RETRACTED ARTICLE: Soy protein prevents renal damage in a fructose-induced model of metabolic syndrome via inhibition of NF-kB in male rats
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The study determines the effect of soy protein on inflammatory status and expression of nuclear factor-kappa B (NF-κB P65) and receptor for advanced glycation end products (RAGE) in a metabolic syndrome (MS) model. MS was induced in adult male rats by feeding them a high fructose diet (60 g/100 g diet). The rats were randomised into six groups by feeding one of the following semi-synthetic diets for 60 days: corn starch (60%) and casein (20%; CCD), fructose (60%) and casein (20%; FCD), fructose (60%) and soy protein (20%; FSD) or corn starch (60%) and soy protein (20%; CSD). The expression of NF-κB P65, transforming growth factor-β1 (TGF-β1) and RAGE, histochemical localization of α-smooth muscle actin (α-SMA), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) assays, collagen deposition and ultrastructural analysis were performed. FCD rats displayed inflammatory changes and increased expression of growth factors and nuclear factors. FSD rats showed reduction in inflammation, fibrogenesis, collagen deposition, NF-κB activation and mitigated the ultrastructural changes. Soy protein prevents inflammation and early nephropathic changes in the MS model secondary to the attenuation of NF-κB activation.
KeywordsKidney Soy protein Fructose Insulin resistance Collagen
The financial support in the form of a Senior Research Fellowship to Mr. N. Palanisamy from the Indian Council of Medical Research (ICMR), New Delhi is gratefully acknowledged. The authors thank Mr. Manickam M, Sakthi Sugars Pvt Ltd, a division of Sakthi Soya Pvt Ltd, Coimbatore, India for providing soy protein. The authors are also thankful to Dr. Ramamurthy, Director, National Centre for Ultrafast Process, Dr. Rama Gopalan, Head, Department of Pathology, University of Madras, Chennai, India for their help in immunohistochemistry and confocal microscopy.
- 2.Sánchez-Lozada LG, Tapia E, Jiménez A, Bautista P, Cristóbal M, Nepomuceno T, Soto V, Avila-Casado C, Nakagawa T, Johnson RJ, Herrera-Acosta J, Franco M (2007) Fructose-induced metabolic syndrome is associated with glomerular hypertension and renal microvascular damage in rats. Am J Physiol Ren Physiol 292:F423–F429CrossRefGoogle Scholar
- 12.Tovar AR, Murguía F, Cruz C, Hernández-Pando R, Aguilar-Salinas CA, Pedraza-Chaverri J, Correa-Rotter R, Torres N (2002) A soy protein diet alters hepatic lipid metabolism gene expression and reduces serum lipids and renal fibrogenic cytokines in rats with chronic nephrotic syndrome. J Nutr 132:2562–2569PubMedGoogle Scholar
- 13.Lavigne C, Marette A, Jacques H (2000) Cod and soy proteins compared with casein improve glucose tolerance and insulin sensitivity in rats. Am J Physiol Endocrinol Metab 278:491–500Google Scholar