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Pediatric Nephrology

, Volume 24, Issue 9, pp 1691–1698 | Cite as

T regulatory cell function in idiopathic minimal lesion nephrotic syndrome

  • Carlos Araya
  • Leila Diaz
  • Clive Wasserfall
  • Mark Atkinson
  • Wei Mu
  • Richard Johnson
  • Eduardo Garin
Original Article

Abstract

The purpose of this study was to test the hypothesis that, in idiopathic minimal lesion nephrotic syndrome (IMLNS), the T regulatory (T reg) cell suppressor mechanism is deficient, thereby enhancing cytokine release by T effector cells. Twenty-one patients with IMLNS, eight healthy controls and two patients with nephrotic syndrome and membranoproliferative glomerulonephritis were studied. The percentage of T reg cells was similar in the healthy controls and in patients with IMLNS in relapse or in remission. Thymidine incorporation in autologous T effector cells, as well as expression of the regulatory cytokine interleukin (IL)-10, was significantly reduced in patients in relapse when compared with patients in remission and healthy subjects. IL-2 expression was also reduced in patients in relapse but did not achieve statistical significance. In a different set of experiments, T cells, from subjects with IMLNS in remission, when stimulated with antiCD3-antiCD28 antibodies, secreted increased levels of cytokines. No such increase in cytokines was observed when cells from healthy controls were stimulated with same mitogen. The impaired T reg cell function observed in these patients may have pathogenic and therapeutic implications, because it could explain the persistence of the proposed pathogenic cytokines observed in the patients with IMLNS.

Keywords

Minimal lesion nephrotic syndrome T regulatory cell Cytokines 

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Copyright information

© IPNA 2009

Authors and Affiliations

  • Carlos Araya
    • 1
  • Leila Diaz
    • 1
  • Clive Wasserfall
    • 2
  • Mark Atkinson
    • 2
  • Wei Mu
    • 3
  • Richard Johnson
    • 3
  • Eduardo Garin
    • 1
    • 4
  1. 1.Department of PediatricsUniversity of FloridaGainesvilleUSA
  2. 2.Department of PathologyUniversity of FloridaGainesvilleUSA
  3. 3.Department of MedicineUniversity of FloridaGainesvilleUSA
  4. 4.Division of Pediatric NephrologyGainesvilleUSA

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