Pediatric Nephrology

, Volume 19, Issue 11, pp 1212–1218 | Cite as

Postnatal renal development of rats from mothers that received increased sodium intake

  • Ana Paula C. Balbi
  • Roberto S. Costa
  • Terezila M. Coimbra
Original Article


The newborn rat kidney is not fully developed until approximately 12 days after birth. Several lines of evidence suggest that angiotensin II (AII) participates in the postnatal development of the kidney. The aim of the present study was to analyze proliferating cell nuclear antigen (PCNA), fibronectin, α-smooth muscle-actin (α-SM-actin), and AII expression in renal cortex during development in rats born to mothers that received a normal (control) or increased (experimental) sodium intake during pregnancy. Ninety Wistar rats aged 1, 7, 15, and 30 days from the control and experimental groups were killed and the kidneys removed for histological and immunohistochemical studies. The results showed higher fibronectin, α-SM-actin, PCNA, and AII expression in the glomerular and tubulointerstitial areas of the renal cortex of 1- and 7-day-old animals, which decreased with renal development. The animals from the experimental group showed at 1 day of age a decrease in α-SM-actin, fibronectin, PCNA, and AII expression compared with controls of the same age ( P <0.05). In conclusion, our data show that increased sodium intake during pregnancy induces a reduction of α-SM-actin, fibronectin, and PCNA expression in the renal cortex tubulointerstitium and glomeruli of neonatal rats. These alterations may be related to the decrease of AII expression also observed in the renal cortex from these animals.


Renal development Angiotensin II Sodium intake Cell proliferation Extracellular matrix 



The authors thank Cleonice G.A. da Silva, Erika Delloiagono, and Rubens Fernando de Melo for expert technical assistance. Ana Paula C. Balbi was a recipient of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, DF, Brazil, fellowship, and Dr. Roberto Silva Costa and Dr. Terezila Machado Coimbra are recipients of Conselho Nacional de Desenvolvimento Científico e Tecnológico, DF, Brazil, fellowships. These results were presented in abstract form at the American Society of Nephrology Meeting, Philadelphia, Pa., November 2002.


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Copyright information

© IPNA 2004

Authors and Affiliations

  • Ana Paula C. Balbi
    • 1
  • Roberto S. Costa
    • 2
  • Terezila M. Coimbra
    • 1
  1. 1.Department of Physiology, Faculty of MedicineUniversity of Sao PauloSao PauloBrazil
  2. 2.Department of Pathology, Faculty of MedicineUniversity of Sao PauloSao PauloBrazil

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