The renal adverse effects of ibuprofen are not mediated by AQP2 water channels
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The aim of this study was to determine (1) whether ibuprofen treatment in very preterm infants causes an increase in the renal water channel aquaporin-2 (AQP2) activity in the collecting duct via prostaglandin synthesis inhibition and (2) whether AQP2 activity remains disturbed long after ibuprofen treatment has ended. This was a prospective study involving premature infants with a gestation age of 27–31 weeks who received treatment between December 2005 and August 2006 in a tertiary Neonatal Intensive Care Unit. Each ibuprofen-treated infant was matched to two controls. Renal glomerular and tubular function were evaluated weekly for 1 month, and urinary AQP2 was measured by immuno-dotting. In total, 166 longitudinal samples were analyzed in 36 infants. Median [interquartile range] gestational age and birthweight were 28 [27.0–29.5] weeks and 1160 [1041–1242] g, respectively. Perinatal factors were similar in both groups. Urine output was significantly decreased in the ibuprofen-treated infants during the treatment. The urinary AQP2 level decreased significantly from day 2 to day 7 in both groups and was similar thereafter for the first month of life in ibuprofen-treated and control groups. Based on our results, we conclude that ibuprofen-induced oligo-anuria is not associated with a change in AQP2 activity and that ibuprofen does not affect AQP2 activity during the first month of life in very preterm neonates.
KeywordsAquaporin-2 Ibuprofen Neonatal Intensive Care Unit Patent ductus arteriosus Renal function
This study was supported by an unrestricted grant of the French non-benefit APRESAN Association, and the French non-benefit Association des Chefs de Service, Nancy.
Statement of financial support
This study benefited from an unrestricted grant of the French non-benefit APRESAN Association.
- 1.Bueava A, Guignard JP (1992) Renal function in preterm infants. Pediatr Res 36:572–577Google Scholar
- 22.Ecelbarger CA, Fernandez-Llama P, Lee AJ, Nielsen S, Ware JA, Shen S, Pohl LR, Knepper MA (1997) Enhancement of urinary concentrating ability by cyclooxygenase inhibitors is associated with increased expression of Na-K-2Cl cotransporter and increased trafficking of aquaporin-2 water channel (Abstract). J Am Soc Nephrol 8:413Google Scholar
- 26.Ohlsson A, Walia R, Shah S (2008) Ibuprofen for the treatment of patent ductus arteriosus in preterm and/or low birth weight infants. Cochrane Database Syst Rev (1):CD003481Google Scholar
- 31.Desfrere L, Zohar S, Morville P, Brunhes A, Chevret S, Pons G, Moriette G, Rey E, Treluyer JM (2003) Dose-finding study of Ibuprofen in patent ductus arteriosus. Biol Neonate 84:272Google Scholar
- 33.Rasband WS (1997-2009) Image J. U.S. National Institutes of Health, Bethesda. Available at: http://rsb.info.nih.gov/ij/