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Pediatric Nephrology

, Volume 19, Issue 11, pp 1212–1218 | Cite as

Postnatal renal development of rats from mothers that received increased sodium intake

  • Ana Paula C. Balbi
  • Roberto S. Costa
  • Terezila M. Coimbra
Original Article

Abstract

The newborn rat kidney is not fully developed until approximately 12 days after birth. Several lines of evidence suggest that angiotensin II (AII) participates in the postnatal development of the kidney. The aim of the present study was to analyze proliferating cell nuclear antigen (PCNA), fibronectin, α-smooth muscle-actin (α-SM-actin), and AII expression in renal cortex during development in rats born to mothers that received a normal (control) or increased (experimental) sodium intake during pregnancy. Ninety Wistar rats aged 1, 7, 15, and 30 days from the control and experimental groups were killed and the kidneys removed for histological and immunohistochemical studies. The results showed higher fibronectin, α-SM-actin, PCNA, and AII expression in the glomerular and tubulointerstitial areas of the renal cortex of 1- and 7-day-old animals, which decreased with renal development. The animals from the experimental group showed at 1 day of age a decrease in α-SM-actin, fibronectin, PCNA, and AII expression compared with controls of the same age ( P <0.05). In conclusion, our data show that increased sodium intake during pregnancy induces a reduction of α-SM-actin, fibronectin, and PCNA expression in the renal cortex tubulointerstitium and glomeruli of neonatal rats. These alterations may be related to the decrease of AII expression also observed in the renal cortex from these animals.

Keywords

Renal development Angiotensin II Sodium intake Cell proliferation Extracellular matrix 

Notes

Acknowledgements

The authors thank Cleonice G.A. da Silva, Erika Delloiagono, and Rubens Fernando de Melo for expert technical assistance. Ana Paula C. Balbi was a recipient of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, DF, Brazil, fellowship, and Dr. Roberto Silva Costa and Dr. Terezila Machado Coimbra are recipients of Conselho Nacional de Desenvolvimento Científico e Tecnológico, DF, Brazil, fellowships. These results were presented in abstract form at the American Society of Nephrology Meeting, Philadelphia, Pa., November 2002.

References

  1. 1.
    Nigam SK, Aperia AC, Brenner BM (1996) Development and maturation of the kidney. In: Brenner BM, Rector FC (eds): The kidney. Saunders, Philadelphia, pp 72–98Google Scholar
  2. 2.
    Reeves W, Caulfield JP, Farquhar MG (1978) Differentiation of epithelial foot processes and filtration slits: sequential appearance of occluding junctions, epithelial polyanion, and slit membranes in developing glomeruli. Lab Invest 39:90–100PubMedGoogle Scholar
  3. 3.
    Roberts AB, McCune BK, Sporn MB (1992) TGF-β: regulation of extracellular matrix. Kidney Int 41:557–559PubMedGoogle Scholar
  4. 4.
    Thiery JP, Duband JL, Dufour S, Savagner P, Imhof BA (1989) Role of fibronectin in embryogenesis. In: Mosher DF (ed) Biology of extracellular matrix: fibronectin. Academic Press, San Diego, pp 181–212Google Scholar
  5. 5.
    Kagami S, Border WA, Miller DE, Noble NA (1994) Angiotensin II stimulates extracellular matrix protein synthesis through induction of transforming growth factor-β expression in rat glomerular mesangial cells. J Clin Invest 93:2431–2437PubMedGoogle Scholar
  6. 6.
    Bagby SP, Kirk EA, Mitchell LH, O’Reilly MM, Holden WE, Stenberg PE, Bakke AG (1993) Proliferative synergy of angiotensin II and EGF in porcine aortic vascular smooth muscle cells. Am J Physiol 265:F239–F249Google Scholar
  7. 7.
    Fernandez LA, Twickler J, Mead A (1985) Neovascularization produced by angiotensin II. J Lab Clin Med 105:141–145PubMedGoogle Scholar
  8. 8.
    Gomez RA (1990) Molecular biology of components of the renin-angiotensin system during development. Pediatr Nephrol 4:421–423PubMedGoogle Scholar
  9. 9.
    Gomez RA, Lynch KR, Sturgill BC, Elwood JP, Chevalier RL, Carey RM, Peach MJ (1989) Distribution of renin mRNA and its protein in the developing kidney. Am J Physiol 257: F850–F858Google Scholar
  10. 10.
    Millan MA, Carvallo P, Izumi S, Zemel S, Catt KJ, Aguilera G (1989) Novel sites of expression of functional angiotensin II receptors in the late gestation fetus. Science 244:1340–1342PubMedGoogle Scholar
  11. 11.
    Tufro-McReddie A, Harrison JK, Everett AD, Gomez RA (1993) Ontogeny of type 1 angiotensin II receptor gene expression in the rat. J Clin Invest 91:530–537PubMedGoogle Scholar
  12. 12.
    Grady EF, Sechi LA, Griffin CA, Schambelan M, Kalinyak JE (1991) Expression of AT2 receptors in the developing rat fetus. J Clin Invest 88:921–933PubMedGoogle Scholar
  13. 13.
    Du Y, Yao A, Guo D, Inagami T, Wang DH (1995) Differential regulation of angiotensin II receptors subtypes in rat kidney by low dietary sodium. Hypertension 25:872–877PubMedGoogle Scholar
  14. 14.
    Coimbra TM, Janssen U, Gröne HJ, Ostendorf T, Kunter U, Schmidt H, Brabant G, Floege J (2000) Early events leading to renal injury in obese Zucker (fatty) rats with type II diabetes. Kidney Int 57:167–182CrossRefPubMedGoogle Scholar
  15. 15.
    Kliem V, Johnson RJ, Alpers CE, Yoshimura A, Couser WG, Koch KM, Floege J (1996) Mechanisms involved in the pathogenesis of tubulointerstitial fibrosis in 5/6-nephrectomized rats. Kidney Int 49:666–678PubMedGoogle Scholar
  16. 16.
    Stambe C, Atkins RC, Hill PA, Nikolic-Paterson DJ (2003) Activation and cellular localization of the p38 and JNK MAPK pathways in rat crescentic glomerulonephritis. Kidney Int 64:2121–2132CrossRefPubMedGoogle Scholar
  17. 17.
    Hartree EF (1972) Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem 48:422–427PubMedGoogle Scholar
  18. 18.
    Fuehr Y, Kaczmarczk Y, Kruttgen GD (1955) Eine einfache colorimetrische Methode zur Inulin-Bestimmung für Nieren-Clearance-Untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin Wochenschr 33:729–730PubMedGoogle Scholar
  19. 19.
    Alpers CE, Hudkins KL, Gown AM, Johnson RJ (1992) Enhanced expression of “muscle-specific” actin in glomerulonephritis. Kidney Int 41:1134–1142PubMedGoogle Scholar
  20. 20.
    El Nahas AM, Muchaneta-Kubara EC, Zhang G, Adam A, Goumenos D (1996) Phenotypic modulation of renal cells during experimental and clinical renal scarring. Kidney Int 54:S23–S27Google Scholar
  21. 21.
    Geleilete TJ, Melo GC, Costa RS, Volpini RA, Soares TJ, Coimbra TM (2002) Role of myofibroblasts, macrophages, transforming growth factor-beta, endothelin, angiotensin-II, and fibronectin in the progression of tubulointerstitial nephritis induced by gentamicin. J Nephrol 15:633–642PubMedGoogle Scholar
  22. 22.
    Johnson RJ, Alpers CE, Yoshimura A, Lombardi D, Pritzl P, Floege J, Schwartz SM (1992) Renal injury from angiotensin II-mediated hypertension. Hypertension 19:464–474PubMedGoogle Scholar
  23. 23.
    Chen Y, Lasaitiene D, Gabrielsson BG, Carlsson LM, Billig H, Carlsson B, Marcussen N, Sun XF, Friberg P (2004) Neonatal losartan treatment suppresses renal expression of molecules involved in cell-cell and cell-matrix interactions. J Am Soc Nephrol 15:1232–1243Google Scholar
  24. 24.
    Johnson RJ, Iida H, Alpers CE, Majesky MW, Schwartz SM, Pritzl P, Gordon K, Gown AM (1991) Expression of smooth muscle cell phenotype by rat mesangial cells in immune complex nephritis. Alpha-smooth muscle actin is a marker of mesangial cell proliferation. J Clin Invest 87:847–858PubMedGoogle Scholar
  25. 25.
    Carey AV, Carey RM, Gomez RA (1992) Expression of alpha-smooth muscle actin in the developing kidney vasculature. Hypertension 19 [Suppl]:168–175Google Scholar
  26. 26.
    Naruse K, Fujieda M, Miyasaki E, Hayashi Y, Toi M, Fukui T, Kuroda N, Hiroi M, Kurashige T, Enzan H (2000) An immunohistochemical study of developing glomeruli in human fetal kidneys. Kidney Int 57:1836–1846CrossRefPubMedGoogle Scholar
  27. 27.
    Omori S, Hida M, Ishikura K, Kuramochi S, Awazu M (2000) Expression of mitogen-activated protein kinase family in rat renal development. Kidney Int 58:27–37CrossRefPubMedGoogle Scholar
  28. 28.
    Hsuch WA, Do YS, Anderson PW, Law RE (1995) Angiotensin II in cell growth and matrix production. Adv Exp Med Biol 377:217–223PubMedGoogle Scholar
  29. 29.
    McCausland JE, Ryan GB, Alcorn D (1996) Angiotensin converting enzyme inhibition in the postnatal rat results in decreased cell proliferation in the renal outer medulla. Clin Exp Pharmacol Physiol 23:552–554PubMedGoogle Scholar
  30. 30.
    Guron G, Friberg P (2000) An intact renin angiotensin system is a prerequisite for normal renal development. J Hypertens 18:123–137CrossRefPubMedGoogle Scholar
  31. 31.
    Nicolantonio RD, Spargo S, Morgan TO (1987) Prenatal high salt diet increases blood pressure and salt retention in spontaneously hypertensive rat. Clin Exp Pharmacol Physiol 14:233–235PubMedGoogle Scholar
  32. 32.
    Silva AA, Noronha IL, Oliveira IB de, Malheiros DMC, Heimann JC (2003) Renin-angiotensin system function and blood pressure in adult rats after perinatal salt overload. Nutr Metab Cardiovasc Dis 13:133–139PubMedGoogle Scholar
  33. 33.
    Hazon N, Parker C, Leonard R, Henderson IW (1988) Influence of an enriched sodium chloride regime during gestation and suckling and post-natally on the ontogeny of hypertension in the rat. J Hypertens 6:517–524PubMedGoogle Scholar

Copyright information

© IPNA 2004

Authors and Affiliations

  • Ana Paula C. Balbi
    • 1
  • Roberto S. Costa
    • 2
  • Terezila M. Coimbra
    • 1
  1. 1.Department of Physiology, Faculty of MedicineUniversity of Sao PauloSao PauloBrazil
  2. 2.Department of Pathology, Faculty of MedicineUniversity of Sao PauloSao PauloBrazil

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