Pediatric Nephrology

, Volume 24, Issue 11, pp 2217–2223 | Cite as

Six-minute walking test in children with ESRD: discrimination validity and construct validity

  • Tim Takken
  • Raoul Engelbert
  • Monique van Bergen
  • Jaap Groothoff
  • Jeroen Nauta
  • Koen van Hoeck
  • Marc Lilien
  • Paul Helders
Open Access
Original Article


The six-minute walking test (6MWT) may be a practical test for the evaluation functional exercise capacity in children with end-stage renal disease (ESRD). The aim of this study was to investigate the 6MWT performance in children with ESRD compared to reference values obtained in healthy children and, secondly, to study the relationship between 6MWT performance with anthropometric variables, clinical parameters, aerobic capacity and muscle strength. Twenty patients (13 boys and seven girls; mean age 14.1 ± 3.4 years) on dialysis participated in this study. Anthropometrics were taken in a standardized manner. The 6MWT was performed in a 20-m-long track in a straight hallway. Aerobic fitness was measured using a cycle ergometer test to determine peak oxygen uptake \( \left( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \right) \), peak rate (Wpeak) and ventilatory threshold (VT). Muscle strength was measured using hand-held myometry. Children with ESRD showed a reduced 6MWT performance (83% of predicted, p < 0.0001), irrespective of the reference values used. The strongest predictors of 6MWT performance were haematocrit and height. Regression models explained 59% (haematocrit and height) to 60% (haematocrit) of the variance in 6MWT performance. 6MWT performance was not associated with \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \), strength, or other anthropometric variables, but it was significantly associated with haematocrit and height. Children with ESRD scored lower on the 6MWT than healthy children. Based on these results, the 6MWT may be a useful instrument for monitoring clinical status in children with ESRD, however it cannot substitute for other fitness tests, such as a progressive exercise test to measure \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \) or muscle strength tests.


Exercise capacity Kidney disease Muscle strength Physical activity 


Children with end-stage renal disease (ESRD) are characterized by multiple factors that influence their exercise capacity, such as anaemia, metabolic acidosis, electrolyte imbalance, osteopaenia, growth failure, undernutrition, fluid imbalance, muscle wasting and a sedentary life style [1, 2].

In recent years exercise training has attracted interest as a potential means to improve the stamina, endurance and physical activity capacity of children and adults with ESRD [3, 4, 5]. A practical test for the evaluation of the physical activity capacity or functional exercise capacity of patients with ESRD might be the six-minute walking test (6MWT) [4, 6].

The 6MWT is a self-paced, submaximal exercise test used to assess functional exercise capacity in children with chronic diseases. It has been widely used in adults, most extensively in patients with cardiopulmonary diseases, and is currently the test of choice when a functional walk test is required for clinical or research purposes [7]. The test is well-standardized and easy to administer. The 6MWT is increasingly being utilized in paediatric populations [8, 9, 10] and has been found to be a valid estimate of physical fitness in, for example, children with severe cardiopulmonary disease, cystic fibrosis (CF) and juvenile idiopathic arthritis (JIA) [11, 12, 13]. The test has shown good reliability [14, 15] and is frequently employed to assess the response to interventions [4, 16, 17]. However, there are some limitations to this test; for example, it is unclear how hard the patient is working and how its score is related to other objective measures of exercise capacity in children with chronic conditions like ESRD.

Reference values and prediction equations from healthy children have recently been published, making it possible to determine the predicted 6MWD for individual patients [18, 19, 20]. Age, height, weight and gender are known to independently predict 6MWD in healthy adults [21]. In children, varying associations have been reported between anthropometric variables, fitness variables and 6MWD [11, 13, 14, 15, 18, 19, 22].

The aim of the study reported here was to investigate 6MWT performance in children with ESRD compared to reference values obtained in healthy children (discrimination validity) and, secondly, to study the relationship between 6MWT performance with anthropometric variables, clinical variables, aerobic fitness and muscle strength (construct validity).

Patients and methods


Dutch speaking children between 8 and 18 years of age with a diagnosis of ESRD and treated with either haemodialysis or peritoneal dialysis for more than 3 months were included. The patients had to be able to perform exercise testing without musculoskeletal problems that could limit the exercise testing, and they had to be able to understand the exercise testing procedures. The eligible patients were identified by a senior paediatric nephrologist from each centre (JG, JN, KvH, ML).

A total of 25 children fulfilled the enrollment requirements and invited to participate. The parents of five children refused to participate due to personal or logistic reasons. Informed consent was obtained from all parents and also from the children if they were older than 12 years of age. The study was approved by the Medical Ethics Committee of all participating centres.

Patients were recruited from the paediatric nephrology outpatient clinic of the Wilhelmina Children’s Hospital Utrecht, Emma Children’s Hospital Amsterdam, Sophia Children’s Hospital Rotterdam and the University Hospital Antwerp, Belgium. All children were tested in their local hospital by the same research staff using the same mobile equipment to reduce bias (MvB, TT and RHE).

Twenty patients were included in this cross-sectional study. Eleven patients were treated with haemodialysis (mean age 14.1 ± 3.7 years), add nine were treated with peritoneal dialysis (mean age 14.0 ± 3.2 years). The medical histories of these children were obtained from respective hospital's patients’ medical records. A summary of their medical history is presented in Table 1. To eliminate any possible influence of a recent haemodialysis session on exercise capacity, patients were tested immediately prior to haemodialysis treatment or on an off-dialysis day.
Table 1

Medical history of the paediatric patients enrolled in the study


Patients (n = 20)

Time on dialysis (months)

27.5 ± 38.2

Haemodialysis (%)


Causes of renal failure


 Congenital structural abnormalities


 Hereditary renal disorders




 Systemic diseases






Hypertension medication


 ACE inhibitors


 ACE inhibitors + calcium blockers


 ACE inhibitors + calcium blockers + beta-blockers


 Calcium blockers


 Calcium blockers + beta-blockers




 Growth hormone




 Presence left ventricle hypertrophy


ACE, Angiotensin converting enzyme; rHuEPO, recombinant human erythropoietin

Data are given as mean ± SD or as n (%)


Clinical characteristics

Standing height and weight were measured to the nearest centimetre and 100 g, respectively, in a standardized method, with the patient not wearing heavy clothing and shoes. A body mass index (BMI) [weight (kg)/height (m2)] was then calculated from these values. Subcutaneous fat distribution was measured from skinfold measurements using Harpenden skinfold callipers. The measurements were taken at four sites, the triceps, biceps, subscapular and supra-iliacal [23], and converted to percentage body fat [24]. The systolic and diastolic blood pressure (mmHg) was measured in a sitting position using an oscillometric blood pressure device (Dinamap 8100 T; Critikon, Tampa, FL). The values of height, weight, BMI and blood pressure were compared with reference values for healthy, age- and gender-matched children [25, 26].

Six-minute walk test

The 6MWT was performed on a 20-m track in a straight hospital corridor. Patients were instructed to cover the largest possible distance in 6 min at a self-chosen walking speed. Turns were made on both ends of the 20-m track. The distance walked was recorded with a lap counter. At 1-min intervals, the patients were encouraged in a standardized way [17] and time, recorded with a stopwatch, was called. At the end of the test, the patient was asked to stand still, and the distance covered in the final partial lap was measured with measuring tape. The total distance covered was calculated by multiplying the number of laps (back and forth once) by 40 m and then adding the additional meters of the final partial lap. The total distance walked was rounded off to the nearest meter. The walking distance of our subjects was compared with predicted 6MWD using the gender-specific prediction equations from Geiger et al. [19] and the height-specific centile charts from Li et al. [20].

Progressive exercise test

Patients performed a progressive exercise test using an electronically braked cycle ergometer (Lode Corrival Pediatric; Lode BV, Groningen, the Netherlands). The test started with 1 min of unloaded cycling before the application of resistance to the ergometer. After this first minute, the work rate was increased with a constant increment of 10, 15 or 20 W each minute based on height according to the Godfrey protocol [27]. For patients <120 cm, the work rate was increased by 10 W/min; patients between 120 and 150 cm, by 15 W/min; patients >150 cm, by 20 W/min. This protocol continued until the patient voluntarily stopped because of exhaustion, despite strong verbal encouragement of the test-leader. During the test, subjects breathed through a facemask (Hans Rudolph, Kansas City, MO) connected to a calibrated respiratory gas analysis system (Cortex Metamax B3; Cortex Medical, Leipzig, Germany). Expired gas was passed through a flowmeter (Triple V volume transducer), an oxygen (O2) analyser and a carbon dioxide (CO2) analyser. The flowmeter and gas analysers were connected to a computer, which calculated breath-by-breath minute ventilation \( \left( {\mathop {\text{V}}\limits^\cdot {\text{E}}} \right) \), oxygen uptake \( \left( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_2}} \right) \), carbon dioxide output \( \left( {\mathop {\text{V}}\limits^\cdot {\text{C}}{{\text{O}}_2}} \right) \), and the respiratory exchange ratio \( \left( {{\text{RER}}; = \mathop {\text{V}}\limits^\cdot {\text{C}}{{\text{O}}_2}/\mathop {\text{V}}\limits^\cdot {{\text{O}}_2}} \right) \). The oxygen uptake eliciting the ventilatory threshold (VT) was determined by using the criteria of an increase in both the ventilatory equivalent of oxygen \( \left( {\mathop {\text{V}}\limits^\cdot {\text{E}}/\mathop {\text{V}}\limits^\cdot {{\text{O}}_2}} \right) \) and end-tidal pressure of oxygen (PETO2) with no increase in the ventilatory equivalent of carbon dioxide \( \left( {\mathop {\text{V}}\limits^\cdot {\text{E}}/\mathop {\text{V}}\limits^\cdot {\text{C}}{{\text{O}}_2}} \right) \) [28]. Heart rate was measured continuously during the maximal exercise test by using a heart rate monitor (Polar, Kempele, Finland). Maximal effort was defined as meeting one of the two criteria: heart rate >180 beats per minute or RER >1.0. Peak work rate (Wpeak) was defined as the highest work rate attained during the test.

Peak oxygen uptake \( \left( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \right) \) was defined as the average value for the last 30 s during the maximal exercise test. Relative \( \mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}} \) was calculated as absolute \( \mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}} \) divided by body mass. Predicted \( \mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}} \) and Wpeak values were obtained from established reference values for age- and gender matched healthy Dutch controls [29].

Muscle Strength

Muscle strength was measured with a hand-held dynamometer (Citec dynamometer CT 3001; C.I.T. Technics, Groningen, the Netherlands) in Newton (N). Muscle groups of the lower extremity assessed were knee flexors and extensors, hip flexors, and dorsiflexors of the ankle joint. Grip strength was also assessed as a indicator of global muscle strength. Maximum muscle strength was tested with the “break” method, in which the examiner gradually overcomes the muscle force of the patient and stops at the moment the extremity gives way.

Grip strength was measured using a Citec dynamometer equipped with an extension for measuring grip strength with the “make” method. Patients were sitting upright with the arm in shoulder adduction and at 70° of flexion. The elbow was flexed 30°. The extension was gripped as hard as possible for 3 s without pressing the instrument against the body and without touching the elbow to the body. Grip strength was included as a indicator of global muscle strength [30].

Every muscle group was measured three times, and the highest score was recorded.

Reference values for muscle strength were obtained from a study of healthy Dutch children [31].

Statistical analysis

Statistical analyses were performed with the use of the Statistical Package for the Social Sciences for Windows (ver. 13.0; SPSS, Chicago, IL). Prediction percentages were calculated using the score of each individual patient divided by the mean of the reference values for age and gender. Differences in 6MWT performance between children with ESRD and reference values was made using the Mann-Whitney Rank Sum Test. Differences in anthropometric variables, aerobic capacity, muscle strength and blood pressure between children with ESRD and reference values were made using independent samples t test.

The relationship between 6MWT performance and the following independent variables height, weight, BMI, haematocrit, hemoglobin, disease duration, muscle strength, VT and \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \) was made using univariate Pearson correlations and multiple backward linear regression analysis.

Independent variables that showed a significant univariate relationship (p < 0.05) with the dependent variables (% of predicted 6MWT performance according to the reference values of Li et al. [20] or Geiger et al. [19]) were entered in the final multiple linear regression model in which variables with p > 0.05 were excluded by means of the backward procedure. The α-level was set at p < 0.05 for all analyses.


Twenty children (13 boys, seven girls; mean age ± SD 14.1 ± 3.4 years) were enrolled in the study. Anthropometrics and clinical characteristics are illustrated in Table 2. Their mean body fat percentage was 16.4 ± 7.58% (range 5–31.1%). Compared with the reference values, children with ESRD had a significantly lower mean Z score for height and a lower Z score.
Table 2

Clinical characteristics of the study cohort


Mean ± SD (range)

Z scores ± SD

Height (m)

1.48 ± 0.20 (1.10-1.85)

-2.0 ± 1.4

Weight (kg)

44.6 ± 18.1 (19.5-81.0)

-0.83 ± 1.5

Body mass index (kg/m2)

19.4 ± 4.2 (15.0-31.0)

0.58 ± 2.0

Σ 4SF (mm)

36.2 ± 17.9 (15.0-71.6)

0.40 ± 2.0

Hemoglobin (mmol/l)

7.4 ± 1.0 (5.3-8.4)


Haematocrit (l/l)

0.35 ± 0.04 (0.25-0.42)


Systolic blood pressure (mmHg)

122.2 ± 18 (93-149)

1.4 ± 1.7

Diastolic blood pressure (mmHg)

76.1 ± 10.2 (63-101)

1.2 ± 0.9

Σ 4SF: sum of the four skin folds

In no case was it necessary to stop the tests prematurely, and there were no unexpected events during the tests. The 6MWT performance of the patients is shown in Table 3 and Fig. 1. Significantly reduced values were observed in the children with ESRD compared to the references values of Li et al. [20] (p < 0.0001) and Geiger et al. [19] (p < 0.0001). Both set of reference values gave percentages of predicted results and were highly correlated with each other (R = 0.975, p < 0.0001). There was no significant difference in 6MWT performance between the patients on haemodialysis and those on peritoneal dialysis (p = 0.7).
Table 3

Results of the six-minute walking test in children with end stage renal disease


Mean ± SD


6MWT—Distance (m)

554.6 ± 90.4


Predicted 6MWD—Li (m)

666.85 ± 30.9


Predicted 6MWD—Geiger (m)

663.26 ± 37.75


% predicted—Li

83.4% ± 11.04


% predicted—Geiger

83.3% ± 10.8


6MWD, 6-Minute walking test—distance; % predicted—Li, 6MWD as a percentage of the reference values obtained from Li et al. [20]; % predicted—Geiger, 6MWD as a percentage of the reference values obtained from Geiger et al. [19]

Fig. 1

The six-minute walking performance of children with end stage renal disease ESRD and healthy children (reference values according to Li et al. [20]) in relation to height. HD haemodialysis, PD peritoneal dialysis

The mean HRpeak was 168.67 ± 22.78 (range 131–201) beats/min during the progressive exercise test, which was significantly lower than the reference values (p < 0.001). Ten children did not meet the HR > 180 criterium. The RERpeak was 1.15 ± 0.15 (range 0.94–1.44), which was not significantly different from that of healthy subjects. Two children did not meet the RER > 1.0 criterium. One child did not meet both HRpeak and RERpeak criteria. The \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}}\)/kg of the patients was 30.9 ± 7.0 (range 19.0-43.0) ml/kg per minute, which is 66 ± 12% of the predicted value (p < 0.0001). Seventeen patients had a \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg lower than −2 SD. The Wpeak was 113.02 ± 48.1 (range 42.5-220) W, which is 54.4 ± 17% of the predicted (p < 0.0001). Only three patients had a Wpeak higher than −2 SD. The VT appeared at 32.7 ± 8.9% (21.3–51.3%) of the predicted \( \mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}} \), which is 55 ± 15% of the predicted compared to healthy subjects.

Muscle strength was, on average, 73 ± 29 (p < 0.001), 60 ± 16 (p < 0.0001), 62 ± 15 (p < 0.0001), 91 ± 25 (p = 0.49) and 62 ± 16% (p < 0.0001) of that predicted for grip strength, knee flexion, knee extension, foot dorsal flexion and hip flexion, respectively. Of the total study population (n = 20), 8, 11, 6, 2 and 8 patients had a muscle strength lower than −2 SD for grip strength, knee flexion, knee extension, foot dorsal flexion and hip flexion respectively.

Correlation analysis for height, weight, BMI, haematocrit, hemoglobin, muscle strength and aerobic fitness showed that only height, haematocrit, hemoglobin and VT were significantly associated with 6MWD when considered as a percentage of the reference values obtained by Li et al. [20] and Geiger et al. [19]. Six-minute walking performance was not associated with the use of antihypertensive medications, growth hormone and recombinant erythropoietin.

Height, haematocrit and VT were entered in the backward regression analysis (Table 4). Because haematocrit and haemoglobin were strongly correlated (r = 0.955, p < 0.0001), only haematocrit was entered in the regression analysis because it had slightly higher correlation coefficients with 6MWT performance compared to haemoglobin (% predicted according to Geiger et al.: r = 0.707 vs. r = 0.772; % predicted according to Li et al.: r = 0.651 vs. r = 0.717, respectively).
Table 4

Regression models of 6MWD as a percentage of the reference values according to Li et al. [20] and Geiger et al. [19]


Li et al. [20]


Geiger et al. [19]


B coefficient



B coefficient










haematocrit (%)







Height (m)









B, Regression coefficient; CI, confidence interval

Height and haematocrit were significant predictors of 6MWD performance using the Li et al. [20] reference values, while haematocrit was the only significant predictor of 6MWD performance using the Geiger et al. [19] reference values. Both models predicted approximately 60% of the variance in 6MWT performance.


The aim of the study reported here was to investigate the 6MWT performance of children with ESRD compared to reference values obtained in healthy children (discrimination validity) and, secondly, to study the relationship between 6MWT performance and anthropometric variables, clinical variables, aerobic fitness and muscle strength (construct validity).

We found that the 6MWT performance of the children with ESRD participating in our study was significantly lower than the reference values of Li et al. [20] and Geiger et al. [19], but they were comparable to those reported recently by Goldstein and Montgomery, who found a mean 6MWT distance of 538 m [4] in ten children receiving haemodialysis. Compared to other children with a chronic disease, our children with ESRD performed better that those with JIA (approx.72 % of predicted) and ambulatory children with spina bifida (approx. 60% of predicted), but they performed worse than those with haemophilia (90% of predicted) [9]. We are also able to confirm the previous finding of Hassan et al. [9] that the reference values of Li et al. [20] and Geiger et al. [19] are comparable when used in children with a chronic disease. There are, however, slight differences in the performance parameters of the 6MWT between these two studies. For example, Geiger et al. [19] used a measuring wheel as an incentive device during the test and a 20-m track, while Li et al. used a 30.5-m track. These differences do not seem to matter in terms of the applicability of the reference values.

The strongest predictors for 6MWT performance in our study were haematocrit and height. Height is not surprising since it is known from work with healthy children that height is a strong prediction of 6MWT performance. Consequently, both reference values are based on height (Li et al. [20]) or age and height (Geiger et al. [19]). In an earlier study in children with JIA, we also found that height was a significant predictor of 6-min walking distance [22]. A greater height will result in a larger leg length and hence a larger step length and walking speed.

Haematocrit was a strong predictor of 6MWT performance as well. This was surprising as haematocrit was not related to \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \) Wmax or muscle strength in our patients. However, a previous study in anaemic adult patients who were receiving haemodialysis, found that 6MWT distance increased with increasing haemoglobin concentration (correlation not reported) [32]. Moreover, increased exercise capacity \( \left( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \right) \) has been reported in adult and paediatric dialysis patients during their treatment of anaemia using erythropoietin [33, 34], although the \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \) response to increasing haematocrit in dialysis patients was clearly blunted compared to healthy subjects [35]. Based on this strong association with haematocrit, the 6MWT may be an interesting clinical outcome instrument in children with ESRD.

Surprisingly, 6MWT performance was not significantly related to lower extremity muscle strength, grip strength, \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \), Wpeak or VT. Previous studies in children with CF [12] and cardiorespiratory disease [11] reported significant associations between 6MWT performance and \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg or Wpeak. A study in children with JIA, however, reported no significant associations between 6MWT performance and \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg or Wpeak [13]. None of these studies reported on the relationship of BMWT performance and VT.

The children with ESRD in our study had a \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg of 31.3 ± 7.0 (range 19–43) ml/kg per minute. Our patients performed worse than those with CF, as reported in the study of Gulmans et al. [12] (\( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg 40.1 ± 9.1, range 27–60 ml/kg per minute), but better than the children with cardiopulmonary conditions in the study of Nixon et al. [11] (\( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg 19.0 ± 5.1, range 9.5–26.6 ml/kg per minute). This difference is not surprising as the patients in the latter study were candidates for lung and/or heart transplantations.

It is difficult to explain why the 6MWT performance was unrelated to \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \). Children with ESRD may have a different sensation of their perceived exertion during exercise because of abnormalities in their muscle metabolism [2]. As such, they may select a walking intensity based on oxygen delivery and/or a sensation of fatigue, which is unrelated to \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \) but which may be restricted by limitations in the skeletal muscle to extract oxygen [35].

Based on the significantly reduced values for aerobic fitness and muscle strength that we found in our study, a training programme may be indicated for children with ESRD. However, two recent pilot-studies have encountered significant challenges in patient adherence during such exercise training programmes [3, 4]. Based on the findings of Goldstein and Montgomery, the 6MWT seems to be a sensitive instrument for recording performance improvement as a result of exercise training in children with ESRD [4]. Moreover, based on it’s strong association with haematocrit, it may be an interesting clinical outcome measure for monitoring the clinical status (e.g. anaemia) of children with ESRD. However, more research is needed in this area.

Future studies using the 6MWT in children with ESRD should also determine the physiological strain of this test (i.e. heart rate, oxygen uptake etc) as well as the reproducibility of the test.

One limitation of this study is a possible selection bias. Patients who participate in exercise studies may be more interested in physical activity and may have a relatively higher exercise capacity than those who do not. It is known from the literature that self-selected subjects for an exercise test have a 5–10% higher fitness level than a randomly selected population [36]. In fact, the result may be somewhat worse in an average population of children with ESRD. Waever et al. [37] observed a \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg of approximately 25 ml/kg per minute in 12 children with ESRD on haemodialysis, and Painter et al. [2] reported a mean \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg of 27.9 ml/kg per minute in 15 children who underwent haemodialysis or peritoneal dialysis. Our patients scored, on average, a somewhat higher \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \)/kg (31.3 ml/kg per minute). This difference may be caused by a higher body mass in the patients of the other studies [2, 37], as increased body mass is probably due to a higher body fat percentage. Painter et al. [2] reported a mean body fat percentage of 26.9%, which is quite a lot higher that the percentage body fat in our subjects (16.4%).

In conclusion, we found that children with ESRD performed significantly poorer on the 6MWT in comparison with reference values and that their performance was significantly associated with haematocrit and height. The two sets of reference values used here provided similar results. The 6MWT may be a useful instrument for monitoring clinical status in children with ESRD, however it cannot substitute for other fitness tests, such as a progressive exercise test to measure \( {\mathop {\text{V}}\limits^\cdot {{\text{O}}_{\text{2peak}}}} \) or a muscle strength test.



This study was supported by the Nierstichting Nederland (Dutch Kidney Foundation) (grant nr. KI30). We thank all of the children and their parents who participated in this study.

Open Access

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Copyright information

© The Author(s) 2009

Authors and Affiliations

  • Tim Takken
    • 1
  • Raoul Engelbert
    • 1
    • 2
  • Monique van Bergen
    • 1
  • Jaap Groothoff
    • 3
  • Jeroen Nauta
    • 4
  • Koen van Hoeck
    • 5
  • Marc Lilien
    • 6
  • Paul Helders
    • 1
  1. 1.Department of Pediatric Physical Therapy & Exercise Physiology, Wilhelmina Children’s HospitalUniversity Medical Centre UtrechtUtrechtThe Netherlands
  2. 2.University of Applied Sciences Amsterdam, Hogeschool van AmsterdamEducation of Physical TherapyAmsterdamThe Netherlands
  3. 3.Department of Pediatric Nephrology, Emma Children’s HospitalAcademic Medical CentreAmsterdamThe Netherlands
  4. 4.Department of Pediatric Nephrology, Sophia’s Children HospitalErasmus Medical CentreRotterdamThe Netherlands
  5. 5.Department of Pediatric NephrologyUniversity Hospital AntwerpAntwerpBelgium
  6. 6.Department of Pediatric Nephrology, Wilhelmina Children’s HospitalUniversity Medical Centre UtrechtUtrechtThe Netherlands

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