; OR = 2.6; p = 0.0002), with no difference in viral load. A significant difference was also found for CMV (11.3 vs 3.6 %; p = 0.02) and HHV-7 (83 vs 72 %; p = 0.02) DNA prevalence between cases and controls. There were significantly more EBV and CMV recent infections or reactivations based on VCA-IgM and CMV IgM in cases than controls, while there were no differences in IgG seroprevalence.

Conclusion

The prevalence of positive EBV DNA detection and recent infection or reactivation is higher in children at onset of INS compared to a population matched for age, gender, and time of sampling.

Keywords

Epstein-barr virus Cytomegalovirus Steroid-sensitive nephrotic syndrome Proteinuria Nephrovir 

Notes

Acknowledgments

We acknowledge paediatricians from participating centers for their help and contribution to this study: Dr. Orzechowski (Department of pediatrics, Centre Hospitalier Sainte Camille, Bry sur Marne); Dr. Nathanson (Department of pediatrics, Centre Hospitalier de Versailles); Dr. May (Department of pediatrics, Centre Hospitalier d’Evry); Dr. Zemouri & Dr. Ferré (Department of pediatrics, Centre Hospitalier Intercommunal de Montreuil); Pr. Chevallier (Department of pediatrics, Ambroise-Paré Hospital, APHP); Dr. Lelorier (Department of pediatrics, Centre Hospitalier de Melun); Dr. Gire (Department of pediatrics, Centre Hospitalier de Villeneuve Saint Georges); Dr. Elias (Department of pediatrics, Centre Hospitalier de Gonnesse); Dr. Questiaux (Department of pediatrics, Centre Hospitalier d’Aulnay); Dr. Mesples (Department of pediatrics, Louis Mourier Hospital); Dr. Gouraud & Dr. Larakeb (Department of pediatrics, Centre Hospitalier de Meaux); Dr. Furioli (Department of pediatrics, Centre Hospitalier de Mantes la Jolie); Dr. Talon (Department of pediatrics, Centre Hospitalier de Montfermeil); Dr. Seaume (Department of pediatrics, Centre Hospitalier de Longjumeau); Dr. Blanc (Department of pediatrics, Centre Hospitalier de Poissy-Saint Germain); Dr. Colin-Gorski (Department of pediatrics, Centre Hospitalier d’Argenteuil); Dr. de Pontual (Department of pediatrics, Jean Verdier Hospital); Dr. Douillet (Department of pediatrics, Centre Hospitalier de Fontainebleau); Pr. Kone Paut (Department of pediatrics, Bicetre Hospital, APHP); Dr. de Gennes (Department of pediatrics, Centre Hospitalier d’Orsay); Dr. Muller (Department of pediatrics, Centre Hospitalier de Rambouillet); Dr. Amira (Department of pediatrics, Centre Hospitalier de Saint Denis); Dr. Gajdos (Department of pediatrics, Antoine-Beclère Hospital, APHP); Dr. Akil (Department of pediatrics, Centre Hospitalier de Neuilly); Dr. Monier (Department of pediatrics, Centre Hospitalier de Montmorency); Dr. Blimpo (Department of pediatrics, Centre Hospitalier des Portes de l’Oise); Dr. Copin (Department of pediatrics, Centre Hospitalier de Provins); Dr. Branca (Department of pediatrics, Centre Hospitalier de Montereau); Dr. Tahiri (Department of pediatrics, Centre Hospitalier de Dourdan); Dr. Chalvon (Department of pediatrics, Centre Hospitalier de Lagny); Dr. Nacer (Department of pediatrics, Centre Hospitalier de Coulommiers); Dr. Saf (Department of pediatrics, Centre Hospitalier d’Arpajon); Dr. Gaagliardone (Department of pediatrics, Hopital Franco-Britannique); Pr. Gendrel (Saint Vincent de Paul hospital, APHP); Pr. Cheron (Necker Hospital, APHP).

Disclosure

All the authors declared no competing interests.

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Copyright information

© IPNA 2014

Authors and Affiliations

  • Claire Dossier
    • 1
  • Anne-Laure Sellier-Leclerc
    • 2
  • Alexandra Rousseau
    • 3
  • Yanne Michel
    • 4
  • Agnès Gautheret-Dejean
    • 5
  • Mariana Englender
    • 6
  • Fouad Madhi
    • 7
  • Marina Charbit
    • 8
  • Tim Ulinski
    • 9
  • Tabassome Simon
    • 3
  • Evelyne Jacqz-Aigrain
    • 10
  • Georges Deschênes
    • 1
    Email author
  1. 1.Service de Néphrologie PédiatriqueHôpital Robert DebréParisFrance
  2. 2.Service de Néphrologie PédiatriqueHospices Civiles de LyonLyonFrance
  3. 3.Unité de Recherche Clinique URC-EstUniversité Paris 6ParisFrance
  4. 4.Departement de Microbiologie Hôpital Armand Trousseau, APHPParisFrance
  5. 5.Laboratoire de VirologieHôpital Pitié-Salpêtrière, APHPParisFrance
  6. 6.Service de PédiatrieHôpital René DubosPontoiseFrance
  7. 7.Service de PédiatrieCentre Hospitalier IntercommunalCréteilFrance
  8. 8.Service de Néphrologie PédiatriqueNecker Enfants Malades, APHPParisFrance
  9. 9.Service de Néphrologie PédiatriqueHôpital Armand Trousseau, APHPParisFrance
  10. 10.Centre d’Investigation Clinique, APHPParisFrance

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