Pediatric Nephrology

, Volume 28, Issue 9, pp 1803–1812 | Cite as

Serum from minimal change patients in relapse increases CD80 expression in cultured podocytes

  • Takuji Ishimoto
  • Gabriel Cara-Fuentes
  • Heiman Wang
  • Michiko Shimada
  • Clive H. Wasserfall
  • William E. Winter
  • Christopher J. Rivard
  • Carlos E. Araya
  • Moin A. Saleem
  • Peter W. Mathieson
  • Richard J. Johnson
  • Eduardo H. Garin
Original Article

Abstract

Background

Minimal change disease (MCD) is the most common cause of nephrotic syndrome in children and is associated with the expression of CD80 in podocytes and the increased excretion of CD80 in urine. We hypothesized that serum from patients with MCD might stimulate CD80 expression in cultured podocytes.

Methods

Sera and peripheral blood mononuclear cells (PBMCs) were collected from subjects with MCD in relapse and remission and from normal controls. Immortalized human podocytes were incubated with culture media containing patient sera or supernatants from patient and control PBMC cultures. CD80 expression was measured by quantitative PCR and western blot analysis.

Results

Sera collected from patients with MCD in relapse, but not in remission, significantly increased CD80 expression (mean ± standard deviation: 1.8 ± 0.7 vs. 0.8 ± 0.2; p < 0.004) and CD80 protein secretion by podocytes (p < 0.05 between relapse and normal controls). No such CD80 increase was observed when podocytes were incubated with supernatants of PBMC cultures from patients in relapse.

Conclusions

Sera from MCD patients in relapse, but not in remission, stimulated CD80 expression in cultured podocytes. Identifying this factor in sera could provide insights into the pathogenesis of this disorder. No role in CD80 expression by podocytes was found for cytokines released by PBMCs.

Keywords

CD80 Minimal change disease Nephrotic syndrome 

Notes

Conflict of interest

We, the authors declare that the results presented in this paper have not been published previously in whole or part, except in abstract format. This work was supported by NIH R01DK080764. The authors declare no conflicts of interest.

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Copyright information

© IPNA 2013

Authors and Affiliations

  • Takuji Ishimoto
    • 1
  • Gabriel Cara-Fuentes
    • 2
  • Heiman Wang
    • 2
  • Michiko Shimada
    • 1
  • Clive H. Wasserfall
    • 3
  • William E. Winter
    • 3
  • Christopher J. Rivard
    • 1
  • Carlos E. Araya
    • 2
  • Moin A. Saleem
    • 4
  • Peter W. Mathieson
    • 4
  • Richard J. Johnson
    • 1
  • Eduardo H. Garin
    • 2
  1. 1.Division of Renal Diseases and Hypertension, Department of MedicineUniversity of ColoradoDenverUSA
  2. 2.Division of Pediatric Nephrology, Department of PediatricsUniversity of FloridaGainesvilleUSA
  3. 3.Department of PathologyUniversity of FloridaGainesvilleUSA
  4. 4.Academic Renal UnitUniversity of BristolBristolUK

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