Pediatric Nephrology

, Volume 20, Issue 1, pp 52–55 | Cite as

Serum VCAM-1, ICAM-1, and L-selectin levels in children and young adults with chronic renal failure

  • Kinga Musiał
  • Danuta Zwolińska
  • Dorota Polak-Jonkisz
  • Urszula Berny
  • Krystyna Szprynger
  • Maria Szczepańska
Original Article


Children and young adults with chronic renal failure (CRF) present with an impaired immune response. Our aim was to analyze whether leukocyte migration, determined by adhesion molecules, is disturbed in the course of CRF, hemodialysis (HD), and peritoneal dialysis (PD). Soluble (s) VCAM-1, ICAM-1, and L-selectin serum levels were evaluated by ELISA in 15 patients with CRF, 22 patients on cuprophane membrane HD, 24 patients on PD, and in 15 controls. The sVCAM-1 levels in all groups were significantly elevated compared with controls. The levels in HD patients were higher than in CRF patients (P <0.05), while levels in PD patients were higher than in CRF and HD (P <0.001 and P <0.01, respectively). The sICAM-1 concentrations in CRF and PD patients were significantly elevated compared with controls (P <0.001 and P <0.0001, respectively); in PD patients sICAM-1 levels were higher than in HD patients (P <0.001), but there were no differences between other groups. sL-selectin levels were decreased in all groups compared with controls. The levels in HD patients were the lowest and the differences, compared with CRF and PD patients, were significant (P <0.05 and P <0.01, respectively). Children and young adults with CRF and on maintenance dialysis have altered concentrations of soluble adhesion molecules, resulting from either inadequate clearance or disturbed synthesis and release. The differences in sVCAM-1 levels between CRF and both groups of patients on dialysis, as well as the differences in sL-selectin concentrations between HD and CRF patients, indicate that these disturbances are aggravated by maintenance dialysis, particularly HD.


Soluble adhesion molecules Young adults Chronic renal failure Hemodialysis Peritoneal dialysis 



A part of this work was presented at the ESPN Congress in Bilbao in 2002.


  1. 1.
    Petruzzelli L, Takami M, Humes HD (1999) Structure and function of cell adhesion molecules. Am J Med 106:467–476CrossRefPubMedGoogle Scholar
  2. 2.
    Noris M, Remuzzi G (1995) New insights into circulating cell-endothelium interactions and their significance for glomerular pathophysiology. Am J Kidney Dis 26:541–548PubMedGoogle Scholar
  3. 3.
    Carlos TM, Harlan JM (1994) Leukocyte-endothelial adhesion molecules. Blood 84:2068–2101PubMedGoogle Scholar
  4. 4.
    Springer TA (1997) Traffic signals on endothelium for lymphocyte recirculation and leukocyte emigration. In: Leendert CP, Issekutz TB (eds) Adhesion molecules in health and disease. Marcel Dekker, New York, pp 1–54Google Scholar
  5. 5.
    Pozzi A, Zent R (2003) Integrins: sensors of extracellular matrix and modulators of cell function. Nephron Exp Nephrol 94:e77–e84CrossRefPubMedGoogle Scholar
  6. 6.
    Pigott R, Dillon LP, Hemingway IH, Gearing AJ (1992) Soluble forms of E-selectin, ICAM-1 and VCAM-1 are present in the supernatant of cytokine activated cultured endothelial cells. Biochem Biophys Res Commun 187:584–589PubMedGoogle Scholar
  7. 7.
    Leeuwenberg JFM, Smeets EF, Neefjes JJ, Shaffer AA, Cinek T, Jeunhomme TMAA, Ahern TJ, Buurman WA (1992) E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77:543–549PubMedGoogle Scholar
  8. 8.
    Gearing AJH, Newman W (1993) Circulating adhesion molecules in disease. Immunol Today 14:506–512CrossRefPubMedGoogle Scholar
  9. 9.
    Schleiffenbaum B, Spertini O, Tedder TF (1992) Soluble L-selectin is present in human plasma at high levels and retains functional activity. J Cell Biol 119:229–238CrossRefPubMedGoogle Scholar
  10. 10.
    Gamble JR, Skinner MP, Berndt MC, Vadas MA (1990) Prevention of activated neutrophil adhesion to endothelium by soluble adhesion protein GMP-140. Science 249:414–417PubMedGoogle Scholar
  11. 11.
    Ara J, Mirapeix E, Arrizabalaga P, Rodriguez R, Ascaso C, Abellana R, Font J, Darnell A (2001) Circulating soluble adhesion molecules in ANCA-associated vasculitis. Nephrol Dial Transplant 16:276–285CrossRefPubMedGoogle Scholar
  12. 12.
    Kawabata K, Nagake Y, Shikata K, Makino H, Ota Z (1996) The changes of Mac-1 and L-selectin expression on granulocytes and soluble L-selectin level during hemodialysis. Nephron 73:573–579PubMedGoogle Scholar
  13. 13.
    Dou L, Brunet P, Dignat-George F, Sampol J, Berland Y (1998) Effect of uremia and hemodialysis on soluble L-selectin and leukocyte surface CD11b and L-selectin. Am J Kidney Dis 31:67–73PubMedGoogle Scholar
  14. 14.
    Bonomini M, Reale M, Santarelli P, Stuard S, Settefrati N, Albertazzi A (1998) Serum levels of soluble adhesion molecules in chronic renal failure and dialysis patients. Nephron 79:399–407CrossRefPubMedGoogle Scholar
  15. 15.
    Mrowka C, Heintz B, Sieberth HG (1999) Is dialysis membrane type responsible for increased circulating adhesion molecules during chronic hemodialysis? Clin Nephrol 52:312–321PubMedGoogle Scholar
  16. 16.
    Stenvinkel P, Lindholm B, Heimburger M, Heimburger O (2000) Elevated serum levels of soluble adhesion molecules predict death in pre-dialysis patients: association with malnutrition, inflammation, and cardiovascular disease. Nephrol Dial Transplant 15:1624–1630CrossRefPubMedGoogle Scholar
  17. 17.
    Descamps-Latscha B, Herbelin A, Nguyen AT, Roux-Lombard P, Zingraff J, Moynot J, Verger C, Dahmane D, Groote D de, Jungers P, Dayer JM (1995) Balance between IL-1β, TNF-α and their specific inhibitors in chronic renal failure and maintenance dialysis. Relationships with activation markers of T cells, B cells and monocytes. J Immunol 154:882–892PubMedGoogle Scholar
  18. 18.
    Zwolińska D, Medyńska A, Szprynger K, Szczepańska M (2000) Serum concentration of IL-2, IL-6, TNF-alpha and their soluble receptors in children on maintenance hemodialysis. Nephron 86:441–446CrossRefPubMedGoogle Scholar
  19. 19.
    Musiał K, Zwolińska D, Polak-Jonkisz D, Berny U, Szprynger K, Szczepańska M (2004) Soluble adhesion molecules in children and young adults on hemodialysis. Pediatr Nephrol 19:332–336CrossRefPubMedGoogle Scholar
  20. 20.
    Boulanger E, Wautier MP, Wautier JL, Boval B, Panis Y, Wernert N, Danze PM, Dequiedt P (2002) AGEs bind to mesothelial cells via RAGE and stimulate VCAM-1 expression. Kidney Int 61:148–156CrossRefPubMedGoogle Scholar
  21. 21.
    Rothlein R, Mainolfi EA, Czajkowski M, Marlin SD (1991) A form of circulating ICAM-1 in human serum. J Immunol 147:3788–3793PubMedGoogle Scholar
  22. 22.
    Vanholder R, Van Loo A, Dhondt AM, De Smet R, Ringoir S (1996) Influence of uremia and hemodialysis on host defence and infection. Nephrol Dial Transplant 11:593–598PubMedGoogle Scholar
  23. 23.
    Haag-Weber M, Horl WH (1996) Dysfunction of polymorphonuclear leukocytes in uremia. Semin Nephrol 16:192–201PubMedGoogle Scholar
  24. 24.
    Dadfar E, Lundahl J, Jacobson SH (2004) Monocyte adhesion molecule expression in interstitial inflammation in patients with renal failure. Nephrol Dial Transplant 19:614–622CrossRefPubMedGoogle Scholar

Copyright information

© IPNA 2004

Authors and Affiliations

  • Kinga Musiał
    • 1
  • Danuta Zwolińska
    • 1
    • 3
  • Dorota Polak-Jonkisz
    • 1
  • Urszula Berny
    • 1
  • Krystyna Szprynger
    • 2
  • Maria Szczepańska
    • 2
  1. 1.Department of Pediatric NephrologyMedical UniversityWrocławPoland
  2. 2.Department of Pediatrics, Clinic of Nephrology, Endocrinology and Metabolic Diseases of ChildhoodSilesian School of MedicineZabrzePoland
  3. 3.Department of Pediatric NephrologyWrocław Medical UniversityPoland

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