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Pediatric Nephrology

, Volume 23, Issue 2, pp 257–262 | Cite as

Histochemical and immunohistochemical study of the glomerular development in human fetuses

  • Mara Lúcia Fonseca Ferraz
  • Aline Mara dos Santos
  • Camila Lourencini Cavellani
  • Renata Calciolari Rossi
  • Rosana Rosa Miranda Corrêa
  • Marlene Antônia dos Reis
  • Vicente de Paula Antunes Teixeira
  • Eumenia Costa da Cunha Castro
Original Article

Abstract

Few studies exist that establish the normal morphological patterns of glomerular development, though this is one of the organs that continues to evolve morphologically during the postnatal period up to 4 weeks after birth. In our study one kidney from each autopsy of a total of 86 autopsies was analyzed [15 weeks to 40 weeks of gestational age (GA)]. We examined the variation in the nephrogenic zone thickness, the area and diameter of the glomerular tuft, the area and diameter of the glomerular capsule, and the immunohistochemical markers, anti-CD31 and anti-CD34 antibodies, which accompany the development of the glomerular microvasculature. Periodic acid–methenamine silver (PAMS) stain was used for the morphological and morphometrical analyses, and it was particularly useful in fetuses in which autolysis had occurred. The length of the nephrogenic zone (NZ) decreased with the increase of the GA (P < 0.001) according to the formula: \({\text{GA}} = 36.5 - {\left( {0.05 \times {\text{length of NZ}}} \right)}\). The areas of the Bowman capsule (P < 0.0001), the capillary tuft (P < 0.0001), and the capillary tuft diameter (P = 0.00393) of the intermediary glomeruli increased with the advance of GA, with a positive significant correlation. The same parameters of the juxtamedullary and superficial glomeruli had no correlation with the advance of GA. The cells of the primary structures in the “S” shape of the primitive nephrons were negative for CD31 and CD34. Staining for both antibodies was found, for all GAs, in the endothelial cells of the mature glomeruli tuft and in the renal interstitial vessels. The data obtained in this work contribute to the evaluation of renal maturity in autopsied fetuses and are particularly important in fetuses when autolysis has occurred, to which the parameters used in this study can also be applied. The establishment of normal morphometric and immunohistochemical parameters for the evaluation of renal maturity increases the diagnostic precision of renal pathological alterations in aborted material and perinatal autopsy.

Keywords

Kidney Glomerulogenesis Gestational age Perinatal autopsy 

Notes

Acknowledgments

Financial Support was received from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG).

References

  1. 1.
    Callaghan WM, MacDorman MF, Rasmussen SA, Qin C, Lackritz FM (2006) The contribution of preterm birth to infant mortality rates in the United States. Pediatrics 118:1566–1573PubMedCrossRefGoogle Scholar
  2. 2.
    Deulofeut R, Critz A, Adams-Chapman I, Sola A (2006) Avoiding hyperoxia in infants ≤1250 g is associated with improved short- and long-term outcomes. J Perinatol 26:700–705PubMedCrossRefGoogle Scholar
  3. 3.
    Kelly MM (2006) Primary care issues for the healthy premature infant. J Pediatr Health Care 20:293–329PubMedCrossRefGoogle Scholar
  4. 4.
    Zecca E, Luca D, Costa S, Marras M, de Turris P, Romagnoli C (2006) Delivery room strategies and outcomes in preterm infants with gestational age 24–28 weeks. J Matern Fetal Neonatal Med 19:569–574PubMedCrossRefGoogle Scholar
  5. 5.
    Roy KK, Baruah J, Kumar S, Malhotra N, Deorari AK, Sharma JB (2006) Maternal antenatal profile and immediate neonatal outcome in VLBW and ELBW babies. Indian J Pediatr 73:669–673PubMedGoogle Scholar
  6. 6.
    Georgsdottir I, Saemundsen E, Simonardottir I, Halldorsson JG, Egilson ST, Leosdottir T, Ingvarsdottir B, Sindrason E, Dagbjartsson A (2003) Extremely low birthweight infants in Iceland. Health and development. Laeknabladid 89:575–581PubMedGoogle Scholar
  7. 7.
    Dakovic-Bjelakovic M, Vlajkovic S, Cukuranovic R, Antic S, Bjelakovic G, Mitic D (2006) Changes of the glomerular size during the human fetal kidney development. Srp Arh Celok Lek 134:33–39PubMedGoogle Scholar
  8. 8.
    Hawkins MR, Diehl-Svrjcek B, Dunbar LJ (2006) Caring for children with special healthcare needs in the managed care environment. Lippincotts Case Manag 11:21–223Google Scholar
  9. 9.
    Naruse K, Fujieda M, Miyazaki E, Hayashi Y, Toi M, Fukui T, Kuroda N, Hiroi M, Kurashige T, Enzan H (2000) An immunohistochemical study of developing glomeruli in human fetal kidneys. Kidney Int 57:1836–1846PubMedCrossRefGoogle Scholar
  10. 10.
    Bernhardt WM, Schmitt R, Rosenberger C, Münchenhagen PM, Gröne HJ, Frei U, Warnecke C, Bachmann S, Wiesener MS, Willam C, Eckardt KU (2006) Expression of hypoxia inducible transcription factors in developing human and rat kidneys. Kidney Int 69:114–122PubMedCrossRefGoogle Scholar
  11. 11.
    Gomes RA, Norwood VF (1999) Recent advances in renal development. Curr Opin Pediatr 11:135–140CrossRefGoogle Scholar
  12. 12.
    Osathanondh V, Potter EL (1963) Development of human kidney as shown by microdissection. Arch Pathol 76:290–302PubMedGoogle Scholar
  13. 13.
    Osathanondh V, Potter EL (1966) Development of human kidney as shown by microdissection. Arch Pathol 82:403–411PubMedGoogle Scholar
  14. 14.
    Potter EL (1965) Development of the human glomerulus. Arch Pathol 80:241–255PubMedGoogle Scholar
  15. 15.
    Hyink DP, Abrahamson DR (1995) Origin of the glomerular vasculature in the developing kidney. Semin Nephrol 15:300–314PubMedGoogle Scholar
  16. 16.
    Mattot V, Moons L, Lupu F, Chernavvsky D, Gómez RA, Collen D, Carmeliet P (2002) Loss of the VEGF164 isoforms impairs postnatal glomerular angiogenesis and renal arteriogenesis in mice. J Am Soc Nephrol 13:1548–1560PubMedCrossRefGoogle Scholar
  17. 17.
    Streeter GL (1920) Weight, sitting height, head size, foot length and menstrual age of human embryo. Contrib Embryol Carnegie Inst 11:143–170Google Scholar
  18. 18.
    Sagi J, Vagman I, David MP, Van Dongen LG, Goudie E, Butterworth A, Jacobson MJ (1987) Fetal kidney size related to gestational age. Gynecol Obstet Invest 23:1–4PubMedCrossRefGoogle Scholar
  19. 19.
    Barbet JP, Houette A, Barres D, Durigon M (1988) Histological assessment of gestational age in human embryos and fetuses. Am J Forensic Med Pathol 9:40–44PubMedCrossRefGoogle Scholar
  20. 20.
    Kumar K, Pillay VV (1996) Estimation of fetal age by histological study of kidney. Med Sci Law 36:226–230PubMedGoogle Scholar
  21. 21.
    Gasser B, Mauss Y, Ghnassia JP (1993) A quantitative study of normal nephrogenesis in the human fetus: its implication in the natural history of kidney changes due to low obstructive uropathies. Fetal Diagn Ther 8:371–384PubMedCrossRefGoogle Scholar
  22. 22.
    Shimada K, Matsumoto F, Tohda A, Ureda M (2003) Histological study of fetal kidney with urethral obstruction and vesicoureteral reflux: a consideration on the etiology of congenital reflux nephropathy. Int J Urol 10:518–524PubMedCrossRefGoogle Scholar
  23. 23.
    Hume R, Coughtrie MW, Burchell B (1995) Differential localization of UDP-glucuronosyltransferase in kidney during human embryonic and fetal development. Arch Toxicol 69:242–247PubMedCrossRefGoogle Scholar
  24. 24.
    Manalich R, Reyes L, Herrera M, Melendi C, Fundora I (2000) Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study. Kidney Int 58:77–773CrossRefGoogle Scholar
  25. 25.
    Rodriguez MM, Gomez AH, Abitbol CL, Chandar JJ, Duara S, Zilleruelo GE (2004) Histomorphometric analysis of postnatal glomerulogenesis in extremely preterm infants. Pediatr Dev Pathol 7:17–25PubMedCrossRefGoogle Scholar
  26. 26.
    Pustaszeri MP, Seelentag W, Bosman FT (2006) Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and fli-1 in normal human tissues. J Histochem Cytochem 54:385–395CrossRefGoogle Scholar
  27. 27.
    Ricono JM, Xu Y, Arar M, Jin DC, Barnes JL, Abboud HE (2003) Morphological insight into the origin of glomerular endothelial and mesangial cells and their precursors. J Histochem Cytochem 51:141–150PubMedGoogle Scholar
  28. 28.
    Koklanaris N, Nwachukwu JC, Huang SJ, Guller S, Karpisheva K, Garabedian M, Lee MJ (2006) First-trimester trophoblast cell model gene response to hypoxia. Am J Obstet Gynecol 194:687–693PubMedCrossRefGoogle Scholar

Copyright information

© IPNA 2007

Authors and Affiliations

  • Mara Lúcia Fonseca Ferraz
    • 1
  • Aline Mara dos Santos
    • 1
  • Camila Lourencini Cavellani
    • 1
  • Renata Calciolari Rossi
    • 1
  • Rosana Rosa Miranda Corrêa
    • 1
  • Marlene Antônia dos Reis
    • 1
  • Vicente de Paula Antunes Teixeira
    • 1
  • Eumenia Costa da Cunha Castro
    • 1
  1. 1.Biological Sciences Department, General Pathology DisciplineTriângulo Mineiro Federal UniversityUberabaBrazil

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